Development of lateral flow assay based on anti-IBDV IgY for the rapid detection of Gumboro disease in poultry

Syahruni, Sari; Hartati, Yeni Wahyuni; Yusuf, Muhammad; Kusumawardani, Shinta; Wibawan, I Wayan Teguh; Arnafia, Wyanda; Sibit, Gowinda; Subroto, Toto

doi:10.1016/j.jviromet.2021.114065

Cited by 6 publications

(4 citation statements)

References 30 publications

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“…The IgY antibody was produced from the egg yolk of chickens immunized using the virus RBD as antigen in high purity (>90%) and yields (~20 mg per egg), with minimal environmental harm and infrastructure investment. Analysis of the SDS-PAGE showed two molecular patterns at 65 kDa and 27 kDa, corresponding to the IgY heavy and light chains, respectively [26]. We futher radiolabeled the IgY with iodine-131 to allow us to investigate several in vitro (e.g., immunoreactivity, cellular uptake) and in vivo (e.g., biodistribution, phar-macokinetic) parameters.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, IgY was found to be effective in blocking the internalization of Staphylococcus aureus into mammary epithelial cells, resulting in the deactivation of bacterial toxins [24]. In addition, chicken IgY possesses some advantages, such as the simple method for noninvasive local delivery by oral or intranasal administration, high specificity and avidity, limited adverse immune responses, capability to recognize more epitopes on a targeted antigen, the relatively quick production process, and high yield (40-80 mg antibody per egg) [25,26].…”

Section: Introductionmentioning

confidence: 99%

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Preclinical Evaluation of Chicken Egg Yolk Antibody (IgY) Anti-RBD Spike SARS-CoV-2—A Candidate for Passive Immunization against COVID-19

Wongso¹,

Mahendra²,

Arnafia³

et al. 2022

Vaccines

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The coronavirus disease 2019 (COVID-19) has become a substantial threat to the international health sector and the global economy. As of 26 December 2021, the number of mortalities resulting from COVID-19 exceeded 5.3 million worldwide. The absence of an effective non-vaccine treatment has prompted the quest for prophylactic agents that can be used to combat COVID-19. This study presents the feasibility of chicken egg yolk antibody (IgY) anti-receptor-binding domain (RBD) spike SARS-CoV-2 as a strong candidate to neutralize the virus for application in passive immunization. For the purpose of preclinical studies, we radiolabeled IgY anti-RBD spike SARS-CoV-2 with radionuclide iodine-131. This allowed us to evaluate several biological characteristics of IgY in vitro, in vivo, and ex vivo. The preclinical data suggest that IgY anti-RBD spike SARS-CoV-2 could specifically bind to the SARS-CoV-2 antigens; however, little uptake was observed in normal cells (MRC-5) (<2%). Furthermore, the ex vivo biodistribution study revealed that IgY predominantly accumulated in the trachea of normal mice compared to other organs. We also found that IgY possessed a good safety profile when used as an intranasal agent. Taken together, we propose that IgY anti-RBD spike SARS-CoV-2 has the potential for application in passive immunization against COVID-19.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preclinical Evaluation of Chicken Egg Yolk Antibody (IgY) Anti-RBD Spike SARS-CoV-2—A Candidate for Passive Immunization against COVID-19

Wongso¹,

Mahendra²,

Arnafia³

et al. 2022

Vaccines

Self Cite

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“…Also, handling IBDV post-outbreaks involves significant time and budgets. The programs of vaccination and early detection of virus is the important in control strategy of outbreak (Syahruni et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Detection and isolation of a recent infectious bursal disease virus from chicken farms in Egypt during 2021

Shahat

2023

Benha Veterinary Medical Journal

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Infectious bursal disease virus (IBDV) is considered as an economic challenge to the poultry industry. Monitoring of circulated recent IBDV is very significant in controlling the spreading of disease in Egypt. In this study, we are targeting VP2 gene of IBDV in bursal samples from 15 different infected chicken commercial farms in seven Egyptian governorates. Reverse transcriptase polymerase chain reaction (RT-PCR) was used for virus detection, 7 out of 15 examined farms from four governorates involving Qaluobia, Dakahlia, Sharkia, Gharbiya were IBDV positive. Isolation of IBDV was carried out by inoculation on chorioallantoic membrane of specific pathogen free embryonated chicken eggs (SPF-ECEs). The infectivity titration of the third passage of IBDV strains was 6.4, 5.6, 5.5 and 5.4 Log 10 EID50/0.1ml respectively for Dakahlia, Sharkia, Qaluobia and Gharbiya, then identified by RT-PCR and sequenced. The Neutralization index of Qaluobia isolate was 3. In conclusion, a recent Egyptian very virulent Infectious bursal disease virus (vvIBDV) strains antigenically different serotype 1 was circulated and further molecular characterization is required.

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“…Infectious bursal disease (IBD), originally identified as Gumboro disease, is an extremely contagious immunosuppressive disease in young chickens caused by infectious bursal disease virus (IBDV) ( 1 ). IBDV is the type species of the family Birnaviridae , which includes nonenveloped, icosahedral, double-stranded RNA (dsRNA) viruses that infect a variety of vertebrate and invertebrate hosts ( 2 ).…”

Section: Introductionmentioning

confidence: 99%

Phosphorylation of VP1 Mediated by CDK1-Cyclin B1 Facilitates Infectious Bursal Disease Virus Replication

Chen

et al. 2023

J Virol

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Development of lateral flow assay based on anti-IBDV IgY for the rapid detection of Gumboro disease in poultry

Cited by 6 publications

References 30 publications

Preclinical Evaluation of Chicken Egg Yolk Antibody (IgY) Anti-RBD Spike SARS-CoV-2—A Candidate for Passive Immunization against COVID-19

Preclinical Evaluation of Chicken Egg Yolk Antibody (IgY) Anti-RBD Spike SARS-CoV-2—A Candidate for Passive Immunization against COVID-19

Detection and isolation of a recent infectious bursal disease virus from chicken farms in Egypt during 2021

Phosphorylation of VP1 Mediated by CDK1-Cyclin B1 Facilitates Infectious Bursal Disease Virus Replication

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