Development of injectable graphene oxide/laponite/gelatin hydrogel containing Wharton's jelly mesenchymal stem cells for treatment of oxidative stress-damaged cardiomyocytes

Cheng, Yi-Hsien; Cheng, Sui Sun; Chen, Hsin-Ho; Hsu, Wei-Chia

doi:10.1016/j.colsurfb.2021.112150

Cited by 15 publications

(12 citation statements)

References 46 publications

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“…Unlike other existing MSCs, Wharton’s jelly mesenchymal stem cells (WJ-MSCs) show outstanding advantages involving immune-regulation and immune-evasion capacities, differentiation abilities, superior cell proliferation, relatively young cell type and ease of sourcing. The injectable GO/laponite/gelatin (GO-LG) hydrogel containing WJ-MSCs could induce cardiomyocyte proliferation under oxidative stress and decrease inflammation levels …”

Section: Hydrogels For Restoring Cardiac Functionmentioning

confidence: 99%

“…Involving graphene oxide (GO), reductive GO (rGO), carbon nanofibers (CNF), single-wall carbon nanotubes (SWCNTs) and graphene quantum dots, carbon nanomaterials emerge as promising conductive elements to be added into injectable hydrogels to endow them with electrical conductivity. Except with the tunable mechanical property for cell attachment, GO could reduce levels of intracellular ROS and shift macrophages from M1 (produce pro-inflammatory cytokines) to M2 (secrete anti-inflammatory cytokines) for restoring cardiac function . Adding GO nanomaterials endows oligo(poly(ethylene glycol) fumarate) (OPF) hydrogels with mechanical support and electric connection via upregulating gap junction associated proteins and Cx43 generation .…”

Section: Hydrogels With Heart-specific Mimicry and Functionalitymentioning

confidence: 99%

“…The injectable GO/laponite/ gelatin (GO-LG) hydrogel containing WJ-MSCs could induce cardiomyocyte proliferation under oxidative stress and decrease inflammation levels. 49 4.2.2. Induced Pluripotent Stem Cells.…”

Section: Mesenchymal Stromal Cellsmentioning

confidence: 99%

“…Except with the tunable mechanical property for cell attachment, GO could reduce levels of intracellular ROS and shift macrophages from M1 (produce pro-inflammatory cytokines) to M2 (secrete anti-inflammatory cytokines) for restoring cardiac function. 49 Adding GO nanomaterials endows oligo(poly(ethylene glycol) fumarate) (OPF) hydrogels with mechanical support and electric connection via upregulating gap junction associated proteins and Cx43 generation. 50 Compared with GO, rGO has increased electrical conductivity and good dispersion.…”

Section: Conductivitymentioning

confidence: 99%

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Insight into Heart-Tailored Architectures of Hydrogel to Restore Cardiac Functions after Myocardial Infarction

et al. 2022

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With permanent heart muscle injury or death, myocardial infarction (MI) is complicated by inflammatory, proliferation and remodeling phases from both the early ischemic period and subsequent infarct expansion. Though in situ re-establishment of blood flow to the infarct zone and delays of the ventricular remodeling process are current treatment options of MI, they fail to address massive loss of viable cardiomyocytes while transplanting stem cells to regenerate heart is hindered by their poor retention in the infarct bed. Equipped with heart-specific mimicry and extracellular matrix (ECM)-like functionality on the network structure, hydrogels leveraging tissue-matching biomechanics and biocompatibility can mechanically constrain the infarct and act as localized transport of bioactive ingredients to refresh the dysfunctional heart under the constant cyclic stress. Given diverse characteristics of hydrogel including conductivity, anisotropy, adhesiveness, biodegradability, self-healing and mechanical properties driving local cardiac repair, we aim to investigate and conclude the dynamic balance between ordered architectures of hydrogels and the post-MI pathological milieu. Additionally, our review summarizes advantages of heart-tailored architectures of hydrogels in cardiac repair following MI. Finally, we propose challenges and prospects in clinical translation of hydrogels to draw theoretical guidance on cardiac repair and regeneration after MI.

