Development of micropatterned surfaces of poly(butylene succinate) by micromolding for guided tissue engineering

Abstract

Native tissues present complex architectures at the micro- and nanoscale that dictate their biological function. Several microfabrication techniques have been employed for engineering polymeric surfaces that could replicate in vitro these micro- and nanofeatures. In this study, biomimetic surfaces of poly(butylene succinate) (PBS) were engineered by a micromolding technique. After the optimization of the system parameters, 20 surfaces with different combinations of groove and ridge sizes were developed and characterized by scanning electron microscopy (SEM). The influence of the engineered microfeatures over the viability and attachment of human adipose derived adult stem cells (hASCs) was evaluated. hASCs cultured onto the engineered surfaces were demonstrated to remain viable for all tested patterns. SEM and immunostaining showed adequate attachment and spreading of the stem cells for all the patterned groove/ridge combinations. This study indicated that it is possible to engineer micropatterned surfaces of PBS and that the developed structures could have great potential for tissue engineering where cell alignment is an essential requisite.

Osteogenic differentiation of human mesenchymal stem cells in the absence of osteogenic supplements: A surface-roughness gradient study

“…Quite frequently, however, synthetic polymers, such as PCL, do not demonstrate particular bioactivity/affinity [11], which hinders the interaction of cells and tissues with the implant, ultimately limiting the effectiveness of the implantintegration in the body [12]. In order to obtain a specific cell response, tailoring of physical or chemical surface properties of the material is a valuable tool [12][13][14][15]. Among the different surface modifications that can be used to improve the materials' bioactivity, altering the surface roughness has been regarded as one of the most relevant approaches promoting osteointegration [16].…”

Review on PCL, PBS, and PCL/PBS blends containing carbon nanotubes

“…PCL was blended with various other biodegradable polymers in a number of studies [12][13][14][15][16][17][18][19][20][21][22]. Amongst various biodegradable polymers, PBS was the most interesting aliphatic polyester due to its relatively good melt processability, thermal and chemical resistance, biodegradability, and excellent mechanical properties, closely comparable to those of the widely-used polyethylene (PE) and polypropylene (PP) [4,5,[15][16][17][18][19][20][21][23][24][25][26][27][28][29][30][31][32][33][34][35][36]. Double crystalline PCL/PBS blends are particularly interesting because each component has an influence on the crystallization behaviour of the other component.…”

In vitro evaluation of PLLA/PBS sponges as a promisingbiodegradable scaffold for neural tissue engineering

“…PLLA is one of the most preferred polyesters in tissue engineering that has been used in many forms such as sponges, fibers, and films (Roether et al, 2002;Yang et al, 2004aYang et al, , 2004bWojasinski et al, 2013;Caldara et al, 2014). PBS has also been used in bone and cartilage tissue engineering with several applications including coating, micropatterning, and electrospinning (Ishioka et al, 2002;Coutinho et al, 2012;Wang et al, 2012;Wei et al, 2012;Arphavasin et al, 2013). Oudega et al (2001) investigated the effects of PLLA tubular scaffold implantation on spinal cord injury.…”