Development of late blight resistant potatoes by cisgene stacking

Jo, Kwang‐Ryong; Chol-jun, Kim; Kim, Sungjin; Kim, Tok-Yong; Bergervoet, Marjan; Jongsma, Maarten A.; Visser, Richard G. F.; Jacobsen, E.; Vossen, Jack H.

doi:10.1186/1472-6750-14-50

Cited by 132 publications

(83 citation statements)

References 34 publications

(32 reference statements)

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“…Potato is advantaged with respect to other species because cisgenesis can be obtained without the use of any selectable marker gene, due the high transformation and regeneration rates that are necessary for this strategy. This is the case of the more recent potato genotypes carrying late blight resistance genes [32]. Also, the intragenic potatoes produced by Simplot are not indistinguishable from native ones, as they carry a combination of sequences not available in nature, but they are safe and not risky both for humans and for the environment.…”

Section: Cisgenic Potatoes In Organic Farming: Scientific Legislativmentioning

confidence: 99%

“…The sustainable control of this pathogen can be achieved only through genetic modification. Different late blight resistance genes were cloned from wild relatives (Solanum stoloniferum, S. bulbocastanum, S. demissum, and S. venturii) and introduced in several potato cisgenic lines [32,33,47,48] using a marker-free transformation approach [32,34]. Stacking of multiple R genes maximized durability of resistance and a correct spatial and temporal management where different improved lines may subsequently be introduced in a cultivation rotation scheme, supporting disease management and reducing fungicide use by over 80%.…”

Section: Not All Gm Potatoes Are Equalmentioning

confidence: 99%

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Effect of Organic Potato Farming on Human and Environmental Health and Benefits from New Plant Breeding Techniques. Is It Only a Matter of Public Acceptance?

Pacifico

Paris

2016

Sustainability

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Organic farming practices are commonly thought to reduce the environmental impact of agriculture and to preserve the naturalness of the products. Herein, we report the effect of crop management practices on nutritional and toxicological value of potato tubers. Comparative studies are often controversial and the results are dependent on genotype and methodological approach. Targeted analysis and "omics" strategies are discussed, pointing at the nutritional aspects and the corresponding biological and molecular processes involved. Organic farming supporters still do not accept the use of genetic modification to produce new varieties suited for organic agriculture and crop improvement by genetic engineering still sparks hot debate among various scientific and social factions whose major concern is the possible existence of unintended effects both on human and world health. In this context, the advent of "new plant breeding techniques" has reignited the discussion on genetic engineering and on the compatibility of the new technologies with an eco-friendly agriculture. Could cisgenic and genome-edited potatoes be new good options for organic agriculture? We discuss how these approaches can be used to address food security challenges and to overcome specific problems based on the biological characteristics of potato tubers, producing new varieties that can improve farmers' profit with a lower impact on public opinion. However, political, ethical, and social fears will probably persist much longer, mainly in Italy, historically a fiercely anti-GM country with a European leadership in organic food production and export.

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Section: Cisgenic Potatoes In Organic Farming: Scientific Legislativmentioning

confidence: 99%

Section: Not All Gm Potatoes Are Equalmentioning

confidence: 99%

Effect of Organic Potato Farming on Human and Environmental Health and Benefits from New Plant Breeding Techniques. Is It Only a Matter of Public Acceptance?

Pacifico

Paris

2016

Sustainability

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“…To date, over 20 NLR-type disease resistance genes have been identified from different solanaceous species, which confer resistance to infection by diverse and destructive pathogens and pests, including the oomycete Phytophthora infestans, tomato spotted wilt virus (TSWV), and potato cyst and root-knot nematodes (13,14). Several of these solanaceous NLR-type disease resistance genes have been deployed in agriculture through traditional breeding, cisgenesis, or transgenesis (14,15). For example, Rpi-blb2 has been introgressed into potato cultivars to confer broad-spectrum resistance to isolates of P. infestans (16).…”

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confidence: 99%

NLR network mediates immunity to diverse plant pathogens

Abd-El-Haliem

Bozkurt

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

356

548

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Both plants and animals rely on nucleotide-binding domain and leucine-rich repeat-containing (NLR) proteins to respond to invading pathogens and activate immune responses. An emerging concept of NLR function is that "sensor" NLR proteins are paired with "helper" NLRs to mediate immune signaling. However, our fundamental knowledge of sensor/helper NLRs in plants remains limited. In this study, we discovered a complex NLR immune network in which helper NLRs in the NRC (NLR required for cell death) family are functionally redundant but display distinct specificities toward different sensor NLRs that confer immunity to oomycetes, bacteria, viruses, nematodes, and insects. The helper NLR NRC4 is required for the function of several sensor NLRs, including Rpi-blb2, Mi-1.2, and R1, whereas NRC2 and NRC3 are required for the function of the sensor NLR Prf. Interestingly, NRC2, NRC3, and NRC4 redundantly contribute to the immunity mediated by other sensor NLRs, including Rx, Bs2, R8, and Sw5. NRC family and NRC-dependent NLRs are phylogenetically related and cluster into a well-supported superclade. Using extensive phylogenetic analysis, we discovered that the NRC superclade probably emerged over 100 Mya from an NLR pair that diversified to constitute up to one-half of the NLRs of asterids. These findings reveal a complex genetic network of NLRs and point to a link between evolutionary history and the mechanism of immune signaling. We propose that this NLR network increases the robustness of immune signaling to counteract rapidly evolving plant pathogens.immunity | host-microbe interactions | evolution

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“…This approach holds great appeal among potato breeding programs and companies worldwide since the genetic modification is achieved using genuine potato genes, and should consequently face less opposition during the deregulation and release process (Haverkort et al, 2008;Jo et al, 2014). As a matter of fact, one of such kind (cigenic/intragenic) potato is the innate potato which was deregulated recently in the USA.…”

Section: Introductionmentioning

confidence: 99%

Demystification of GM crop costs: releasing late blight resistant potato varieties as public goods in developing countries

Schiek

Hareau

Baguma

et al. 2016

IJBT

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A few studies have reported some of the costs associated with bringing to market genetically-modified (GM) crops but no comprehensive studies exist on the real cost of the entire process of developing and releasing one GM variety by a not-for-profit institution in a developing country for sustainable agriculture. Despite the lack of documented studies, it is commonly assumed that such an undertaking is cost prohibitive, based on mere hearsay, and on two private sector cost assessments. The present study assesses the costs and the time expenditures to two not-for-profit programs, one lead by CIP and the other by Cornell University, of developing a late blight resistant (LBr) potato variety for release in one developing country. CIP's costs run to $1.6 million over eight years, while Cornell's costs amount to $1.4 million over nine years. Exogenous disturbances might result in insignificant increases in cost, but can increase time expenditure significantly. A sensitivity analysis revealed that the total cost is markedly influenced by technical parameters determining the production and identification of the pre-commercial LBr transgenic event.

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Development of late blight resistant potatoes by cisgene stacking

Cited by 132 publications

References 34 publications

Effect of Organic Potato Farming on Human and Environmental Health and Benefits from New Plant Breeding Techniques. Is It Only a Matter of Public Acceptance?

Effect of Organic Potato Farming on Human and Environmental Health and Benefits from New Plant Breeding Techniques. Is It Only a Matter of Public Acceptance?

NLR network mediates immunity to diverse plant pathogens

Demystification of GM crop costs: releasing late blight resistant potato varieties as public goods in developing countries

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