“…These findings suggest that the novel recN PCR assay is capable of distinguishing authentic S. suis strains from those of other species including S. suis -like strains. In 2015, a loop-mediated isothermal amplification (LAMP) method targeting recN of S. suis was reported and revealed to be useful for detecting S. suis from raw pork meat [ 68 ]. As these novel PCR and LAMP assays become more popular, the diagnosis of S. suis infections will become more accurate, and our understanding of the epidemiology of this important zoonosis will improve.…”
Section: Methods For Identifying and Detecting
S Suis
mentioning
Streptococcus suis, a major porcine pathogen and an important zoonotic agent, is considered to be composed of phenotypically and genetically diverse strains. However, recent studies reported several “S. suis-like strains” that were identified as S. suis by commonly used methods for the identification of this bacterium, but were regarded as distinct species from S. suis according to the standards of several taxonomic analyses. Furthermore, it has been suggested that some S. suis-like strains can be assigned to several novel species. In this review, we discuss the current taxonomical situation of S. suis with a focus on (1) the classification history of the taxon of S. suis; (2) S. suis-like strains revealed by taxonomic analyses; (3) methods for detecting and identifying this species, including a novel method that can distinguish S. suis isolates from S. suis-like strains; and (4) current topics on the reclassification of S. suis-like strains.
“…These findings suggest that the novel recN PCR assay is capable of distinguishing authentic S. suis strains from those of other species including S. suis -like strains. In 2015, a loop-mediated isothermal amplification (LAMP) method targeting recN of S. suis was reported and revealed to be useful for detecting S. suis from raw pork meat [ 68 ]. As these novel PCR and LAMP assays become more popular, the diagnosis of S. suis infections will become more accurate, and our understanding of the epidemiology of this important zoonosis will improve.…”
Section: Methods For Identifying and Detecting
S Suis
mentioning
Streptococcus suis, a major porcine pathogen and an important zoonotic agent, is considered to be composed of phenotypically and genetically diverse strains. However, recent studies reported several “S. suis-like strains” that were identified as S. suis by commonly used methods for the identification of this bacterium, but were regarded as distinct species from S. suis according to the standards of several taxonomic analyses. Furthermore, it has been suggested that some S. suis-like strains can be assigned to several novel species. In this review, we discuss the current taxonomical situation of S. suis with a focus on (1) the classification history of the taxon of S. suis; (2) S. suis-like strains revealed by taxonomic analyses; (3) methods for detecting and identifying this species, including a novel method that can distinguish S. suis isolates from S. suis-like strains; and (4) current topics on the reclassification of S. suis-like strains.
“…Hence, it is a suitable target for the detection of relevant microorganisms by the molecular methods (Glazunova and others ; Tien and others ). A LAMP method was designed with primers targeting recN gene for the detection of Streptococcus suis in raw pork meat (Arai and others ). The primers were suitable for the amplification of all serotypes of S. suis under investigation, except S. suis serotypes 20, 22, 26, 32, 33, and 34 (taxonomically removed from the authentic S. suis ).…”
Section: Recent Applications Of Lamp Methods For the Quality Assessmementioning
Loop-mediated isothermal amplification (LAMP) is a novel method that amplifies target nucleic acids under isothermal conditions. It is a rapid, specific, and sensitive method, which does not require costly thermal cyclers for the detection of nucleic acids. Thus, it is suitable for on-site detection assays under low-resource settings. It can also be integrated on compact lab-on-a-chip devices for the development of micro-total analysis systems. This review discusses LAMP-based methods, as well as LAMP-based centrifugal, microfluidic, and other fluid-handling devices, which have been developed for the assessment of meat quality parameters that are related to the presence or absence of nucleic acids, for example, animal species identification and microbiological quality. Advances in improving the rapidity, specificity, and sensitivity of LAMP techniques for the assessment of these meat quality parameters are also discussed in this review.
“…Since S. suis serotype 2 and the other potential hazardous strains could be found even in the slaughtered pigs [13,14], contamination by S. suis in pork and pig organs should be taken into account as it could lead to a high risk of human infection. Loop-mediated isothermal amplification (LAMP) specific to S. suis , which was previously described elsewhere [15], was used to detect S. suis in this study. Since LAMP is simple, fast, and sensitive, this technique can be used as a surveillance tool for the detection of S. suis contamination.…”
Section: Introductionmentioning
confidence: 99%
“…Since LAMP is simple, fast, and sensitive, this technique can be used as a surveillance tool for the detection of S. suis contamination. Accordingly, LAMP techniques targeting the gene-encoding recombination/repair protein ( recN ) and the CPS synthesis gene specific for serotype 2 and 1/2 ( cps2J ) can be used for the detection of S. suis and S. suis serotype 2 or 1/2, respectively [15,16].…”
Background and Aim: Streptococcus suis is an important zoonotic pathogen that can cause serious diseases in both swine and humans worldwide, especially in Asian countries. Since the majority of human cases reported in Thailand were infected by the consumption of a raw pork dish, the microbial food safety hazard associated with raw meat has been a matter of concern. Therefore, this study aimed to investigate the contamination by S. suis in pork and edible pig organs sold in central Thailand.
Materials and Methods: In total, 88 raw pork and pig organ samples were purchased from markets, butcher shops, and supermarkets in central Thailand. The samples were examined using the loop-mediated isothermal amplification (LAMP) technique. LAMP reactions used for the detection of the DNA of S. suis (LAMPSS) and S. suis serotype 2 or 1/2 (LAMPSS2) were carried out according to previous studies.
Results: The percentage of LAMPSS-positive samples was as high as 85.23% (75/88) while the percentage of LAMPSS2- positive samples was 17.05% (15/88). The percentages of LAMPSS- and LAMPSS2-positive samples were relatively high in both pig organs (lung and heart) and meat (sliced pork and minced pork) compared with the previous report. Except one supermarket, LAMPSS-positive samples were found in all sources investigated in this study. The pork and pig organs obtained from the markets and the butcher shops additionally gave positive results for LAMPSS2.
Conclusion: Using LAMP techniques, high rate contamination of S. suis was found in raw pork and edible pig organs sold at different sources in central Thailand. The cross-contamination could have occurred through slaughtering, meat cutting, and meat handling processes. Therefore, consumers and people involved in the pig production industry should be aware of the potential hazards of S. suis infection; food safety education is crucial to prevent further infection.
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