Development of Methotrexate and Minocycline-Loaded Nanoparticles for the Effective Treatment of Rheumatoid Arthritis

Janakiraman, Kumar; Krishnaswami, Venkateshwaran; Sethuraman, Vaidevi; Natesan, Subramanian; Rajendran, Vijaya; Kandasamy, Ruckmani

doi:10.1208/s12249-019-1581-y

Cited by 16 publications

(9 citation statements)

References 42 publications

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“…Therefore, ESR, WBCs values were significantly decreased in arthritic rats after therapeutic use of nanoparticles. Our results were comparable to those of other previous studies as well (Janakiraman et al, 2020).…”

Section: Discussionsupporting

confidence: 93%

Untitled

2021

Pak. J. Pharm. Sci

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Rheumatoid arthritis is an inflammatory arthropathy, autoimmune in nature, leading to disability of joints involving structural destruction of articular bone and cartilage due to inflammation in synovium resulting in joint stiffness, swelling and pain. Nanomedicine has played a crucial role in improving the efficacy of treatment by controlling the release of pharmacologically active ingredients to increase bioavailability and achieve uniform and targeted delivery of drug. In this study, we prepared celecoxib, gingerol and oleanic acid loaded PLGA nanoparticles by solvent evaporation method and nanoparticles were characterized by particle size, zeta potential, polydispersity index, entrapement efficiency and FTIR. FCA is induced in right hand paw of rats for induction of arthritis. Celecoxib, gingerol and oleanic acid loaded PLGA nanoparticles coated with chitosan were given orally to rats for the evaluation of antiarthritic effect of this nanoformulation in rats. Animals were divided into six groups for 21 days trial. On 21 st day blood samples were collected for evaluation of hematological and lipid profile parameters. The data was subjected to statistical analysis by applying one way ANOVA and tukey test. At the end of study it was concluded that PLGA loaded celecoxib, gingerol and oleanic acid coated with chitosan have excellent effects in minimizing the side effects and increasing the therapeutic efficacy of drugs.

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Section: Discussionsupporting

confidence: 93%

Untitled

2021

Pak. J. Pharm. Sci

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“…The blank TDs did not exhibit significant cytotoxicity in the MDA-MB-231 cell culture at 1 mg/mL (Figure 5C). Consistent with the literature, 49 the dose-dependent cytotoxicity of MTX was observed in Raw 264.7 cell incubation with an IC 50 of 12 ng/mL. In contrast, the breast cancer cell line MDA-MB-231 is not sensitive to MTX due to the low expression level of reduced folate carrier, a transmembrane transporter for MTX cell uptake.…”

Section: Mtx Loading Efficiency and Stabilitysupporting

confidence: 89%

Rationally Designed Micellar Nanocarriers for the Delivery of Hydrophilic Methotrexate in Psoriasis Treatment

Guo

Shi

Wang

et al. 2020

ACS Appl. Bio Mater.

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Methotrexate (MTX) is broadly applied in the clinic for the treatment of cancers and autoimmune diseases. Targeted delivery of MTX is attractive to improve its efficacy and reduce offtarget toxicity. However, MTX encapsulation in the nanoparticle is challenging due to its high water solubility. We rationally designed a well-defined telodendrimer (TD) nanocarrier based on the MTX structure to sequester it in nanoparticles. Riboflavin (Rf) and positive charge groups were precisely conjugated on TD to form multivalent hydrogen bonds, π−π stacking, and electrostatic interactions with MTX. A reverse micelle approach was developed to preset MTX and TD interactions in the core of micelles, which ensures effective MTX loading upon dispersion into aqueous solution. As a result, the MTX loading capacity reaches over 20% (w/ w) in the optimized nanocarrier with a particle size of 20−30 nm. The nanoformulations sustain the release of MTX in a controlled manner and exhibit excellent hemocompatibility. The in vitro cellular uptake of MTX was significantly improved by the nanoformulations. The potency of MTX nanoformulations is comparable to that of the free MTX in cytotoxicity. A psoriasis-like skin inflammation model was induced in a mouse by imiquimod (IMQ) stimulation. MTX nanoformulations improved the psoriasis targeting and exhibited a superior long-lasting efficacy in reducing skin inflammation compared with the free MTX in psoriasis treatment.

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“…Methotrexate and minocycline-loaded nanoparticles were developed to relieve inflammation and disease progression/joints stiffness and control the bacterial infections associated with rheumatoid arthritis. 22 The pathogenesis of inflammatory skin diseases is complex and diverse. Whether the pathogenesis of GD may be related to immunity still needs a lot of data support and more in-depth research.…”

Section: Discussionmentioning

confidence: 99%

Bullous Grover’s Disease in a Chinese Tibetan Adolescent: A Case Report

Wang¹,

Luo²,

M³

et al. 2022

CCID

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Grover's disease (GD), also known as Transient acantholytic dermatosis, has no typical clinical rash features. It usually occurs in elderly white men but very rarely in China. This is a disease of acantholysis and dyskeratosis, which is usually considered to be spontaneous remission. The skin lesions of the disease are diverse, and the main symptom is severe itching. We have reported a case of GD in a 14-year-old Chinese Tibetan male whose clinical manifestations were pruritic red papule, generalized red papules, papulo vesicles and blisters ranging from millet rice to soybean size. Skin lesions change rapidly and variously. In order to confirm the diagnosis, we have done skin biopsies, immunofluorescence, dermoscopy, microscopy and other examinations. Pathological skin biopsy showed acantholysis. Intraepidermal blisters and the presence of blisters on the basal cells as well as under the stratum corneum can be observed on the same pathological section. Type IV collagen immunohistochemistry showed blisters in the epidermis. The diagnosis of GD depended on the exclusion of other diseases. After we performed whole exon sequencing (WES) on DNA from the patient's blood, pathogenic gene mutations were not found. Pustular psoriasis, Subcorneal pustular dermatosis, Herpesvirus infections, Dermatitis herpetiformis, Pemphigus vulgaris, Norwegian scabies, Darier's disease, and Hailey-Hailey disease were all excluded. We successfully treated adolescent GD with minocycline combined with methotrexate. The patient was followed up for 19 months without recurrence.

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Development of Methotrexate and Minocycline-Loaded Nanoparticles for the Effective Treatment of Rheumatoid Arthritis

Cited by 16 publications

References 42 publications

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Rationally Designed Micellar Nanocarriers for the Delivery of Hydrophilic Methotrexate in Psoriasis Treatment

Bullous Grover’s Disease in a Chinese Tibetan Adolescent: A Case Report

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