Development of in vitro derived bovine embryos following pronuclear transplantation and in vitro culture

Westhusin, Mark; Azambuja, Ricardo

doi:10.1016/s0378-4320(96)01572-2

Cited by 2 publications

(2 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PT had no detrimental effect on cleavage and blastocyst development rates. This is in agreement with results obtained after pronuclear transfer in embryos of cattle produced by in vitro fertilization (Westhusin and De Azambuja, 1996).…”

Section: Discussionsupporting

confidence: 93%

“…Pronuclear transfer (McGrath and Solter, 1984;Westhusin and De Azambuja, 1996) was performed in HSOF with 7.5 g/mL cytochalasin B. Four experimental groups were produced: two in the PT control group, where removed karyoplast (pronucleus and adjacent cytoplasm) of one zygote was transferred back to the perivitelline space of the same zygote in S (S-PT; n = 216) or D (D-PT; n = 165) treatments; and two in the PT differentially activated group, where the karyoplast was removed from a zygote of one activation treatment and was then transferred to a previously enucleated zygote from the other treatment, which allowed the production of embryos with S cytoplasm and D nucleus (SCDN; n = 255) and D cytoplasm and S nucleus (DCSN; n = 279).…”

Section: Pronuclear Transfermentioning

confidence: 99%

See 1 more Smart Citation

Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle

Méo

Ferreira²,

Perecin³

et al. 2009

Animal Reproduction Science

View full text Add to dashboard Cite

Ooplasmic factors drive nuclear organization after fertilization and are also important for re-programming in nuclear transfer procedures, in which artificial activation is essential for reconstructed embryos to progress in development. The present research evaluated the effect of pronuclear transfer (PT) between zygotes parthenogenetically activated with ionomycin followed by strontium (S) or 6-DMAP (D) on early embryonic development. PT was performed in the same zygote to obtain embryos in control groups (S-PT and D-PT) and between zygotes activated with S and D to achieve embryos with differentially activated cytoplasm (C) and nucleus (N) (SCDN and DCSN). PT procedure did not affect cleavage and blastocyst rates, respectively, in PT control groups compared to non-manipulated control (S-PT: 73.6% and 7.3% compared with S-Control: 77.9% and 7.8%; and D-PT: 73.3% and 31.7% compared with D-Control: 83.1% and 41.5%). Cleavage, eight-cell, and blastocyst rates, respectively, were similar between SCDN (76.5%, 36.4%, and 6.8%) and DCSN (69.5%, 25.0%, and 4.9%) embryos. Developmental rates in SCDN were similar to S-PT, but inferior to D-PT. Developmental arrest up to eight-cell stage was greater in SCDN and DCSN than in S-PT and D-PT. In conclusion, karyoplast exchange between parthenogenetic zygotes activated with strontium and 6-DMAP can lead to nuclear-cytoplasmic incompatibilities and affect embryonic development to the eight-cell and blastocyst stages.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Pronuclear Transfermentioning

confidence: 99%