DEVELOPMENT OF in vitro REGENERATION AND Agrobacterium MEDIATED TRANSFORMATION SYSTEMS FOR Moringa oleifera PLANT

Abbas, Dina E.

doi:10.21608/ejgc.2014.9935

Cited by 4 publications

(4 citation statements)

References 16 publications

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“…No GUS expression was detected in the other 19 plants or non-transgenic controls. The efficiency was higher than that reported by Abbas [19]. The plants negative for GUS expression may have been derived from untransformed cells that survived within the Hyg-resistant calli.…”

Section: Gus Staining Analysiscontrasting

confidence: 53%

“…The expression of gus in drumstick was comparable to that of the reference genes ( Figure 5), indicating that the plasmid pCAMBIA 1301 had been introduced successfully into drumstick cells and that gus had been integrated into the genome. In the previous report, PCR and GUS histochemical assay were used to determine drumstick transformation efficiency [19]. However, besides these two techniques, Southern blot and RT-qPCR were used in this study as well.…”

Section: Molecular Analysismentioning

confidence: 99%

“…However, limited reports are available in genetic transformation of drumstick. To our knowledge, only one genetic transformation protocol for drumstick has been reported so far [19].…”

Section: Introductionmentioning

confidence: 99%

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Agrobacterium tumefaciens-mediated transformation of drumstick (Moringa oleifera Lam.)

Zhang

Lin

Chen

et al. 2017

Biotechnology & Biotechnological Equipment

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Drumstick (Moringa oleifera Lam.) is an industrially important tree species. Due to the availability of an efficient regeneration system, the present study was undertaken to develop a reliable and stable Agrobacterium tumefaciens-mediated transformation system for the genetic improvement of drumstick. A. tumefaciens strain EHA105, harbouring the plasmid pCAMBIA1301 containing the hygromycin phosphotransferase II and ß-glucuronidase genes driven by the CaMV 35S promoter, was used to establish a transformation system in drumstick. The use of vacuum infiltration-assisted Agrobacterium infection (excluding a pre-culture step) and co-cultivation for two days significantly increased the transformation frequency. Genomic integration and transgene expression were confirmed by b-glucuronidase (GUS) assays, polymerase chain reaction (PCR), Southern blot analysis and real-time quantitative PCR (RT-qPCR). This transformation protocol provides a basis for the future development of genetic engineering techniques to improve the performance of drumstick.

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Section: Gus Staining Analysiscontrasting

confidence: 53%

Section: Molecular Analysismentioning

confidence: 99%

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Agrobacterium tumefaciens-mediated transformation of drumstick (Moringa oleifera Lam.)

Zhang

Lin

Chen

et al. 2017

Biotechnology & Biotechnological Equipment

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“…The highest shoot multiplication by nodal segments obtained from upper parts of ex vitro grown plants was reported by Salem (2015). Abbas (2014) studied the development of multiple shoots through the nodal segment obtained from seedlings grown in vitro. To this end, employing plant tissue culture for the supply of large numbers of high-quality planting materials in a medium containing different combinations of growth regulators is proposed in this study.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation in Moringa oleifera Lam for high-throughput Multiplication

2020

EJPB

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Moringa oleifera is the most widely cultivated species of the Moringaceae family Attributed to several medicinal uses and a high nutritional value. An efficient in vitro clonal propagation methodology for Moringa oleifera (PKM1 Variety) was developed using nodal explants of young seedlings grown ex vitro. Nodes were cultured in Murashige and Skoog (MS) medium with different combinations of plant growth regulators. Multiple shoots were successfully achieved by culturing nodal explants in a medium containing different concentrations of 6-Benzylaminopurine (BAP) and Kinetin (Kin) in combination with naphthalene acetic acid (NAA). BAP at 2mgL -1 was considered optimal for generating a maximum an average of 6.03±0.21 auxillary shoots per explants after 60 days of culture inoculation. A high rate of multiplication has been established by routine subculture on a similar shoot induction medium. Maximum numbers of individual roots were established on a medium containing indole-3-butyric acid (IBA) at 1.5mgL -1 . Primary hardening was done in pots containing the potting mixture and transferred plantlets were covered with clear polythene bags. Seventy per-cent of the rooted plants survived and secondary hardening was carried out after 15 days in a shaded greenhouse.

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Moringa oleifera: Biosynthesis strategies for enhanced metabolites and role in green nanoparticle synthesis

Choudhary,

Kumari,

Kachhwaha

et al. 2024

South African Journal of Botany

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DEVELOPMENT OF in vitro REGENERATION AND Agrobacterium MEDIATED TRANSFORMATION SYSTEMS FOR Moringa oleifera PLANT

Cited by 4 publications

References 16 publications

Agrobacterium tumefaciens-mediated transformation of drumstick (Moringa oleifera Lam.)

Agrobacterium tumefaciens-mediated transformation of drumstick (Moringa oleifera Lam.)

Micropropagation in Moringa oleifera Lam for high-throughput Multiplication

Moringa oleifera: Biosynthesis strategies for enhanced metabolites and role in green nanoparticle synthesis

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