Development of insect resistant transgenic cotton lines expressing cry1EC gene from an insect bite and wound inducible promoter

Kumar, Manoj; Shukla, Anoop Kumar; Singh, Harpal; Tuli, Rakesh

doi:10.1016/j.jbiotec.2009.01.005

Cited by 31 publications

(18 citation statements)

References 23 publications

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“…6). Several reports describe the insertion of a high copy number of T-DNA in plant genome through Agrobacterium-mediated transformation (Maghuly et al 2008;Kumar et al 2009). Less variation in rolA and rolC plant morphology could be due to an almost equal copy number observed in these transformants, whereas wide variation in rolB transformants could be due to the higher variation in copy number observed in these plants.…”

Section: Resultsmentioning

confidence: 99%

Expression of rol genes in transgenic soybean (Glycine max L.) leads to changes in plant phenotype, leaf morphology, and flowering time

Zia

Mirza

Malik

et al. 2010

Plant Cell Tiss Organ Cult

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Soybean (Glycine max L.) cultivar NARC-4 was transformed with constructs carrying rolA, rolB, or rolC genes, each under the control of the Cauliflower Mosaic Virus 70S promoter. Cotyledonary nodes of soybean seeds were infected with Agrobacterium tumefaciens strain LBA4404 carrying one of the three rol genes, along with nptII in the plasmid pLBR. The efficiency of the transformation varied slightly among the three constructs, with frequencies of 6, 7, and 5% for the rolA, rolB, and rolC genes, respectively, being observed. Southern blot analysis confirmed the integration of rol genes in the soybean genome with varying numbers of copies of the transgene. All transformed plants showed enhanced rooting, but the number of adventitious roots was higher for transformants carrying the rolB transgene. rolA and rolC transformants showed dwarf phenotypes, clustered branching, and variations in leaf morphology. Furthermore, these plants flowered within a short period of time and produced lower numbers of flowers. rolB transformants showed variations in phenotype, including dwarf to semidwarf and shrubby growth, abnormal stem growth, short internodes, variations in leaf morphology, and greenish to yellowish-green colored leaves. These plants also flowered early, but dwarf plants produced low numbers of flowers, while shrubby plants produced high numbers of flowers, but these were mostly infertile.

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Section: Resultsmentioning

confidence: 99%

Expression of rol genes in transgenic soybean (Glycine max L.) leads to changes in plant phenotype, leaf morphology, and flowering time

Zia

Mirza

Malik

et al. 2010

Plant Cell Tiss Organ Cult

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“…Some insects such as silkworms and bees are of important economic value. However, a highly efficient inducible system that could be extensively used in insect genetic engineering research has not been established to date, and thus it is of utmost significance to construct a pathogenic inducible promoter in disease resistance breeding and gene therapy (7, 8).…”

Section: Introductionmentioning

confidence: 99%

Construction and Characterization of a Synthetic Baculovirus-inducible 39K Promoter

Dong

et al. 2018

Preprint

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The low expression activity and specificity of natural promoters limit the 22 applications of genetic engineering. To construct a highly efficient synthetic inducible 23 promoter in the Bombyx mori (Lepidoptera), we analyzed the regulatory elements and 24 functional regions of the B. mori nucleopolyhedrovirus (BmNPV) 39K promoter. The 25 results of truncated mutation analysis of the 39K promoter showed that the 26 transcriptional regulatory region spanning positions -573 to -274 and +1 to +62 is 27 essential for virus-inducible promoter activity. Further investigation using 28 electrophoretic mobility shift assay (EMSA) revealed that the baculovirus IE-1 29 protein binds to the 39K promoter at the -310 to -355 region, and transcription 30 activates the expression of 39K promoter assay. Finally, we successfully constructed a 31 synthetic inducible promoter that increase the virus-inducing activity of other 32 promoters using the baculovirus-inducible transcriptional activation region that binds 33 to specific core elements of 39K (i.e., spanning the region -310 to -355) . In summary, 34 we describes a novel, synthetic, and highly efficient biological tool, namely, a 35 virus-inducible 39K promoter, which provides endless possibilities for future gene 36 function research, gene therapy, and pest control in genetic engineering.37 38

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“…Transgenic cotton plants have been generated via Agrobacterium-mediated transformation of seedling-derived explants (Perlak et al 1990(Perlak et al , 1991Jenkins et al 1993;Wilson et al 1994;Balasubramani et al 2003;Leelavathi et al 2004;Guo et al 2007;Kumar et al 2009), shoot apices (Sanjaya et al 2005;Daud et al 2009), and embryogenic calli (Leelavathi et al 2004;Haq 2004;Wu et al 2005;Jin et al 2005;Jin et al 2006;Zhang et al 2006;Asad et al 2008;Khan et al 2010). Agrobacteriummediated transformation of cotton using embryogenic calli is preferred by many researchers (Leelavathi et al 2004;Wu et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Expression and inheritance of chimeric cry2AX1 gene in transgenic cotton plants generated through somatic embryogenesis

Sakthi

Naveenkumar

Deepikha

et al. 2015

In Vitro Cell.Dev.Biol.-Plant

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Transgenic cotton plants expressing a novel cry2AX1 gene consisting of sequences from the cry2Aa and cry2Ac genes, driven by the CaMV35S or the EnCaMV35S promoters, were generated through Agrobacterium-mediated transformation. Among 40 independent PCR-positive events, 14 produced Cry2AX1 protein at detectable levels. Cry2AX1 levels in T 0 plants ranged from 0.012 to 1.068 μg/g fresh leaf tissue. Insect bioassays on Cry2AX1-expressing T 0 transgenic lines showed 27.0 to 88.8% mortality of Helicoverpa armigera. Southern hybridization analysis revealed single locus integration of the cry2AX1 gene in two T 0 plants which caused 87.5 and 88.8% mortality in H. armigera. The presence and expression of the cry2AX1 gene were confirmed in T 1 progeny using PCR and quantitative ELISA. The highest expression level of Cry2AX1 protein recorded in the T 1 generation was 0.766 μg/g fresh leaf tissue, far less than the expression of Cry2Ab protein (23 μg/g leaf tissue) reported in the commercialized Bt cotton, Bollgard II. In spite of the low Cry2AX1 level, the mortality against H. armigera ranged between 33.3 and 85.7% in the T 1 generation. These results indicate that this novel cry2AX1 gene produced a potent insecticidal protein that was effective against H. armigera in transgenic cotton plants at very low concentrations.

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Development of insect resistant transgenic cotton lines expressing cry1EC gene from an insect bite and wound inducible promoter

Cited by 31 publications

References 23 publications

Expression of rol genes in transgenic soybean (Glycine max L.) leads to changes in plant phenotype, leaf morphology, and flowering time

Expression of rol genes in transgenic soybean (Glycine max L.) leads to changes in plant phenotype, leaf morphology, and flowering time

Construction and Characterization of a Synthetic Baculovirus-inducible 39K Promoter

Expression and inheritance of chimeric cry2AX1 gene in transgenic cotton plants generated through somatic embryogenesis

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