Development of Microplate Concentration Measuring Method Aimed to Improve Sensitivity and Simplification

Manaka, Atsushi; Igarashi, Shukuro

doi:10.2116/bunsekikagaku.62.811

Cited by 1 publication

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“…The titration was carried out without reading the graduation of the burette. [9][10][11][12] The number of titrant droplets (each 0.05 mL) until the end point was counted for a semi-quantitative determination. 9 Dropping bottles are used for this purpose in a commercial titration set, e.g., 5 μg Ca mL -1 corresponding to one droplet in the titration of calcium with EDTA.…”

Section: Introductionmentioning

confidence: 99%

“…10 In a microplate concentration measuring method, the color change of a series of microwells with different amounts of titrant was used to obtain the end point of titration. 11,12 This method is simple and suitable for on-site analysis. However, the analytical precision is restricted by the number of wells, and thereby this method is semi-quantitative.…”

Section: Introductionmentioning

confidence: 99%

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Droplet-counting Microtitration System for Precise On-site Analysis

et al. 2018

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A new microtitration system based on the counting of titrant droplets has been developed for precise on-site analysis. The dropping rate was controlled by inserting a capillary tube as a flow resistance in a laboratory-made micropipette. The error of titration was 3% in a simulated titration with 20 droplets. The pre-addition of a titrant was proposed for precise titration within an error of 0.5%. The analytical performances were evaluated for chelate titration, redox titration and acid-base titration.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%