Development of human corneal epithelium on organized fibrillated transparent collagen matrices synthesized at high concentration

Tidu, Aurélien; Ghoubay-Benallaoua, Djida; Lynch, Barbara; Haye, Bernard; Illoul, Corinne; Allain, Jean-Marc; Borderie, Vincent; Mosser, Gervaise

doi:10.1016/j.actbio.2015.04.018

Cited by 31 publications

(43 citation statements)

References 38 publications

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“…This strategy has been widely used to induce fibrillogenesis in concentrated type I collagen solutions at room temperature. 32,33 In our case, using ammonia vapors presents another key advantage: it lowers the freezing temperature of ice.…”

Section: Scaffold Structuration and Propertiesmentioning

confidence: 88%

Topotactic Fibrillogenesis of Freeze-Cast Microridged Collagen Scaffolds for 3D Cell Culture

Rieu

Parisi

Mosser

et al. 2019

ACS Appl. Mater. Interfaces

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Type I collagen is the main component of the extra-cellular matrix (ECM). In vitro, under a narrow window of physico-chemical conditions, type I collagen self-assembles to form complex supramolecular architectures reminiscent of those found in native ECM. Presently, a major challenge in collagen-based biomaterials is to couple the delicate collagen fibrillogenesis events with a controlled shaping process in non-denaturating conditions. In this work an ice-templating approach promoting the structuration of collagen into macroporous monoliths is used. Instead of common solvent removal procedures, a new topotactic conversion approach yielding self-assembled ordered fibrous materials is implemented. These collagen-only, non-cross-linked scaffolds exhibit uncommon mechanical properties in the wet state. With the help of the ice-patterned micro-ridge features, Normal Human Dermal Fibroblasts and C2C12 murine myoblasts successfully migrate and form highly-aligned populations within the resulting 3D biomimetic collagen scaffolds. These results open a new pathway to the development of new tissue engineering scaffolds ordered across various organization levels from the molecule to the macropore, and are of particular interest for biomedical applications where large scale 3D cell alignment is needed such as for muscular or nerve reconstruction.

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Section: Scaffold Structuration and Propertiesmentioning

confidence: 88%

Topotactic Fibrillogenesis of Freeze-Cast Microridged Collagen Scaffolds for 3D Cell Culture

Rieu

Parisi

Mosser

et al. 2019

ACS Appl. Mater. Interfaces

Self Cite

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“…As type I collagen concentrations gradually increase during vitrification of gels, collagen molecules self‐assemble and eventually reach a nematic, liquid crystal‐like structure . Incorporation of CD into the collagen gelation and vitrification process interfered with the self‐assembly process ( Figure a).…”

Section: Resultsmentioning

confidence: 99%

“…Without the native corneal structure, it is challenging to simultaneously achieve both the mechanical properties adequate for suturing and the transparency for vision. Methods to concentrate collagen, including vitrification, can produce orthogonal lamellae similar to the native tissue but the materials do not reach the thickness of the human cornea relevant for clinical transplantation. While these materials may be useful as membranes for delivering cells, an off‐the‐shelf, acellular cornea substitute that stimulates endogenous cells to reconstruct cornea tissues provides an alternative to cadaveric allotransplantation and injury repair.…”

Section: Introductionmentioning

confidence: 99%

Cyclodextrin Modulated Type I Collagen Self‐Assembly to Engineer Biomimetic Cornea Implants

Majumdar

Wang

Sommerfeld

et al. 2018

Adv Funct Materials

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Collagen-rich tissues in the cornea exhibit unique and highly organized extracellular matrix ultrastructures, which contribute to its high load-bearing capacity and light transmittance. Corneal collagen fibrils are controlled during development by small leucine-rich proteoglycans (SLRPs) that regulate the fibril diameter and spacing in order to achieve the unique optical transparency. Cyclodextrins (CDs) of varying size and chemical functionality for their ability to regulate collagen assembly during vitrification process are screened in order to create biosynthetic materials that mimic the native cornea structure. Addition of βCD to collagen vitrigels produces materials with aligned fibers and lamellae similar to native cornea, resulting in mechanically robust and transparent materials. Biochemistry analysis revealed that CD interacts with hydrophobic amino acids in collagen to influence assembly and fibril organization. To translate the self-assembled collagen materials for cornea reconstruction, custom molds for gelation and vitrification are engineered to create βCD/Col implants with curvature matching that of the cornea. Acellular βCD/Col materials are implanted in a rabbit partial keratoplasty model with interrupted sutures. The implants demonstrate tissue integration and support re-epithelialization. Therefore, the addition of CD molecules regulates collagen self-assembly and provides a simple process to engineer corneal mimetic substitutes with advanced structural and functional properties.

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“…SHG microscopy can be also configured to extract more information on the collagen architecture and preferred organization throughout stromal depth through polarisation resolved methods 21 . It should be object of further investigation if this methodology could be used to detect the biosynthesis (fibrillogenesis) or remodelling of collagen in bio-engineered scaffolds 1, 23, 28, 29 .…”

Section: Discussionmentioning

confidence: 99%

Multimodal imaging quality control of epithelia regenerated with cultured human donor corneal limbal epithelial stem cells

Lombardo

Serrao

Barbaro

et al. 2017

Sci Rep

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Current imaging techniques for the characterization of differentiated corneal limbal stem cells are destructive and cannot be used in eye bank for monitoring the regenerated epithelium in culture. We presented a minimally invasive, multimodal, marker-free imaging method for the investigation of epithelia regenerated with cultured human donor corneal limbal epithelial stem cells. Two-photon fluorescence and harmonic generation signals were collected from specimens in culture and used for evaluating the structure and morphology of epithelia cultured on two different bio-scaffolds; in addition, donor human corneal tissues were used as controls. The method provided reliable information on the organization of cellular and extracellular components of biomaterial substrates and was highly sensitive to determine differences between the density packing arrangement of epithelial cells of different biomaterials without relying on inferences from exogenous labels. The present minimally invasive standardized quality control methodology can be reliably translated to eye banks and used for monitoring harvested corneal limbal stem cells growth and differentiation in bioengineered materials.

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Development of human corneal epithelium on organized fibrillated transparent collagen matrices synthesized at high concentration

Cited by 31 publications

References 38 publications

Topotactic Fibrillogenesis of Freeze-Cast Microridged Collagen Scaffolds for 3D Cell Culture

Topotactic Fibrillogenesis of Freeze-Cast Microridged Collagen Scaffolds for 3D Cell Culture

Cyclodextrin Modulated Type I Collagen Self‐Assembly to Engineer Biomimetic Cornea Implants

Multimodal imaging quality control of epithelia regenerated with cultured human donor corneal limbal epithelial stem cells

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