Development of Mast Cells and Importance of Their Tryptase and Chymase Serine Proteases in Inflammation and Wound Healing

Douaiher, Jeffrey; Succar, Julien; Lancerotto, Luca; Gurish, Michael F.; Orgill, Dennis P.; Hamilton, Matthew J.; Krilis, Steven A.; Stevens, Richard L

doi:10.1016/b978-0-12-800267-4.00006-7

Cited by 140 publications

(128 citation statements)

References 242 publications

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“…However, there is a claim that a third subset -only chymase-positive mast cells (MC C ) -exists [8] or even there is a wider spectrum of MCs subgroups, varying in the number and types of mediators they produce when activated [3]. In the skin, the MC TC population predominates [9].…”

Section: Introductionmentioning

confidence: 99%

“…in the intestinal mucosa and in the skin. In normal conditions they are found in subcutaneous fat and dermis, near blood vessels, hair follicles and nerves [2,3]. They contain granules with a vast array of mediators, i.e.…”

Section: Introductionmentioning

confidence: 99%

“…Tryptase, creating a complex with heparin, degrades fibrinogen in the wound healing process to prevent its further accumulation and thrombus formation. It regulates granulocyte and/or macrophage accumulation in tissues [3]. However, these two proteases have the ability to degrade many protein and peptides, which can lead to skin damage [10].…”

Section: Introductionmentioning

confidence: 99%

“…histamine, heparin, chymase, tryptase, etc. [1,3]. In the innate response, they can both detect the pathogens and release proper mediators to promote a pathogen-specific clearance mechanism.…”

Section: Introductionmentioning

confidence: 99%

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Mast cells in systemic and cutaneous lupus erythematosus

Kaczmarczyk-Sekuła¹,

Dyduch²,

Kostański³

et al. 2015

pjp

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Mast cells (MCs) are known to be regulators of inflammation and immunity, due to the released mediators and expressed cell surface molecules. Lupus erythematosus (LE) is a group of diseases which can be systemic or limited to the skin. Due to the fact that cytokines and chemokines produced by inflammatory cells contribute to the pathogenesis of LE, we quantified the number of mast cells present in the skin. The aim of the study was to compare the chymase-positive and tryptase-positive mast cell counts within skin biopsies from patients with systemic lupus erythematosus (SLE), discoid lupus erythematosus (DLE) and subacute cutaneous lupus erythematosus (SCLE). The material consisted of 45 skin biopsies: 6 with SLE, 34 with DLE and 5 with SCLE. Chymase-and tryptase-positive cells were stained by immunohistochemistry and counted. The mean count of chymase-positive mast cells was 85.14 hpf for the whole group, 35.83 for SLE, 88.48 for DLE and 121.6 for SCLE. The mean count of tryptase-positive cells was 120.05 hpf for the entire group, 59.17 for SLE, 126.42 for DLE and 149.8 for SCLE. The differences between groups were significant for chymase-and tryptase-positive cells.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mast cells in systemic and cutaneous lupus erythematosus

Kaczmarczyk-Sekuła¹,

Dyduch²,

Kostański³

et al. 2015

pjp

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“…Chymase secreted by mast cells is a type of serine protease and its release activates transforming growth factor (TGF)-β1 via paracellular secretion stimulation and fibrinolysin (3)(4)(5). A previous study demonstrated that chymase has a potential role in tissue fibrosis (6).…”

Section: Introductionmentioning

confidence: 99%

Mast cell chymase promotes hypertrophic scar fibroblast proliferation and collagen synthesis by activating TGF‑β1/Smads signaling pathway

Chen

Yang

et al. 2017

Exp Ther Med

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Abstract. The present study assessed the existence of mast cell chymase in hypertrophic scars and determined whether chymase promotes fibrosis via the transforming growth factor (TGF)-β1/Smads signaling pathway. Five patients with hypertrophic scars and another five patients subjected to repair and reconstruction of other tissue defects were included in the present study. To detect the existence of mast cells and mast cell chymase in hypertrophic scars, immunohistochemistry was employed. To test the effect of chymase on TGF-β1, angiotensin, and type I and III collagen mRNA expression in isolated hypertrophic scar fibroblasts in vitro, reverse-transcription quantitative PCR was performed. To investigate how chymase affects TGF-β1, phosphorylated (P)-Smad2/3 as well as Smad4 and Smad7 protein expression, western blot analysis was used. Mast cell chymase was identified to promote the mRNA expression of TGF-β1, angiotensin, and type I and III collagen in hypertrophic scar fibroblasts in a time-and dose-dependent manner. Furthermore, treatment with 60 ng/ml mast cell chymase for 12 h led to the upregulation of TGF-β1, P-Smad2/3, Smad4 and Smad7 in hypertrophic scar fibroblasts. The present study demonstrated that mast cells and chymase are present in hypertrophic scars, and chymase promotes hypertrophic scar fibroblast proliferation and collagen synthesis by activating the TGF-β1/Smads signaling pathway.

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Expression of the matrix metalloproteinases 2 and 9 in the rat small intestine during intrauterine and postnatal life

Reis

Soares

Gomes

2019

The Anatomical Record

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The expressions of matrix metalloproteinases 2 and 9 have been described during the development, as an example in heart and tooth but not in the small intestine yet. In this context, this study aimed to evaluate the expressions of MMP-2 and MMP-9 in the small intestine of Wistar rats during intrauterine (IU) and postnatal (PN) life. Expressions were determined on the 15th and 18th days of IU life and the 3rd, 10th, 17th, 25th, and 32nd days of PN life. Intestinal samples obtained from six animals were submitted to zymography, immunohistochemistry, and staining with Masson's trichrome. The results showed that MMP-2 and MMP-9 were not expressed during IU life; however, after birth, MMP-9 was immunolocalized in the goblet and mast cells. In conclusion, our results showed that MMP-2 and MMP-9 were not expressed in absorptive epithelial cells during the IU period of the small intestine but after birth, MMP-9 was expressed in the goblet cells, and mast cells present in the lamina propria.

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Development of Mast Cells and Importance of Their Tryptase and Chymase Serine Proteases in Inflammation and Wound Healing

Cited by 140 publications

References 242 publications

Mast cells in systemic and cutaneous lupus erythematosus

Mast cells in systemic and cutaneous lupus erythematosus

Mast cell chymase promotes hypertrophic scar fibroblast proliferation and collagen synthesis by activating TGF‑β1/Smads signaling pathway

Expression of the matrix metalloproteinases 2 and 9 in the rat small intestine during intrauterine and postnatal life

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