Development of magnetic anionic liposome/atelocollagen complexes for efficient magnetic drug targeting

Abstract

Magnetic nanoparticle-incorporated liposomes (magnetic liposomes) are considered a promising site-specific drug delivery carrier vehicle. With regard to their surface charge, magnetic anionic liposomes (Mag-AL) demonstrate little toxicity in comparison with magnetic cationic liposomes (Mag-CL), whereas their cellular association and uptake efficiency are low. In the current study, we constructed complexes of Mag-AL and atelocollagen (ATCOL), which is a biocompatible and minimally immunogenic biomaterial, to improve the cellular uptake properties of Mag-AL in vitro and in vivo. The cellular association and/or uptake of Mag-AL in RAW264 cells, a murine macrophage-like cell line, under a magnetic field was significantly increased when Mag-AL was complexed with ATCOL, and the highest cellular association was observed with complexes constructed using 5 µg/mL of ATCOL. The complexes showed liposome concentration-dependent and time-dependent cellular association under a magnetic field, and their cellular uptake efficiency was comparable with that of Mag-CL. In addition, Mag-CL showed significant cytotoxicity in a liposome concentration-dependent manner, whereas Mag-AL/ATCOL complexes produced no cytotoxic effect against RAW264 cells. Furthermore, the efficient cellular association of Mag-AL/ATCOL complexes in RAW264 cells was observed even in the presence of serum, and their liver accumulation was significantly increased at a magnetic field-exposed region after intravenous injection in rats. These results indicate that Mag-AL/ATCOL complexes could be a safe and efficient magnetic responsive drug carrier.

A Critical Scrutiny on Liposomal Nanoparticles Drug Carriers as Modelled by Topotecan Encapsulation and Release in Treating Cancer

“…Versions of the liposomal nanoparticles exist. These include magnetic nanoparticles [ 67 ]; thermosensitive liposome-in-gel [ 5 ]; transferrin receptor (TR)-targeted liposomal cisplatin [ 68 ]; magnetic thermosensitive cationic liposomes [ 69 ] and so on. Novel technologies have also been reported [ 70 , 71 ].…”

Liposome-polymer complex for drug delivery system and vaccine stabilization

“…Compared with magnetic cationic liposome (MCL), MAL showed both concentration-and time-dependent cellular uptake without any cytotoxic effects in vitro on RAW264 cells. However, the use of atelocollagen at concentrations (more than 20 μg/mL) will form aggregates at 37 C and inhibit cellular uptake due to large particle size [93].…”

Design and applications of liposome-in-gel as carriers for cancer therapy

“…coated MLs with atelocollagen to improve cellular uptake efficiency. After intravenous injection of atelocollagen-coated MLs, a disc-shaped magnet was placed close to the liver to accelerate ML enrichment inside the liver (Kono et al., 2017 ). Another study showed that magnetic anionic liposome (Mag-AL) binding was significantly increased under magnetic guidance in RAW264 cells (a murine macrophage-like cell line) compared to that of the control group.…”