Development of highly sensitive electrochemical genosensor based on multiwalled carbon nanotubes–chitosan–bismuth and lead sulfide nanoparticles for the detection of pathogenic Aeromonas

Fernandes, António Maximiano; Abdalhai, Mandour H.; Ji, Jian; Xi, Bingwen; Xie, Jun; Sun, Jiadi; Rasoamandrary, Noeline; Lee, Byong H.; Sun, Xiulan

doi:10.1016/j.bios.2014.07.054

Cited by 57 publications

(22 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…3B which was attributed to more negative charges at the electrode surface that would make the interfacial electron transfer even more difficult [33]. The results were consistent with those obtained from CV measurements.…”

Section: Electrochemical Characterization Of Biosensorsupporting

confidence: 85%

“…In all of them labeled ssDNA were used which has a difficult and expensive biochemical preparation [27,32,33].In this study, we used a new methodology for immobilization of ssDNA sequence on glassy carbon electrode. Reduced graphene oxide decorated cerium oxide nanocomposite/Polyaniline/glassy carbon electrode [CeO 2 NPs-RGO/PANI/GCE] was fabricated as a platform for immobilization of ssDNA sequence.…”

Section: A N U S C R I P Tmentioning

confidence: 99%

See 1 more Smart Citation

Detection of Aeromonas hydrophila DNA oligonucleotide sequence using a biosensor design based on Ceria nanoparticles decorated reduced graphene oxide and Fast Fourier transform square wave voltammetry

Jafari

Faridbod

Norouzi

et al. 2015

Analytica Chimica Acta

View full text Add to dashboard Cite

Section: Electrochemical Characterization Of Biosensorsupporting

confidence: 85%

Section: A N U S C R I P Tmentioning

confidence: 99%

Detection of Aeromonas hydrophila DNA oligonucleotide sequence using a biosensor design based on Ceria nanoparticles decorated reduced graphene oxide and Fast Fourier transform square wave voltammetry

Jafari

Faridbod

Norouzi

et al. 2015

Analytica Chimica Acta

View full text Add to dashboard Cite

“…Electrochemical biosensors are some of the most promising platforms with the potential to achieve these goals and have been used to detect a number of analytes. These include, e.g., (1) disease biomarkers and pathogenic proteins in serum [ 1 , 2 ], human plasma [ 3 , 4 ] or urine [ 5 ]; (2) Nucleic acids such as DNA samples from polymerase chain reaction (PCR) products [ 6 , 7 ] and microRNAs [ 2 , 8 ]; (3) microorganisms in seawater medium [ 9 ] and tap water [ 10 ]; (4) toxins in food samples [ 11 ]; and (5) drugs levels in human serum [ 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Optimisation and Characterisation of Anti-Fouling Ternary SAM Layers for Impedance-Based Aptasensors

Miodek

Regan

Bhalla

et al. 2015

Sensors

View full text Add to dashboard Cite

An aptasensor with enhanced anti-fouling properties has been developed. As a case study, the aptasensor was designed with specificity for human thrombin. The sensing platform was developed on screen printed electrodes and is composed of a self-assembled monolayer made from a ternary mixture of 15-base thiolated DNA aptamers specific for human thrombin co-immobilised with 1,6-hexanedithiol (HDT) and further passivated with 1-mercapto-6-hexanol (MCH). HDT binds to the surface by two of its thiol groups forming alkyl chain bridges and this architecture protects from non-specific attachment of molecules to the electrode surface. Using Electrochemical Impedance Spectroscopy (EIS), the aptasensor is able to detect human thrombin as variations in charge transfer resistance (Rct) upon protein binding. After exposure to a high concentration of non-specific Bovine Serum Albumin (BSA) solution, no changes in the Rct value were observed, highlighting the bio-fouling resistance of the surface generated. In this paper, we present the optimisation and characterisation of the aptasensor based on the ternary self-assembled monolayer (SAM) layer. We show that anti-fouling properties depend on the type of gold surface used for biosensor construction, which was also confirmed by contact angle measurements. We further studied the ratio between aptamers and HDT, which can determine the specificity and selectivity of the sensing layer. We also report the influence of buffer pH and temperature used for incubation of electrodes with proteins on detection and anti-fouling properties. Finally, the stability of the aptasensor was studied by storage of modified electrodes for up to 28 days in different buffers and atmospheric conditions. Aptasensors based on ternary SAM layers are highly promising for clinical applications for detection of a range of proteins in real biological samples.

