Development of immunochromatographic test systems for express detection of plant viruses

Byzova, Nadezhda A.; Safenkova, Irina V.; Chirkov, Sergei; Zherdev, Anatoly V.; Blintsov, A. N.; Dzantiev, B.G.; Атабеков, И.Г.

doi:10.1134/s000368380902015x

Cited by 33 publications

(10 citation statements)

References 9 publications

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“…Another interesting improvement in serological methods was the development of immunostrips for the early and rapid detection of viral infections in plants. In this context, Byzova et al [ 71 ] were instrumental in developing an immunochromatographic strip-based assay for the possible detection of five plant viruses (Bean mild mosaic virus, Spherical carnation mottle virus, Potato viruses X and Y, and Tobacco mosaic virus) inleaf extracts of virus-infected plants (sensitivity range 0.08–0.5 µg/mL for 10 min). The authors used multi membrane-based composites immobilized with polyclonal antibodies against the gold-conjugated antibodies and viruses in the developed assays.…”

Section: Methods For Plant Viral Diagnosticmentioning

confidence: 99%

Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review

Mehetre

Leo

Singh³

et al. 2021

Viruses

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Plant viral diseases are the foremost threat to sustainable agriculture, leading to several billion dollars in losses every year. Many viruses infecting several crops have been described in the literature; however, new infectious viruses are emerging frequently through outbreaks. For the effective treatment and prevention of viral diseases, there is great demand for new techniques that can provide accurate identification on the causative agents. With the advancements in biochemical and molecular biology techniques, several diagnostic methods with improved sensitivity and specificity for the detection of prevalent and/or unknown plant viruses are being continuously developed. Currently, serological and nucleic acid methods are the most widely used for plant viral diagnosis. Nucleic acid-based techniques that amplify target DNA/RNA have been evolved with many variants. However, there is growing interest in developing techniques that can be based in real-time and thus facilitate in-field diagnosis. Next-generation sequencing (NGS)-based innovative methods have shown great potential to detect multiple viruses simultaneously; however, such techniques are in the preliminary stages in plant viral disease diagnostics. This review discusses the recent progress in the use of NGS-based techniques for the detection, diagnosis, and identification of plant viral diseases. New portable devices and technologies that could provide real-time analyses in a relatively short period of time are prime important for in-field diagnostics. Current development and application of such tools and techniques along with their potential limitations in plant virology are likewise discussed in detail.

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Section: Methods For Plant Viral Diagnosticmentioning

confidence: 99%

Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review

Mehetre

Leo

Singh³

et al. 2021

Viruses

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“…Conjugate of gold nanoparticles (GNP) with mouse monoclonal antibodies specific to fluorescein (anti-FAM) (clone 2A3c, Bialexa, Russia) and LFA test strips were prepared as described at [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

“…Test strips were assembled using the sample membrane GFB-R4, final adsorbing membrane AP045 (Advanced Microdevices), and the aforementioned fiberglass and nitrocellulose membranes. The multimembrane composites were cut and packed according to Byzova et al [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

The Challenge for Rapid Detection of High-Structured Circular RNA: Assay of Potato Spindle Tuber Viroid Based on Recombinase Polymerase Amplification and Lateral Flow Tests

Иванов

Shmyglya²,

Zherdev

et al. 2020

Plants

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An assay was developed to detect the potato spindle tuber viroid (PSTVd), a dangerous plant pathogen that causes crop damage resulting in economic losses in the potato agriculture sector. The assay was based on the reverse transcription and recombinase polymerase amplification (RT-RPA) of PSTVd RNA coupled with amplicon detection via lateral flow assay (LFA). Primers labeled with fluorescein and biotin were designed for RT-RPA for effective recognition of the loop regions in the high-structured circular RNA of PSTVd. The labeled DNA amplicon was detected using lateral flow test strips consisting of a conjugate of gold nanoparticles with antibodies specific to fluorescein and streptavidin in the test zone. The RT-RPA-LFA detected 106 copies of in vitro transcribed PSTVd RNA in reaction or up to 1:107 diluted extracts of infected plant leaves. The assay took 30 min, including the RT-RPA stage and the LFA stage. The testing of healthy and infected potato samples showed full concordance between the developed RT-RPA-LFA and quantitative reverse transcription polymerase chain reaction (RT-qPCR) and the commercial kit. The obtained results proved the feasibility of using the developed assay to detect PSTVd from a natural source.

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“…Physical adsorption was used for the synthesis of GNP–antibody conjugates [43]. To determine the number of biomolecules for the synthesis of conjugates, the minimum stabilizing concentration was determined using the flocculation curve.…”

Section: Methodsmentioning

confidence: 99%

Enlargement of Gold Nanoparticles for Sensitive Immunochromatographic Diagnostics of Potato Brown Rot

Razo

Panferova

Panferov

et al. 2019

Sensors

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Lateral flow immunoassay (LFIA) is a convenient tool for rapid field-based control of various bacterial targets. However, for many applications, the detection limits obtained by LFIA are not sufficient. In this paper, we propose enlarging gold nanoparticles’ (GNPs) size to develop a sensitive lateral flow immunoassay to detect Ralstonia solanacearum. This bacterium is a quarantine organism that causes potato brown rot. We fabricated lateral flow test strips using gold nanoparticles (17.4 ± 1.0 nm) as a label and their conjugates with antibodies specific to R. solanacearum. We proposed a signal enhancement in the test strips’ test zone due to the tetrachloroauric (III) anion reduction on the GNP surface, and the increase in size of the gold nanoparticles on the test strips was approximately up to 100 nm, as confirmed by scanning electron microscopy. Overall, the gold enhancement approach decreased the detection limit of R. solanacearum by 33 times, to as low as 3 × 104 cells∙mL–1 in the potato tuber extract. The achieved detection limit allows the diagnosis of latent infection in potato tubers. The developed approach based on gold enhancement does not complicate analyses and requires only 3 min. The developed assay together with the sample preparation and gold enlargement requires 15 min. Thus, the developed approach is promising for the development of lateral flow test strips and their subsequent introduction into diagnostic practice.

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Development of immunochromatographic test systems for express detection of plant viruses

Cited by 33 publications

References 9 publications

Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review

Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review

The Challenge for Rapid Detection of High-Structured Circular RNA: Assay of Potato Spindle Tuber Viroid Based on Recombinase Polymerase Amplification and Lateral Flow Tests

Enlargement of Gold Nanoparticles for Sensitive Immunochromatographic Diagnostics of Potato Brown Rot

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