Development of Low-Molecular Weight Collagen Peptide Complex with Glycosaminoglycan Components

Abstract

Enzymatic hydrolysis of biopolymers of the cartilage tissue was studied for obtaining a complex of type II collagen peptides and glycosaminoglycan oligosaccharides. Hydrothermal hydrolysis in a high pressure homogenizer followed by enzymatic hydrolysis of the cartilage tissue biopolymers with proteolytic enzyme preparation Karipazim yielded a complex of collagen peptides and glycosaminoglycan oligosaccharides with molecular weights of 240-720 Da. Low molecular weight of the components increases their bioavailability. Entering into the cells (chondrocytes), low-molecular-weight peptides, disaccharides, and oligosaccharides as structural elements of the matrix can participate in the formation of fibrils of collagen and proteoglycans. Exogenous substances replenish deficient components of the matrix and/or their concentrations, affect the formation and strengthen the cartilage tissue. Thus, using cattle and porcine hyaline cartilages, we prepared a complex of biopolymers with lower molecular weights in comparison with previously developed nutraceuticals.

Cytokine-Related Effect of Buccal-Delivered Collagen Peptide Incorporated in Mucoadhesive Films to Improve Female Skin Conditions

“…However, collagen is a high-molecular-weight protein and cannot be absorbed by the skin [10]. Thus, lower-molecular-weight collagen under 500 Da is composed of at most three amino acids, such as Gly-Pro-Hyp and Gly-Pro-Ala, as well as Hyp-Gly and Gly-Pro [12], called collagen hydrolysate, are frequently used to enhance the absorption of cosmetics [13] and effectiveness of collagen capsules as functional foods. On the other hand, The lower-molecular-weight collagen can be another protein due to it being just part of the peptide in whole collagen and when taken orally, as collagen passes through the gastrointestinal tract, it is mostly destroyed by digestive enzymes during first-pass metabolism [14].…”