Development of human minor salivary glands: expression of mucins according to stage of morphogenesis

Teshima, Tathyane Harumi Nakajima; Ianez, Renata F; Coutinho‐Camillo, Cláudia Malheiros; Buim, Marcilei Eliza Cavicchioli; Soares, Fernando Augusto; Lourenço, Sílvia Vanessa

doi:10.1111/j.1469-7580.2011.01405.x

Cited by 20 publications

(19 citation statements)

References 36 publications

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“…Although the murine and human glands differ in gross morphology, they undergo similar stages of morphogenesis (Teshima et al, 2011) and are highly innervated (Figure 6A and Figure 6—figure supplement 1A). Due to the paucity of expression data on human fetal salivary gland, we first characterized expression of acinar and duct markers as well as the location of CHRM1, SOX10 and SOX2.…”

Section: Resultsmentioning

confidence: 99%

SOX2 regulates acinar cell development in the salivary gland

Emmerson

May

Nathan

et al. 2017

eLife

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Acinar cells play an essential role in the secretory function of exocrine organs. Despite this requirement, how acinar cells are generated during organogenesis is unclear. Using the acini-ductal network of the developing human and murine salivary gland, we demonstrate an unexpected role for SOX2 and parasympathetic nerves in generating the acinar lineage that has broad implications for epithelial morphogenesis. Despite SOX2 being expressed by progenitors that give rise to both acinar and duct cells, genetic ablation of SOX2 results in a failure to establish acini but not ducts. Furthermore, we show that SOX2 targets acinar-specific genes and is essential for the survival of acinar but not ductal cells. Finally, we illustrate an unexpected and novel role for peripheral nerves in the creation of acini throughout development via regulation of SOX2. Thus, SOX2 is a master regulator of the acinar cell lineage essential to the establishment of a functional organ.DOI: http://dx.doi.org/10.7554/eLife.26620.001

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Section: Resultsmentioning

confidence: 99%

SOX2 regulates acinar cell development in the salivary gland

Emmerson

May

Nathan

et al. 2017

eLife

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“…The developmental pattern of human SGs has already been described as following a similar process compared to the mouse (Lourenço, Uyekita, Lima, & Soares, 2008;Teshima et al, 2011), although little is known about the role of apoptosis during human glandular morphogenesis. The expression of apoptosis-related proteins analysed in this study support a role for programmed cell death in gland development, particularly during lumen formation agreeing with recent research from the mouse (Teshima et al, 2016), however in the human samples apoptosis did not appear to involve the cleavage of the well-known downstream apoptotic regulator caspase-3.…”

Section: Discussionmentioning

confidence: 99%

“…SG development has classically been divided into five embryonic stages, well described as prebud, initial bud, pseudoglandular, canalicular (or cavitation), and terminal bud, observed in both mouse and human development (Melnick & Jaskoll, 2000;Teshima et al, 2011;Tucker, 2007). Apoptosis is a type of programmed cell death, which has been shown to be very important for the regulation of developmental and pathological processes (Elmore, 2007).…”

Section: Introductionmentioning

confidence: 99%

Apoptosis-associated protein expression in human salivary gland morphogenesis

Teshima

Ianez

Coutinho‐Camillo

et al. 2016

Archives of Oral Biology

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Objective: Salivary gland (SG) development is based on branching morphogenesis, in which programmed cell death has been proposed to play a role in cell signalling and organ shaping.In the mouse salivary gland apoptosis has been suggested to play a key role in lumen formation, removing the central cells of the epithelial stalks. Here we analyse the expression of several anti-and pro-regulators of apoptosis during human SG development in a range of developmental stages.Design: Foetal SGs obtained from the University of São Paulo were analysed by immunohistochemistry to assess the expression of apoptosis-associated proteins: caspases (caspase-6, -7, -9 and cleaved caspase-3), Bcl-2 family members (Bax, Bak, Bad, Bid, Bcl-2, Bcl-x and Bcl-xL), Survivin (BIRC5), Cytochrome C and Apaf-1.Results: Nuclear expression of Bax and Bak was identified in presumptive luminal areas at initial stages, while Bcl-xL showed the most relevant anti-apoptotic activity. Caspase-6, -7 and -9 were expressed during all stages, while interestingly cleaved caspase-3 showed no prominent expression, indicating that caspase-7 is the main effector. Apoptosome complex components Apaf-1 and Cytochrome C, as well as survivin were all positive in developing glands. Conclusions:The particular expression pattern of several apoptotic regulators in human SG development suggests the existence of a fundamental role for apoptosis during duct formation. The absence of Bad and Bid expressions indicates that the instrinsic pathway is more active then the extrinsic during human gland formation. The subcellular localisation of intrinsic-apoptosis proteins correlated with apoptotic activity, but also suggested additional non-apoptotic functions. Highlights: Caspase-7 is the main executioner caspase involved in human SG duct development  Consistent cytoplasmic expression suggest additional non-apoptotic functions  Intrinsic-type apoptotic pathway drives human SG development

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“…2d, Table 5). According to Liu et al (33), MUC-1 may facilitate the secretion flow through the ductal system, and it is believed that this function occurs because of decreased cell-cell interactions via the cellular apical domain (33)(34)(35). Kirkeby et al studied the MUC-1 and mucin-type O-glycan expression in adult human submandibular glands, confirmed ductal presence for MUC-1, and laid emphasis on the role of this molecule in tissue physiology (36).…”

Section: Discussionmentioning

confidence: 99%

Glycomic profile of the human parotid gland between 18th and 26th week of fetal development

Rego¹,

Filho²,

Sobral

et al. 2016

J Oral Sci

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The formation of new and functional structural components of several organs, such as parotid glands, can be influenced by the glycocode. This study analyzed the glycobiology of parotid salivary gland tissue during fetal development using specific biochemical probes (lectins and antibodies). Eleven parotid gland samples from human fetuses were obtained from spontaneous abortions at 14-28 weeks of gestation, and tissue sections were analyzed for lectin histochemistry and immunohistochemistry. From the 18th to 26th week, Canavalia ensiformis agglutinin, wheat germ agglutinin, Ulex europaeus agglutinin-I, peanut agglutinin, Sambucus nigra agglutinin, and Vicia villosa agglutinin lectin staining were predominantly observed in the apical and/or basement membranes of the ducts and tubulo-acinar units. Moreover, the presence of galectin-1 was found in the membrane, cytoplasm, and nucleus of both structures. Conversely, Gal-3 and mucin-1 were restricted to the glandular ducts. The lectin staining pattern changed during the weeks evaluated. Nevertheless, the carbohydrate subcellular localization represented a key factor in the investigation of structural distribution profiles and possible roles of these glycans in initial parotid gland development.

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Development of human minor salivary glands: expression of mucins according to stage of morphogenesis

Cited by 20 publications

References 36 publications

SOX2 regulates acinar cell development in the salivary gland

SOX2 regulates acinar cell development in the salivary gland

Apoptosis-associated protein expression in human salivary gland morphogenesis

Glycomic profile of the human parotid gland between 18th and 26th week of fetal development

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