show abstract

“…Ligaj et al presented two kinds of electrochemical DNA sensor for the detection of pathogenic bacteria [30]. Fernandes et al reported the construction of electrochemical genosensor based on multiwalled carbon nanotube-chitosan-bismuth and lead sulfide nanoparticle for the detection of pathogenic Aeromonas [31]. Our group applied an electrochemical DNA sensor based on carboxyl-functionalized graphene oxide and poly-L-lysine-modified electrode for the detection of tlh gene sequences that related to Vibrio parahaemolyticus [32].…”

Section: Introductionmentioning

confidence: 98%

Electrochemical DNA sensor for Staphylococcus aureus nuc gene sequence with zirconia and graphene modified electrode

Sun

Wang

et al. 2015

J Solid State Electrochem

View full text Add to dashboard Cite

In this paper, zirconia (ZrO 2 ) and graphene (GR) nanocomposite was electrodeposited on the surface of carbon ionic liquid electrode (CILE), which was used to construct an electrochemical DNA biosensor. GR was electroreduced from graphene oxide by potentiostatic method, and ZrO 2 nanoparticle was further electrodeposited on GR/CILE by cycling voltammetric scan in a ZrOCl 2 solution. The presence of GR on the electrode surface can provide a highly conductive interface with large surface area for the loading of ZrO 2 nanoparticles. Single-stranded DNA (ssDNA) probe sequences with phosphate group at the 5′ end could be easily immobilized on the surface of ZrO 2 /GR/CILE due to the strong affinity between ZrO 2 and phosphate groups. The ssDNA/ZrO 2 /GR/CILE was applied to hybridize with the target ssDNA sequence, and methylene blue (MB) was used as the electrochemical indicator. Due to the different binding models of MB with double-stranded DNA and ssDNA on the electrode surface, electrochemical response of MB was decreased after the hybridization reaction. Under the optimal conditions, the reduction peak current of MB was proportional to the concentration of Staphylococcus aureus nuc gene sequence in the range from 1.0×10 −13 to 1.0×10 −6 mol L −1 with the detection limit of 3.23 × 10 −14 mol L −1 (3σ). The electrochemical DNA sensor exhibited good selectivity to various mismatched ssDNA sequences, and the polymerase chain reaction amplification products of S. aureus nuc gene sequence were further detected with satisfactory results. Therefore, this electrochemical DNA sensor with ZrO 2 nanoparticles and GR nanosheet modified electrode could be used for the detection of specific ssDNA sequence in real biological samples.

show abstract

Development of highly sensitive electrochemical genosensor based on multiwalled carbon nanotubes–chitosan–bismuth and lead sulfide nanoparticles for the detection of pathogenic Aeromonas

Cited by 57 publications

References 53 publications

Detection of Aeromonas hydrophila DNA oligonucleotide sequence using a biosensor design based on Ceria nanoparticles decorated reduced graphene oxide and Fast Fourier transform square wave voltammetry

Detection of Aeromonas hydrophila DNA oligonucleotide sequence using a biosensor design based on Ceria nanoparticles decorated reduced graphene oxide and Fast Fourier transform square wave voltammetry

Optimisation and Characterisation of Anti-Fouling Ternary SAM Layers for Impedance-Based Aptasensors

Electrochemical DNA sensor for Staphylococcus aureus nuc gene sequence with zirconia and graphene modified electrode

Contact Info

Product

Resources

About