Development of imipenem resistance in an Aeromonas veronii biovar sobria clinical isolate recovered from a patient with cholangitis

Sánchez‐Céspedes, Javier; Figueras, María José; Aspíroz, Carmen; Aldea, María José; Toledo, Miguel Áquila; Alperí, Anabel; Marco, Francesc; Vila, Jordi

doi:10.1099/jmm.0.47804-0

Cited by 50 publications

(29 citation statements)

References 28 publications

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“…To our knowledge, a fatal case of acquired carbapenemresistant Aeromonas septicemia has not yet been reported. A case report on hepatobiliary tract infection with carbapenem-resistant A. veronii biovar sobria, due to the induction of chromosomal carbapenemase, was first reported in 2009 (6). Verona integrin-encoded metallob-lactamase-producing Aeromonas was also isolated in a surveillance study conducted in Israel (8).…”

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The Rapid Induction of Carbapenem-Resistance in an <i>Aeromonas dhakensis</i> Blood Isolate

et al. 2016

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SUMMARY: Meropenem-susceptible and -resistant Aeromonas dhakensis isolates from blood cultures of a fatal case of septicemia were analyzed. The two isolates were homologous and gene expression of metallo-b-lactamase in the resistant strain was upregulated. Physicians should be aware of the possibility of the induction of carbapenem-resistance, following the use of carbapenems in the treatment of Aeromonas infection.In an earlier study, conducted at a hospital in Japan, we observed that Aeromonas septicemia occurred frequently in hospitalized patients with underlying diseases, including malignancy (1). In this report, a fatal case of Aeromonas septicemia in a girl with neutropenia is described. Two strains of Aeromonas veronii biovar sobria were isolated from blood cultures obtained before and after treatment with carbapenem. The strain isolated at the onset of sepsis was sensitive to carbapenem; however, the strain isolated after treatment, was resistant. Aeromonas species have chromosomal genes, such as cphA and imiS, encoding metallo-b-carbapenemase, which are not commonly expressed, but inducible. These genes have similar sequences and the enzymes can hydrolyze carbapenems efficiently (2,3). To understand the mechanism underlying the development of resistance to carbapenem, we analyzed the genetic homology between the blood isolates and the gene expression levels of the chromosomal metallo-blactamase.The two Aeromonas strains (meropenem-susceptible, MIC 0.5 mg/mL; meropenem-resistant, MIC

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“…were quantified using the LightCycler480 (Roche Diagnostics, Tokyo, Japan). The gene expression level of cphA/imiS was corrected using the expression level of 16SrRNA (6). Compared with the meropenem-susceptible strain, the expression of metallo-b-lactamase in the meropenem-resistant strain was upregulated by 13.5-fold (n ＝ 3).…”

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The Rapid Induction of Carbapenem-Resistance in an <i>Aeromonas dhakensis</i> Blood Isolate

et al. 2016

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“…A 2009 study of Aeromonas in France recommended that a cephalosporin or a quinolone be prescribed along with an aminoglycoside in cases of severe infection (6). We believe that the reported emerging resistance of Aeromonas to quinolones (1,10) and carbapenems (7,11), as well as aztreonam, cephalosporins, and aminoglycosides (this report), might influence physicians to employ polytherapy for empirical treatment of severe Aeromonas hydrophila infections. However, comprehensive studies are needed to examine broader trends in Aeromonas antibiotic resistance.…”

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Aminoglycoside-Resistant Aeromonas hydrophila as Part of a Polymicrobial Infection following a Traumatic Fall into Freshwater

et al. 2011

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Amikacin is a first-line treatment for Aeromonas infection due to high efficacy. There are few reports of aminoglycoside-resistant Aeromonas spp. We report a soft tissue infection containing multiple pathogens, including a strain of Aeromonas hydrophila resistant to amikacin, tobramycin, and multiple cephalosporins. CASE REPORTA 35-year-old man presented to the emergency department of our academic tertiary care facility after suffering a right posterior leg laceration. He was walking on a wooden dock over the Chattahoochee River in Georgia when the structure collapsed beneath him. A wooden shard or a metal pipe underneath the dock lacerated his leg before he fell into the river, and the wound was exposed to water. Shortly after the fall, a physician friend cleaned the wound with povidone-iodine and sutured the laceration closed. He also received a tetanus booster and cephalexin at a local hospital. Past medical and surgical histories were unremarkable.

