Development of Microfluidic Device for Electrical/Physical Characterization of Single Cell

Cho, Y.H.; Yamamoto, Takatoki; Sakai, Yasuyuki; Fujii, Teruo; Kim, Beomjoon

doi:10.1109/jmems.2005.863738

Cited by 48 publications

(41 citation statements)

References 14 publications

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“…Advances in this field have led to micro-devices that can measure the properties of single cells in a manner analogous to commercial flow cytometers. Different designs of micro-cytometers have been fabricated capable of measuring the optical and/or the electrical properties of single particles [68][69][70][74][75][76][77][78][79][80][81][82][83][84].…”

Section: Microfluidic Cytometrymentioning

confidence: 99%

Single cell dielectric spectroscopy

Morgan

Sun²,

Holmes³

et al. 2006

J. Phys. D: Appl. Phys.

378

313

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Over the last century a number of techniques have been developed which allow the measurement of the dielectric properties of biological particles in fluid suspension. The majority of these techniques are limited by the fact that they only provide an average value for the dielectric properties of a collection of particles. More recently, with the advent of microfabrication techniques and the Lab-on-a-chip, it has been possible to perform dielectric spectroscopic experiments on single biological particles suspended in physiological media. In this paper we review current methods for single cell dielectric spectroscopy. We also discuss alternative single cell dielectric measurement techniques, specifically the ac electrokinetic methods of dielectrophoresis and electrorotation. Single cell electrical impedance spectroscopy is also discussed with relevance to a microfabricated flow cytometer. We compare impedance spectroscopy data obtained from measurements made using a microfabricated flow cytometer with simulation data obtained using an equivalent circuit model for the device.

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Section: Microfluidic Cytometrymentioning

confidence: 99%

Single cell dielectric spectroscopy

Morgan

Sun²,

Holmes³

et al. 2006

J. Phys. D: Appl. Phys.

378

313

View full text Add to dashboard Cite

show abstract

“…The electrical and/or mechanical characterization of single cells is fundamental for understanding cell properties and has also been correlated with pathophysiological states in diseases, such as malaria [6][7][8][9][10][11][12] and cancer. [13][14][15][16][17][18][19][20] Advances in microfluidic technologies have led to the development of microdevices for single-cell electrical property characterization.…”

Section: Introductionmentioning

confidence: 99%

“…Hydrodynamic trapping methods have been employed in conjunction with -EIS for measuring cellular impedance properties over extended periods of time. 11,[48][49][50] Furthermore, two mechanisms utilizing vacuum aspiration 13,14 and electrode surface modification 51 were proposed to form tight cell adhesion on the measurement electrodes. In addition, microhole-based chips modified from patch-clamp devices have been proposed to address the leakage current issue by forming proper sealing between the aspirated cell and the aspiration channel.…”

Section: Introductionmentioning

confidence: 99%

“…For a more complete understanding of a cell's properties, it is desirable to perform both electrical and mechanical measurements on the same cell using the same device. The only device, which was reported to perform both impedance and mechanical characterization of single cells, 11 is complex in both design and microfabrication processes. In this device, cantilever deformation ratios were used to indicate cell mechanical properties, which are difficult to be translated into cell's Young's modulus.…”

Section: Introductionmentioning

confidence: 99%

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A microfluidic device for simultaneous electrical and mechanical measurements on single cells

et al. 2011

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This paper presents a microfluidic device for simultaneous mechanical and electrical characterization of single cells. The device performs two types of cellular characterization ͑impedance spectroscopy and micropipette aspiration͒ on a single chip to enable cell electrical and mechanical characterization. To investigate the performance of the device design, electrical and mechanical properties of MC-3T3 osteoblast cells were measured. Based on electrical models, membrane capacitance of MC-3T3 cells was determined to be 3.

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“…Single particle impedance measurement can be categorized as stationary or dynamic (flow-cytometry) measurements. Recently, Cho et al (2006) fabricated twin microcantilever arrays in microchannels for measuring the impedance of normal and abnormal red blood cells. Malleo et al (2007) developed a microfluidic chip to hydrodynamically capture single cells and analyse the kinetics of cell response to Streptolysin-O.…”

Section: Introductionmentioning

confidence: 99%

Digital signal processing methods for impedance microfluidic cytometry

Sun

Berkel

Green

et al. 2008

Microfluid Nanofluid

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Impedance microfluidic cytometry is a noninvasive, label-free technology that can characterize the dielectric properties of single particles (beads/cells) at high speed. In this paper we show how digital signal processing methods are applied to the impedance signals for noise removal and signal recovery in an impedance microfluidic cytometry. Two methods are used; correlation to identify typical signals from a particle and for a noisier environment, an adaptive filter is used to remove noise. The benefits of adaptive filtering are demonstrated quantitatively from the correlation coefficient and signal-to-noise ratio. Finally, the adaptive filtering method is compared to the Savitzky-Golay filtering method.

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Development of Microfluidic Device for Electrical/Physical Characterization of Single Cell

Cited by 48 publications

References 14 publications

Single cell dielectric spectroscopy

Single cell dielectric spectroscopy

A microfluidic device for simultaneous electrical and mechanical measurements on single cells

Digital signal processing methods for impedance microfluidic cytometry

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