Development of insect-resistant transgenic indica rice with a synthetic cry1C* gene

Tang, Wei; Chen, Hao; Xu, Caiguo; Li, Xianghua; Lin, Yongjun; Zhang, Qifa

doi:10.1007/s11032-006-9002-9

Cited by 192 publications

(154 citation statements)

References 44 publications

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“…S. biformis feeds on young plants by lacerating the green tissue of leaves, and outbreaks have often been reported in China, India, and Bangladesh (Nugaliyadde and Heinrichs 1984, Dale 1994, Pathak and Khan 1994. Since the 1990s, numerous rice genotypes expressing various insect-active crystal (Cry) proteins derived from the bacterium Bacillus thuringiensis Berliner (Bt) (referred as Bt rice hereafter) have been developed with resistance to one or more rice lepidopterous pests, the most important of which are the yellow stem borer, Scirpophaga incertula (Walker) (Lepidoptera: Pyralidae), the striped stem borer, Chilo suppressalis (Walker) (Lepidoptera: Crambidae), and the leaffolder, Cnaphalocrocis medinalis (Guené e) (Lepidoptera: Pyralidae) (reviewed by Breitler et al 2004, High et al 2004, Ramesh et al 2004, Riaz et al 2006, Tang et al 2006, Rahman et al 2007. Since the late 1990s, Þeld trials of various Bt rice genotypes expressing Cry1Ab protein (Shu et al 2000;Ye et al 2001aYe et al , 2003, a fusion protein of Cry1Ab/Cry1Ac (Ye et al 2001b), Cry2A, Cry1Ac, and Cry9C , Cry1C (Tang et al 2006), and a fusion protein of Cry1Ac/CpTI (Han et al 2007) have been conducted in China.…”

Section: Abstract Bt Rice Nontarget Insect Rice Thrip Stenchaetotmentioning

confidence: 99%

Impacts of SixBtRice Lines on Nontarget Rice Feeding Thrips Under Laboratory and Field Conditions

Akhtar¹,

Tian²,

Chen³

et al. 2010

Environ Entomol

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Nontarget impacts of six transgenic Bt rice lines (expressing the Cry1Ab or Cry1Ab/ Cry1Ac protein) on the thrips, Stenchaetothrips biformis (Bagnall), attacking the rice seedling and tillering stages, were evaluated under laboratory and Þeld conditions. Laboratory results showed relatively longer larval, pupal development and preoviposition durations of S. biformis. Although it had a shorter oviposition period, female adult longevity and less total laid eggs were found when fed on some tested Bt rice in comparison to non-Bt controls. S. biformis population dynamics in Bt and non-Bt plots were monitored using the plastic bag and beat plate methods. In the Þeld, the temporal patterns of S. biformis population changes were similar between tested Bt rice lines and their respective control; however, the total number of S. biformis individuals collected from the Bt plots were signiÞcantly less or the same, varying from variety to variety, compared with those from the non-Bt plots. ELISA results showed that the Bt insecticidal protein could be transferred from Bt rice to the thrips, and the concentrations of the protein in rice leaves and thrips were not signiÞcantly correlated with some important biological parameters of the thrip. In addition, the potential effects of Bt rice on the abundance of S. biformis candidate predators are also discussed. In conclusion, our results show that the six Bt rice lines assessed may be less preferable host plants to S. biformis at the individual and population levels in comparison to the non-Bt rice plants.

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Section: Abstract Bt Rice Nontarget Insect Rice Thrip Stenchaetotmentioning

confidence: 99%

Impacts of SixBtRice Lines on Nontarget Rice Feeding Thrips Under Laboratory and Field Conditions

Akhtar¹,

Tian²,

Chen³

et al. 2010

Environ Entomol

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“…TT51 is a transgenic rice line expressing a Bt fusion gene derived from cry1Ab and cry1Ac under the control of the rice actinI promoter [8]. T2A-1 expresses a synthesized cry2A gene, whereas T1C-19 expresses a synthesized cry1C gene, both driven by the maize ubiquitin promoter [12,20]. All the rice lines were gifted by Lin YongJun, National Key Laboratory of Crop Genetic Improvement, Wuhan, China.…”

Section: Plant Materialsmentioning

confidence: 99%

Field evaluation of effects of transgenic cry1Ab/cry1Ac, cry1C and cry2A rice on Cnaphalocrocis medinalis and its arthropod predators

Han

et al. 2011

Sci. China Life Sci.

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“…However, documents on the relationship between transgene expression and crossing transmission are very limited up to date. Several reports described the expression behaviour of transgenes in rice crossbreeding in recent years, which giving the opinion that at least the same transgene expression level in hybrids, if no more than that of the transgenic parents, could be expected (Chen et al,2005;Tang et al, 2006;Wang and Lin, 2007). But the above results were established on the base of transgenic donors containing single-copy exotic gene and transformed by Agrobacterium tumefaciens method.…”

Section: Stability Of Transgene Expression In Crossing Transmissionmentioning

confidence: 99%

Integration and Expression Stability of Transgenes in Hybriding Transmission of Transgenic Rice Plants Produced by Particle Bombardment

Zhao¹,

Guo²,

Wang³

et al. 2011

mpb

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Four transgenic rice lines TR 5, TR 6, Ming B and Jingyin 119 obtained via particle bombardment were used as transgene donors to create hybrids. The integration and expression stability of exotic bar and cecropin B gene in conventional hybriding transmission were investigated by Southern and Northern blotting analyses. The selection marker bar gene was transferred to all hybrids under selection of Basta herbicide. Loss or gain of small hybridization bands (no more than 2.0 kb) of bar gene occurred in some hybrids, but the difference in integration sites of bar gene copies did not influence their stable expression. The non-selection gene cecropin B was stably transferred from the four transgene donors to their resulting hybrids, but expression level was very different. Silencing of cecropin B gene occurred in some hybrids from TR 5, TR 6 and Ming B. In the transgene donor Jingyin 119 and all its resulting hybrids, cecropin B and bar gene were stably expressed. We concluded that the stability of transgene during crossbreeding transmission is mainly determined by the primary transgenic donors and may be affected by recombination.

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Development of insect-resistant transgenic indica rice with a synthetic cry1C* gene

Cited by 192 publications

References 44 publications

Impacts of SixBtRice Lines on Nontarget Rice Feeding Thrips Under Laboratory and Field Conditions

Impacts of SixBtRice Lines on Nontarget Rice Feeding Thrips Under Laboratory and Field Conditions

Field evaluation of effects of transgenic cry1Ab/cry1Ac, cry1C and cry2A rice on Cnaphalocrocis medinalis and its arthropod predators

Integration and Expression Stability of Transgenes in Hybriding Transmission of Transgenic Rice Plants Produced by Particle Bombardment

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