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“…It can cause wound infections, diarrhea, or septicaemia in immunocompromised patients (4,6,10). A. veronii is also the causative agent of bacterial hemorrhagic septicemia in fish and is becoming a major economic problem in the fish-farming industry (2).…”

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Complete Genome Sequence of Aeromonas veronii Strain B565

Liu

Zhou

et al. 2011

J Bacteriol

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Aeromonas veronii strain B565 was isolated from aquaculture pond sediment in China. We present here the complete genome sequence of B565 and compare it with 2 published genome sequences of pathogenic strains in the Aeromonas genus. The result represents an independent stepwise acquisition of virulence factors of pathogenic strains in this genus.Aeromonas veronii is a Gram-negative, rod-shaped bacterium, commonly isolated from clinical, environmental, and food samples (1). It can cause wound infections, diarrhea, or septicaemia in immunocompromised patients (4,6,10). A. veronii is also the causative agent of bacterial hemorrhagic septicemia in fish and is becoming a major economic problem in the fish-farming industry (2). A. veronii strain B565, isolated from aquaculture pond sediment in Tianjin, China, was found to have the ability to produce chitinase to control fungal or Myxozoa-related diseases in a separate study.Whole-genome sequencing of A. veronii B565 was performed with a combined strategy involving Roche/454 (8) and Solexa paired-end sequencing technology (3). Genomic libraries containing 5.8-kb inserts were constructed, and 225,134 paired-end reads were generated using the GS FLX system, giving 18.8-fold coverage of the genome. A total of 94.65% of the reads were assembled into 3 large scaffolds, including 62 nonredundant contigs, using a 454 Newbler assembler (454 Life Science, Branford, CT). A total of 4,340,032 reads (2-kb insert) were generated to reach a depth of 95-fold coverage with an Illumina Solexa GA IIx and mapped to the scaffolds using a Burrows-Wheeler alignment (BWA) tool (7). The interscaffold and intrascaffold gaps were filled by local assembly of Roche and Solexa reads around or sequencing PCR products using an ABI 3730 capillary sequencer. The analysis of the genome was performed as described previously (5, 13).The complete genome sequence of B565 contains a circular 4,551,783-bp chromosome, with a GC content of 58.72%. There are 4,057 protein-coding genes, 10 rRNA operons, and 102 tRNA genes in its genome. B565 encodes some putative virulence factors, such as hemolysins, RTX protein, adhesion factor, flagella, and mannose-sensitive hemagglutinin (MSHA) (12), all of which were shared with at least one of the sequenced genomes for Aeromonas hydrophila ATCC 7966 (11) and A. salmonicida A449 (9). There are 5 genes encoding chitinase in B565, and all could be found in ATCC 7966 and A449, indicating an important role for these conserved chitinases in Aeromonas.There are fewer virulence genes in B565 than in ATCC 7966 and A449, suggesting a less virulent or nonvirulent character. There are 346 genes shared by ATCC 7966 and A449 but absent in B565. Some of these genes encode putative virulence factors such as hemolysins and the type III secretion protein. There are 329 and 666 unique genes in ATCC 7966 and A449, respectively, some of which are virulence genes and often form large clusters, such as the rtx cluster in ATCC 7966 and the flagellar gene cluster in A449, or are related to mobile ...

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Development of imipenem resistance in an Aeromonas veronii biovar sobria clinical isolate recovered from a patient with cholangitis

Cited by 50 publications

References 28 publications

The Rapid Induction of Carbapenem-Resistance in an <i>Aeromonas dhakensis</i> Blood Isolate

The Rapid Induction of Carbapenem-Resistance in an <i>Aeromonas dhakensis</i> Blood Isolate

Aminoglycoside-Resistant Aeromonas hydrophila as Part of a Polymicrobial Infection following a Traumatic Fall into Freshwater

Complete Genome Sequence of Aeromonas veronii Strain B565

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