Development of immunosensor based on OWLS technique for determining Aflatoxin B1 and Ochratoxin A

Adányi, Nóra; Levkovets, I A; Rodriguez-Gil, S; Ronald, A. P.; Váradi, Mária; Szendrő, István

doi:10.1016/j.bios.2006.02.015

Cited by 134 publications

(75 citation statements)

References 37 publications

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“…Affinity-based surface plasmon resonance sensors (SPR) have the advantage of not requiring any labelling of the target mycotoxin (Tü dö s et al 2003) and may become an alternative method for rapid screening, which also enables the simultaneous detection of multiple mycotoxins using serial connected flow cells (van der Gaag et al 2003). In a further label-free immunochemical approach for the detection of aflatoxin B1 and ochratoxin A, optical waveguide lightmode spectroscopy (OWLS) was used with integrated optical waveguide sensor chips measuring the resonance incoupling angle of polarized light, thus determining the surface coverage (Adányi et al 2007).…”

Section: Immunochemical Techniquesmentioning

confidence: 99%

Mycotoxin analysis: An update

Krska¹,

Schubert-Ullrich²,

Molinelli³

et al. 2008

Food Additives & Contaminants: Part A

310

215

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Mycotoxin contamination of cereals and related products used for feed can cause intoxication, especially in farm animals. Therefore, efficient analytical tools for the qualitative and quantitative analysis of toxic fungal metabolites in feed are required. Current methods usually include an extraction step, a clean-up step to reduce or eliminate unwanted co-extracted matrix components and a separation step with suitably specific detection ability. Quantitative methods of analysis for most mycotoxins use immunoaffinity clean-up with high-performance liquid chromatography (HPLC) separation in combination with UV and/or fluorescence detection. Screening of samples contaminated with mycotoxins is frequently performed by thin layer chromatography (TLC), which yields qualitative or semi-quantitative results. Nowadays, enzyme-linked immunosorbent assays (ELISA) are often used for rapid screening. A number of promising methods, such as fluorescence polarization immunoassays, dipsticks, and even newer methods such as biosensors and non-invasive techniques based on infrared spectroscopy, have shown great potential for mycotoxin analysis. Currently, there is a strong trend towards the use of multi-mycotoxin methods for the simultaneous analysis of several of the important Fusarium mycotoxins, which is best achieved by LC-MS/MS (liquid chromatography with tandem mass spectrometry). This review focuses on recent developments in the determination of mycotoxins with a special emphasis on LC-MS/MS and emerging rapid methods.

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Section: Immunochemical Techniquesmentioning

confidence: 99%

Mycotoxin analysis: An update

Krska¹,

Schubert-Ullrich²,

Molinelli³

et al. 2008

Food Additives & Contaminants: Part A

310

215

View full text Add to dashboard Cite

show abstract

“…The results should be confirmed with the reference method (Prieto-Simon, et al, 2007;Rodrigues-Mozas,et al, 2007). It was shown in the literature that some electrochemical biosensors showed excellent sensitivity with LODs below 0.1 μg/kg (Micheli, et al, 2005;Piermarini, et al, 2007) while those based on optical detection reached LODs comparable with ELISA (0.5-10 μg/kg) (Adanyi, et al, 2007;Prieto-Simon, et al, 2007). Although traditional immunoassay based methods such as ELISA, have been applied widely to mycotoxin detection.…”

Section: Figure 2 General Schematic Representation Of Biosensorsmentioning

confidence: 65%

Application of nanotechnology in detection of mycotoxins and in agricultural sector

Sertova¹

2015

j. cent. eur. agric.

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A brief review of nanotechnology application in detection of mycotoxins and in agriculture sector was presented. Mycotoxins are secondary metabolites produced by fungi. Their toxicity is the reason for implementation of various screening methods to detect them. During the last years, the highlight was put on nanoscale materials included in biosensors, which were some of the smart devices used for determination of mycotoxins, and in agriculture sector. Over the next decade, the progress of nanotechnology will demonstrated a way to improve detection of contaminated feed and food. To achieve this purpose the innovations of nanomaterials reported every year would be applied. In the paper, some of the applications developed by nanotechnology that would contribute to the implementation of new tools for analysis of mycotoxins and agricultural products were discussed.Keywords: agriculture, biosensors, mycotoxins, nanoparticles, nanotechnology Резюме Представен е кратък преглед за приложение на нанотехнологиите за откриване на микотоксини, както и в аграрния сектор. Микотоксините са вторични метаболити, произвеждани от гъбички. Тяхната токсичност е причина за прилагане на различни методи за откриването им. През последните години акцента е бил поставен върху нано материали, вградени в биосензори, които са част от модерните устройства, използвани за откриване на микотоксини, а също и в аграрния сектор. През следващото десетилетие ще бъде доказван напредъка на нанотехнологиите да бъдат използвани като метод за детекция на замърсени фуражи и храни. За постигането на тази цел всяка година ще се следят новостите при наноматериалите. В статията са обсъдени някои от приложенията,разработени с помощта на нанотехнологиите, които биха допринесли за изграждането на нови устройства за анализ на микотоксини и селскостопански продукти.

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“…Array sensor is a good tool for multiple compounds. For determination of aflatoxin B1 and ochratoxin A in the same operation, Adányi et al (Adányi et al, 2007) devised a solid-array sensor with a sensitive detection range of 0.5-10 ng mL-1 using a competitive detection method.…”

Section: Biosensormentioning

confidence: 99%

Aflatoxin Measurement and Analysis

Hussain¹

2011

Aflatoxins - Detection, Measurement and Control

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Development of immunosensor based on OWLS technique for determining Aflatoxin B1 and Ochratoxin A

Cited by 134 publications

References 37 publications

Mycotoxin analysis: An update

Mycotoxin analysis: An update

Application of nanotechnology in detection of mycotoxins and in agricultural sector

Aflatoxin Measurement and Analysis

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