Development of injectable hydrogels based on human amniotic membrane and polyethyleneglycol-modified nanosilicates for tissue engineering applications

Amnion-derived hydrogels as a versatile platform for regenerative therapy: from lab to market

“…The processing steps of AM tissue into a temperature-responsive injectable hydrogel include: 1) dissection of AM from underlying chorion tissue, 2) washing blood clots, 3) decellularization of AM tissue, 4) lyophilization and pulverization, 5) enzymatic digestion with pepsin, 6) incubation of pH-neutralized dAM solution at physiologic temperature ( Kafili et al, 2022 ). Figure 3 shows these steps and the potential applications of the derived hydrogels.…”

“… (A) Processing of dAM tissue into temperature-responsive hydrogels through dissection from placenta tissue, decellularization, lyophilization, pulverization, enzymatic digestion by pepsin in an acidic solution, and gel formation after incubation of pH-neutralized dAM solution at the physiologic temperature [Reprinted from Kafili et al (2022) , Copyright (2023), with permission from Elsevier]. (B) Tissue engineering applications of AM/dAM hydrogels.…”

“…On the other hand, the addition of nanosilicates cooperates in the acceleration of cell proliferation and migration as a result of the bioactivity of ions degraded from the nanosilicates ( Kafili et al, 2023b ). In an attempt to improve the dispersibility of Laponite in dAM hydrogels, we have examined the effect of stabilization of nanosilicates by amine-terminated polyethylene glycol (AT-PEG) ( Kafili et al, 2022 ). We have demonstrated that layer-by-layer self-assembly of collagen fibers and Laponite clusters occurs in Laponite-containing dAM hydrogels due to the disparate hydrophilic nature of nanosilicates and the dAM matrix.…”

“…We have demonstrated that layer-by-layer self-assembly of collagen fibers and Laponite clusters occurs in Laponite-containing dAM hydrogels due to the disparate hydrophilic nature of nanosilicates and the dAM matrix. The coating of Laponite nanosilicates with the hydrophilic AT-PEG agent improves their distribution in the hydrophilic dAM matrix ( Kafili et al, 2022 ). Therefore, dAM hydrogels containing PEG-modified nanosilicates are a potential candidate for TE applications, particularly for skin regeneration.…”

Injectable Near-Infrared Photothermal Responsive Drug-Loaded Multiwalled Carbon Nanotube Hydrogels for Spinal Cord Injury Repair

“…In addition, cations such as Ca 2+ can regulate the mechanical properties of the GG hydrogel. , Injectable hydrogels are a new type of hydrogel system developed in recent years that can be implanted into the body through injection. It is easily filled into irregularly shaped defect sites with minimal surgical trauma, effectively avoiding the risks of infection and possible secondary surgeries. , The mechanical properties of injectable hydrogels are also closer to the mechanical properties of normal spinal cord tissue, − but some of its shortcomings also limit its application. On the one hand, cell infiltration is blocked due to the lack of axon channels for crossing the hydrogel, resulting in a limited penetration of axon growth into the hydrogel.…”