Development of immunity to incoming radiolabelled larvae in lambs continuously infected with Ostertagia circumcincta

Seaton, D.S.; Jackson, F.; Smith, W.D.; Angus, K.W.

doi:10.1016/s0034-5288(18)31152-4

Cited by 76 publications

(38 citation statements)

References 21 publications

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“…Mascot scores above 50 with two peptides matched were considered significant. associated with previously immune tissue than previously naïve tissue, is in agreement with previous studies, where both in vitro tissue association [13] and in vivo larval establishment in naïve tissue were shown to be between 30-60% [27]. The present study showed that the tissue explants can maintain immunological function in vitro, at least for the time tested in this study.…”

Section: Discussionsupporting

confidence: 93%

Proteomic approach to identify candidate effector molecules during the in vitro immune exclusion of infectiveTeladorsagia circumcinctain the abomasum of sheep

Athanasiadou

Pemberton²,

Jackson³

et al. 2008

Vet. Res.

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-In the present study we have employed an in vitro organ challenge model to study the post-challenge responses in parasite naïve and immune gastric tissue of sheep, in an attempt to identify the host derived factors involved in immune exclusion of Teladorsagia circumcincta larvae. Proteins present in the epithelial cells and mucus from ovine abomasa following parasite challenge in previously naïve and immune animals were analysed through Matrix Assisted Laser Desorption/Ionization-Time of Flight (MALDI-Tof)-MS and shotgun proteomics. MALDI-ToF analysis of epithelial cell lysates revealed that a number of proteins identified were differentially expressed in naïve and immune cells. These included intelectin and lysozymes, which were present at higher levels in epithelial cell lysates derived from immune samples. A large number of proteins were identified in the mucosal wash from immune tissue which were not present in the mucosal wash of the naïve tissue. Some of these proteins were present in washes of immune tissue prior to the parasite challenge including immunoglobulin A, galectin 14 and 15 and sheep mast cell protease 1. However, other proteins, such as calcium activated chloride channel and intelectin were only detected in the washings from the challenged tissue. The latter may be related to an enhanced mucus release, which may result in entrapment of infective larvae and thus reduced establishment in tissue that has been previously challenged with the parasite. In conclusion, several proteins have been identified which may be involved, either directly or indirectly, in the exclusion and immune elimination of incoming infective larvae. In the present study, the usefulness of the in vitro model has been confirmed, and the global proteomic approach has identified proteins that had not previously been associated with parasite exclusion from abomasal mucosa, such as the calcium activated chloride channel.immunity / mucus / proteomics / sheep / Teladorsagia circumcincta

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Section: Discussionsupporting

confidence: 93%

Proteomic approach to identify candidate effector molecules during the in vitro immune exclusion of infectiveTeladorsagia circumcinctain the abomasum of sheep

Athanasiadou

Pemberton²,

Jackson³

et al. 2008

Vet. Res.

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“…The number of globule leukocytes was negatively correlated with the number of T. circumcincta and T. colubriformis worms, suggesting that this cell type plays a role in the resistance of the host [2]. This is in agreement with the results of Seaton et al [12] and Stear et al [14] for T. circumcincta in lambs more than 6 months old following experimental infections, and Douch et al [4] for T. colubriformis, in the case of natural infections. Globule leukocyte counts were also negatively correlated to worm egg production and the number of eggs in utero, but only for T. circumcincta.…”

Section: Discussionsupporting

confidence: 89%

Regulation of Teladorsagia circumcincta and Trichostrongylus colubriformis worm populations by grazing sheep with differing resistance status

Gruner¹,

Cortet²,

Sauvé³

et al. 2004

Vet. Res.

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-In an experiment lasting 4 years, changes in the Teladorsagia circumcincta andTrichostrongylus colubriformis populations were compared in lambs and adult sheep with differing resistance statuses. Two flocks of 30 rams (resistant R and susceptible S) grazed separate pastures and 8 rams were slaughtered in the middle and at the end of each grazing season. Five groups of tracer lambs were added each year to estimate the pasture infectivity and were killed for worm counts. The availability of animals with differing resistance statuses (rams and tracer lambs) and differing levels of infection made it possible to investigate the number, size and fecundity of worms of these two species. The inflammatory response was measured in the rams by counting the globule leukocytes, mast cells and eosinophils in the fundic, pyloric and intestinal mucosa. In the tracer lambs, the daily egg production by the female worms of both species was negatively correlated with the worm burden. Worm length accounted for 60 and 70% of the variation in the number of eggs in utero for T. circumcincta and T. colubriformis respectively. Worm length was closely associated with the resistance status of the host; there were greater differences between lambs, and S and R rams for T. colubriformis. T. circumcincta worm lengths were not affected by the worm number. Globule leukocyte counts were related to the worm burdens, and mast cell counts to worm length in the R and S rams. The number, size and fecundity of the worms may well be regulated by similar mechanisms in both species, but T. colubriformis seemed to be more intensively regulated than T. circumcincta. This finding could be useful in devising more effective methods of parasite control.sheep-nematoda / Teladorsagia circumcincta / Trichostrongylus colubriformis / population dynamics / resistance

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“…The relative low FEC observed herein is probably associated with the immune status of the lambs prior to the start of the trickle infection. The response of these lambs to the secondary infection seems to correlate with earlier findings in re-infected lambs (Seaton et al, 1989). However, the breeds used in the present study were likely to differ in their ability to express their acquired immunity to infection, if we consider the occurrence of anorexia and the high plasma leptin concentrations in the INF2 S lambs but not in the INF2 B.…”

Section: Discussionsupporting

confidence: 83%

Effects of nematode infection on anorexia and leptin levels in lambs of two breeds

Zaralis¹,

Tolkamp²,

Houdijk³

et al. 2007

J. Anim. Feed Sci.

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The objective of this study was to investigate whether breed differences in production potential are associated with differences in the occurrence of anorexia following a nematode infection and whether plasma leptin concentrations elevate as a result of infection. Weaned lambs, of the Suffolk × Greyface cross and Scottish Blackface breed were either trickle infected with 21,000 infective Teladorsagia circumcincta larvae per week, or not infected. The results suggest that both the magnitude and duration of anorexia differ considerably between the two breeds and is likely to associate with their differences in immune response. In addition, results suggest that leptin has a role in the expression of immunity and parasite-induced anorexia in sheep.

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Development of immunity to incoming radiolabelled larvae in lambs continuously infected with Ostertagia circumcincta

Cited by 76 publications

References 21 publications

Proteomic approach to identify candidate effector molecules during the in vitro immune exclusion of infectiveTeladorsagia circumcinctain the abomasum of sheep

Proteomic approach to identify candidate effector molecules during the in vitro immune exclusion of infectiveTeladorsagia circumcinctain the abomasum of sheep

Regulation of Teladorsagia circumcincta and Trichostrongylus colubriformis worm populations by grazing sheep with differing resistance status

Effects of nematode infection on anorexia and leptin levels in lambs of two breeds

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