Development of IVM‐IVF produced 8‐cell bovine embryos in simple, serum‐free media after conditioning or Co‐culture with buffalo rat liver cells

Rehman, Najeeb Ur; Collins, Andrew; Suh, T.K.; Wright, Raymond W.

doi:10.1002/mrd.1080380304

Cited by 13 publications

(4 citation statements)

References 30 publications

(27 reference statements)

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“…Menezo's B2 medium was used in this experiment because it is one of the most successful chemically defined media for culturing bovine embryos and contains no LIF or serum (Menezo et al, 1984). Although TCM-199 is an ideal medium for BRL cells (Rehman et al, 1994;Reed et al, 1996), it is not a good medium for early-stage bovine embryos in the absence of BRL cells (Hernandez-Ledezma et al, 1993). It was determined that LIF added to Menezo's B2 medium had no effect on percentage of embryos developing into blastocysts in B2 medium with or without serum.…”

Section: Discussionmentioning

confidence: 99%

“…However, primary cell cultures are often inconvenient and quite variable for use in embryo culture. Buffalo Rat Liver ( BRL) cells, a permanent cell line, have been used successfully for coculture of bovine embryos (Rehman et al, 1994;Van Inzen et al, 1995;Reed et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…An attractive alternative to coculture may be use of media conditioned by BRL cells (Hernandez-Ledezma et al, 1993). Although BRL conditioned media have been used for successful embryo development, rarely do results equal those of BRL cell coculture (Rehman et al, 1994;Trounson et al, 1994;Van Inzen et al, 1995). Van Inzen et al (1995) demonstrated that time of media conditioning affected embryonic development.…”

Section: Introductionmentioning

confidence: 99%

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Culture of bovine embryos in buffalo rat liver cell-conditioned media or with leukemia inhibitory factor.

Funston

Nauta

Seidel

1997

Journal of Animal Science

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Experiments were designed to study development of bovine embryos in TCM-199 medium conditioned by preculture with buffalo rat liver (BRL) cells. Conditioned media were harvested after BRL cells were cultured until confluency (CON), or for an additional 2 d with the same cells but new medium (CON-N) or the same medium (CON-S). Glucose in TCM-199 was depleted by BRL cells to different concentrations depending on coculture procedures: CON = 3.94 mM, CON-N = 1.67 mM, and CON-S = 1.11 mM glucose. In Exp. 1, development of bovine zygotes in CON-S resulted in fewer blastocysts than development in CON (10 vs 28%, P < .05); CON-N was not different from CON (26% blastocysts). Experiment 2 examined effects of moving embryos to a fresh drop of different or identical conditioned medium after culture for 3 d. Initial culture in CON-N and final culture in CON resulted in a greater (P < .01) number of blastocysts compared with the control of CON followed by CON (32 vs 19% blastocysts). This was not entirely due to changing from low to high glucose because adding glucose to CON-N after 3 d yielded only 18% blastocysts. To test the hypothesis that beneficial effects of BRL cell-conditioned media may be due to secretion of leukemia inhibiting factor (LIF), LIF was added to B2, a more appropriate medium than Medium-199 for culturing bovine embryos without conditioning or coculture with BRL cells. In the absence of serum, the percentage of blastocysts per cleaved embryo (17 to 28%) was not improved with LIF; however, the mean number of cells per blastocyst was higher (P < .05) in treatments with LIF (65 to 74 cells) than without LIF (47 cells). In B2 medium + 10% fetal calf serum, LIF was of no benefit; development to blastocysts was good with or without LIF (43% of cleaved).

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Culture of bovine embryos in buffalo rat liver cell-conditioned media or with leukemia inhibitory factor.

Funston

Nauta

Seidel

1997

Journal of Animal Science

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“…These may include conditioning of the medium by secretion of diffusible compounds such as differentiation inhibitory activity (DIA; Smith and Hooper, 1987;Gearing et al, 1987;Smith et al, 19881, removal, or modification of secreted factors or substances already present in the medium. Interaction of the embryonic cells with molecules expressed on the surface of the feeders may also play an important role, though there is no evidence to suggest a species specificity in this interaction, since both mouse ES cells and bovine embryo growth (Rehman et al, 1994) can be supported on BRL cells.…”