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Section: Hydrogels For Restoring Cardiac Functionmentioning

confidence: 99%

Section: Hydrogels With Heart-specific Mimicry and Functionalitymentioning

confidence: 99%

Section: Mesenchymal Stromal Cellsmentioning

confidence: 99%

Section: Conductivitymentioning

confidence: 99%

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Insight into Heart-Tailored Architectures of Hydrogel to Restore Cardiac Functions after Myocardial Infarction

et al. 2022

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“…GO has been reported to inhibit the apoptosis of mouse stem cells and enhance their functional diversity 17 . However, small GO flakes and highly reduced rGO decreased MSCs proliferation and induced their apoptosis 18 . Apoptosis impairs the efficacy of stem cells in the treatment of diabetic wounds 19,20 .…”

Section: Introductionmentioning

confidence: 99%

Graphene oxide accelerates diabetic wound repair by inhibiting apoptosis of Ad‐MSCs via Linc00324/miR‐7977/STK4 pathway

Wang

Yang

et al. 2022

The FASEB Journal

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Many studies have shown that graphene oxide (GO) promotes proliferation and differentiation of a variety of stem cells. However, its effect on adipose‐derived mesenchymal stem cell (Ad‐MSCs) apoptosis is still unclear. Apoptosis is a significant factor affecting stem cell‐based treatment of diabetic wounds. Therefore, we explored the effect of GO on Ad‐MSC apoptosis and diabetic wound healing. In this study, qRT‐PCR was used to detect Ad‐MSC expression of LncRNAs, miRNAs, and mRNAs under high‐glucose environment. RNA immunoprecipitation (RIP), RNA pull‐down, and luciferase assays were used to detect interactions of specific lncRNAs, miRNAs, and mRNAs. The effects of GO on Ad‐MSC apoptosis were explored by flow cytometry, TUNEL assay, and Western blot. A diabetic wound model was used to explore the function of Linc00324 on Ad‐MSC reparative properties in vivo. As a result, GO inhibited high glucose‐induced apoptosis in Ad‐MSCs, and Linc00324 contributed to the anti‐apoptotic effect of GO. RIP and RNA pull‐down confirmed that Linc00324 directly interacted with miR‐7977, functioning as a miRNA sponge to regulate expression of the miR‐7977 target gene STK4 (MST1) and downstream signaling pathways. In addition, GO reduced the apoptosis of Ad‐MSCs in wounds and promoted wound healing. Taken together, these findings suggest GO may be a superior auxiliary material for Ad‐MSCs to facilitate diabetic wound healing via the Linc00324/miR‐7977/STK4 pathway.

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Reactive Oxygen Species Scavenging Nanomedicine for the Treatment of Ischemic Heart Disease

Zhang

Dalan

et al. 2022

Advanced Materials

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Ischemic heart disease (IHD) is the leading cause of disability and mortality worldwide. Reactive oxygen species (ROS) have been shown to play key roles in the progression of diabetes, hypertension, and hypercholesterolemia, which are independent risk factors that lead to atherosclerosis and the development of IHD. Engineered biomaterial‐based nanomedicines are under extensive investigation and exploration, serving as smart and multifunctional nanocarriers for synergistic therapeutic effect. Capitalizing on cell/molecule‐targeting drug delivery, nanomedicines present enhanced specificity and safety with favorable pharmacokinetics and pharmacodynamics. Herein, the roles of ROS in both IHD and its risk factors are discussed, highlighting cardiovascular medications that have antioxidant properties, and summarizing the advantages, properties, and recent achievements of nanomedicines that have ROS scavenging capacity for the treatment of diabetes, hypertension, hypercholesterolemia, atherosclerosis, ischemia/reperfusion, and myocardial infarction. Finally, the current challenges of nanomedicines for ROS‐scavenging treatment of IHD and possible future directions are discussed from a clinical perspective.

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Development of injectable graphene oxide/laponite/gelatin hydrogel containing Wharton's jelly mesenchymal stem cells for treatment of oxidative stress-damaged cardiomyocytes

Cited by 15 publications

References 46 publications

Insight into Heart-Tailored Architectures of Hydrogel to Restore Cardiac Functions after Myocardial Infarction

Insight into Heart-Tailored Architectures of Hydrogel to Restore Cardiac Functions after Myocardial Infarction

Graphene oxide accelerates diabetic wound repair by inhibiting apoptosis of Ad‐MSCs via Linc00324/miR‐7977/STK4 pathway

Reactive Oxygen Species Scavenging Nanomedicine for the Treatment of Ischemic Heart Disease

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