Development of <i>Amsacta moorei </i>Entomopoxvirus in Ovarian and Hemocyte Cultures from <i>Estigmene acrea</i> Larvae

Abstract

Primary ovarian and hemocyte tissue cultures prepared from Estigmene acrea larvae were infected with Amsacta moorei entomopoxvirus. Infection of cultures was achieved with either purified virus or virus-containing culture medium from infected cultures. Virus replication and formation of virus-containing inclusions (VCI) were similar to that normally observed in vivo except that fewer virions appeared to be occluded in VCI.

Expression of polydnavirus genes under polydnavirus promoter regulation in insect larvae infected with baculovirus recombinants

“…The E-2 strain of Autographa ca]ifornica nucleopolyhedrovirus (AcMNPV) was used for infections requiring wild-type virus. A Trichoplusia ni cell line (Tn-5, BTI-Tn-SB1-4; Wickman & Nemerow, 1993) and an Estigmene acrea cell line (EaA, BTIEaA; Granados & Naughton, 1976) were maintained similarly.…”

Fitness-related traits of entomopoxviruses isolated from Adoxophyes honmai (Lepidoptera: Tortricidae) at three localities in Japan

“…Viral isolate pathogenicity can vary depending on the environment supporting its replication. It is known for Amsacta moorei EPV that the number of virions occluded per OB can vary among the tissues and between cultured cells and larvae where the virus replicated, which might cause a change in pathogenicity (Granados, 1973;Granados and Naughton, 1975). Therefore, we suspect that alternate host species for propagation may affect EPV pathogenicity through differences in the physiological environment of the host species.…”

In vitro host range of the Hz-1 nonoccluded virus in insect cell lines

“…Not enough insect cell lines from various tissues have been tested to distinguish between host range and tissue specificity of the virus but it was reported that an Estigmene acrea cell line (BTI-EAA) derived from hemocytes (Granados and Naughton 1975) also did not support Hz-1 replication (Ralston et al 1981). It is customary to state the tissue from which the cell line is derived but there are no reliable tests to verify that the cell line is truly of that particular origin.…”