Section: Discussionmentioning

confidence: 99%

Initial culture behaviour of rat blastocysts on selected feeder cell lines

Ouhibi

Sullivan

English

et al. 1995

Molecular Reproduction Devel

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To increase our understanding of rat embryos in culture and to attempt the isolation of blastocyst-derived cell lines, we examined the initial growth behaviour of rat blastocysts from four strains of rat on four different feeder cell layers. The feeders used were a continuous cell line of murine embryonic fibroblasts (STO), primary mouse (MEF) or primary rat (REF) embryonic fibroblasts, and a continuous cell line of rat uterine epithelial cells (RUCs). A medium that gave optimum plating efficiencies for murine ES cells was used in the rat embryo culture. Each culture system allowed hatching and attachment of the blastocysts, that is, the behaviour was similar on each feeder and each strain for the first 2 days in culture. Subsequently, there was a rapid differentiation of the Inner Cell Mass (ICM) cells on fibroblastic feeder cell layers (STO > MEF > REF), and this was generally complete after 3-6 days in primary culture. On RUCs, the ICM was found to increase in size without differentiation up to and including day 4 and in some cases longer. Embryo-derived cells were obtained by disaggregating and passaging ICMs on REF and RUC feeders. Rounded, refractile, and epithelial-like cells were isolated on REF and colonies of ES-like cells on the RUCs. The ES-like cells were positive for expression of alkaline phosphatase and stage-specific embryonic-antigen 1. This is an important first step towards the derivation and culture of pluripotent ES cells from the rat.

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Development, chromosomal composition, and cell allocation of bovine cloned blastocyst derived from chemically assisted enucleation and cultured in conditioned media

Bunch

White

et al. 2004

Molecular Reproduction Devel

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Treatment of in vitro matured bovine oocytes with colcemid results in a membrane protrusion that contains maternal chromosomes, which can be easily removed by aspiration. Four experiments were designed to evaluate the overall and temporal effects of conditioned medium (CM) by bovine cumulus cells on development of nuclear transfer (NT) bovine embryos and to examine the chromosomal composition and allocation of inner cell mass (ICM) and trophectoderm (TE) of the subsequent blastocysts. The nuclear transfer embryos were cultured in various CR1aa media conditioned by preculture with bovine cumulus cells. Development to the blastocyst stage in BSA-containing CM (BCM) and serum-containing CM (SCM) were similar to co-culture group (24-30%). The 24 hr-conditioned BCM yielded higher blastocyst development than 48 and 72 hr-conditioned BCM. Temporary exposure of embryos to BCM and SCM followed by CR1aa was also studied. Morula and blastocyst development were not different among the groups cultured in BCM for 72, 96, and 168 hr, but were significantly higher (P < 0.01) than groups exposed to BCM for 24 and 48 hr, respectively. Blastocyst development in SCM for 24 hr (29%), 96 hr (25%), and 168 hr (27%) were much higher (P < 0.05) than those in SCM for 48 hr (12%) and 72 hr (10%). The analyses of chromosomal composition of the resulting blastocysts indicate approximately 80% of the blastocysts cultured in CR1aa with co-culture or groups initially exposed to BCM for 24 hr followed by culture in CR1aa were diploid. However, the incidence of diploidy were only 36-60% in SCM-cultured groups and groups cultured in BCM beyond 48 hr. Conditioned media did not affect the allocation of ICM and TE in the blastocyst. No difference was found in the ratio of inner cell mass to total cells in co-culture, BCM or SCM groups (0.424, 0.441, and 0.473, respectively). In conclusion, bovine cumulus cell-CM and CR1aa with co-culture supported comparable development and blastocyst ICM:total cell ratio of bovine NT embryos. However, CM affected the blastocyst chromosomal composition and induced higher mixploidy.

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Development of IVM‐IVF produced 8‐cell bovine embryos in simple, serum‐free media after conditioning or Co‐culture with buffalo rat liver cells

Cited by 13 publications

References 30 publications

Culture of bovine embryos in buffalo rat liver cell-conditioned media or with leukemia inhibitory factor.

Culture of bovine embryos in buffalo rat liver cell-conditioned media or with leukemia inhibitory factor.

Initial culture behaviour of rat blastocysts on selected feeder cell lines

Development, chromosomal composition, and cell allocation of bovine cloned blastocyst derived from chemically assisted enucleation and cultured in conditioned media

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