Development of immunization trials against Acinetobacter baumannii

Ahmad, Tarek A.; Tawfik, Dina M.; Sheweita, Salah A.; Haroun, Medhat A.; El-Sayed, L.

doi:10.1016/j.trivac.2016.03.001

Cited by 55 publications

(32 citation statements)

References 124 publications

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“…The interaction occurring between OMPs and Fn might represent a critical step for lung epithelial colonization in A. baumannii mediated infections [97]. OmpA (previously known as Omp38) is a highly conserved OMP in clinical isolates and is one of the best-characterized virulence factors of A. baumannii [100][101][102]. Expression of ompA is associated with the cytotoxicity to eukaryotic cells and the adhesion to Fn [42,103].…”

Section: Acinetobacter Baumanniimentioning

confidence: 99%

Interaction with the host: the role of fibronectin and extracellular matrix proteins in the adhesion of Gram-negative bacteria

Vaca

Thibau

Schütz

et al. 2019

Med Microbiol Immunol

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The capacity of pathogenic microorganisms to adhere to host cells and avoid clearance by the host immune system is the initial and most decisive step leading to infections. Bacteria have developed different strategies to attach to diverse host surface structures. One important strategy is the adhesion to extracellular matrix (ECM) proteins (e.g., collagen, fibronectin, laminin) that are highly abundant in connective tissue and basement membranes. Gram-negative bacteria express variable outer membrane proteins (adhesins) to attach to the host and to initiate the process of infection. Understanding the underlying molecular mechanisms of bacterial adhesion is a prerequisite for targeting this interaction by "anti-ligands" to prevent colonization or infection of the host. Future development of such "anti-ligands" (specifically interfering with bacteria-host matrix interactions) might result in the development of a new class of anti-infective drugs for the therapy of infections caused by multidrug-resistant Gram-negative bacteria. This review summarizes our current knowledge about the manifold interactions of adhesins expressed by Gram-negative bacteria with ECM proteins and the use of this information for the generation of novel therapeutic antivirulence strategies.

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Section: Acinetobacter Baumanniimentioning

confidence: 99%

Interaction with the host: the role of fibronectin and extracellular matrix proteins in the adhesion of Gram-negative bacteria

Vaca

Thibau

Schütz

et al. 2019

Med Microbiol Immunol

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“…These implications highlight active and passive immunizations as cost‐effective approaches to reduce the clinical and economic burden of infections caused by this notorious pathogen (Ahmad et al . ). Hence, specific antibodies could be considered one of the most promising strategies to prevent and treat infections caused by the bacterium (Cohen et al .…”

Section: Introductionmentioning

confidence: 97%

Specific egg yolk antibodies (IgY) confer protection againstAcinetobacter baumanniiin a murine pneumonia model

et al. 2018

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Aim: Acinetobacter baumannii, an increasingly serious health threat, is considered as one of the six most dangerous microbes of high mortality rate. However, treatment of its infections is difficult because of the lack of efficient antibiotic or commercial vaccines. Passive immunization through administration of specific antibodies such as IgY, could be an attractive practical solution. Methods and Results: In the current study, antigenicity of two recombinant outer membrane proteins (OmpA and Omp34) as well as inactivated whole cell of A. baumannii was assessed by ELISA. Moreover, prophylactic effects of specific IgY antibodies (avian antibody) raised against these antigens were evaluated in a murine pneumonia model. The specific IgY antibodies had various prophylactic effects in the pneumonia model. OmpA was the most potent antigen in terms of triggering antibody and conferring protection. While a synergic effect was observed in ELISA for antibodies raised against a combination of OmpA and Omp34 (which are known as Omp33-36 and Omp34 kDa), an antagonistic effect was unexpectedly seen in challenges. The reason for this phenomenon remains to be precisely addressed. Conclusion: All the specific IgY antibodies could protect mice against pneumonia caused by A. baumannii. Significance and Impact of the Study: The specific IgY antibodies could be employed as a pharmaceutical against pneumonia caused by A. baumannii. emergence of highly antibiotic-resistant strains including multidrug-resistant (MDR) and pan-drug-resistant strains (Wang et al. 2003;Dijkshoorn et al. 2007;Pach on and McConnell 2014). In spite of the increasing prevalence of MDR strains and their high mortality, an efficient antibiotic is not provided by the pharmaceutical industry for the treatment of its infections (Pach on and McConnell 2014). These implications highlight active and passive immunizations as cost-effective approaches to reduce the clinical and economic burden of infections caused by this notorious pathogen (Ahmad et al. 2016).

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“…Active and passive immunization with IWCs, OMVs and OMCs demonstrated protection of mice from bacterial challenge. However, owing to their complex composition and potential safety concerns, neither of them is the ideal vaccine candidate . Subunit vaccines based on single recombinant protein or chimeric proteins have been evaluated for their antigenicity and protective efficacy in the past few years, but to date no A baumannii vaccine has entered phase I clinical trial .…”

Section: Discussionmentioning

confidence: 99%

MF59 adjuvant enhances the immunogenicity and protective immunity of the OmpK/Omp22 fusion protein from Acineterbacter baumannii through intratracheal inoculation in mice

Yang

SanJun

et al. 2019

Scand J Immunol

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Acinetobacter baumannii (A baumannii) is an emerging nosocomial pathogenic bacterium which leads to hospital infections. The increase in drug-resistant A baumannii strains makes it difficult to control by using common antibiotics. The development of effective vaccines is an alternative means to avoid A baumannii infections. In the present study, Balb/c mice were inoculated intratracheally with 30 μg of OmpK/

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Development of immunization trials against Acinetobacter baumannii

Cited by 55 publications

References 124 publications

Interaction with the host: the role of fibronectin and extracellular matrix proteins in the adhesion of Gram-negative bacteria

Interaction with the host: the role of fibronectin and extracellular matrix proteins in the adhesion of Gram-negative bacteria

Specific egg yolk antibodies (IgY) confer protection againstAcinetobacter baumanniiin a murine pneumonia model

MF59 adjuvant enhances the immunogenicity and protective immunity of the OmpK/Omp22 fusion protein from Acineterbacter baumannii through intratracheal inoculation in mice

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