Development of highly polymorphic genomic microsatellite markers and their application to gene flow in a natural population of <i>Abies firma</i>

Iwaizumi, Masakazu G.; Ohtani, Mitsuaki; Nasu, Jin'ya; Takahashi, Makoto

doi:10.1080/13416979.2019.1663579

Cited by 6 publications

(3 citation statements)

References 23 publications

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“…According to the results of the present study, the genetic diversity (H e ) of A. nephrolepis was found to be 0.791 on average, which was higher than that of A. koreana (H e = 0.759) [47]. Compared to other Abies species, such as A. firma (0.747) [48], A. alba (0.724) [49], A. cilicica (0.689) [50], A. pinsapo (0.596) [51], A. fraseri (0.442) [52], and A. ziyuanensis (0.337) [6], A. nephrolepis showed relatively high genetic diversity. Additionally, the genetic diversity of coniferous species analyzed using nSSR markers was reported to be 0.815 for Picea abies [53], 0.79 for Pinus sylvestris [54], 0.704 for Nothotsuga longibracteata [55], 0.652 for Picea omorika [56], 0.610 for Pinus koraiensis [57], and 0.406 for Picea jezoensis [58].…”

Section: Genetic Diversity Of a Nephrolepis Populationscontrasting

confidence: 42%

Selection of Abies nephrolepis Materials for Restoration of Genetic Diversity in Mt. Gariwangsan Degraded Area

2023

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This study proposes a strategy for selecting materials for Abies nephrolepis, a key subalpine species, to restore damaged forests in Mt. Gariwangsan while considering genetic diversity. The genetic diversity distribution of A. nephrolepis in Korea was evaluated using nuclear simple-sequence repeat (nSSR) markers and the genetic differences between populations were analyzed. A suitable genetic restoration population was selected and a strategy for selecting restoration materials was presented. The genetic diversity indices of 326 individuals across 10 populations, and the genetic relationships between populations, were analyzed using AMOVA, principal coordinate analysis, UPGMA, and STRUCTURE analysis. The genetic diversity of A. nephrolepis populations averaged 0.791, indicating relatively high genetic diversity. The results showed that the genetic differentiation pattern of A. nephrolepis was divided into two groups: Mt. Jungwangsan and other populations. Therefore, genetically diverse restoration materials can be secured from the Mt. Gariwangsan population to restore damaged areas. We found that individuals distributed within approximately 10 m were autocorrelated due to their high genetic similarity, whereas they were randomly distributed in the range of 15 m or more. The optimal sampling strategy involved collecting seeds from a minimum of 25 individuals within 10 m. This study suggests that genetic diversity in restoration material selection is necessary to enhance adaptability to environmental changes.

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Section: Genetic Diversity Of a Nephrolepis Populationscontrasting

confidence: 42%

Selection of Abies nephrolepis Materials for Restoration of Genetic Diversity in Mt. Gariwangsan Degraded Area

2023

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“…1, Uchiyama et al (); 2, Hansen et al (); 3, Saito et al (); 4, Lian et al (); 5, Iwaizumi et al ().…”

Section: Methodsmentioning

confidence: 99%

“…We used 13 nuclear microsatellite (SSR) loci, which were previously developed for several Abies species (Hansen, Vendramin, Sebastiani, & Edwards, 2005;Saito, Lian, Hogetsu, & Ide, 2005;Lian, Goto, & Hogetsu, 2007;Uchiyama, Fujii, Ishizuka, Goto, & Tsumura, 2013;Iwaizumi, Ohtani, Nasu, & Takahashi, 2019; Table 1). Multiplex PCRs were performed using methods described elsewhere (Aizawa et al, 2018).…”

Section: Nuclear Microsatellitesmentioning

confidence: 99%

Natural hybridization and introgression of Abies firma and Abies homolepis along the altitudinal gradient and genetic insights into the origin of Abies umbellata

Aizawa

Iwaizumi

2020

Plant Species Biology

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Two Japanese fir species (Pinaceae), Abies firma and A. homolepis, comprise the majority of the temperate forest vegetation zones of Japan. Their vertical distributions are exclusively segregated: A. firma occurs in low elevations and A. homolepis occurs in elevated regions; they contact each other at an altitude of around 1,000–1,100 m. Around the contact zone, A. umbellata is described as a tree with shoots of A. homolepis. It has the greenish cones of A. firma instead of the violet A. homolepis cones. An albinism hypothesis and a hybrid hypothesis were proposed to explain the origin of A. umbellata, provoking long debates. Therefore, we examined a total of 78 A. firma and A. homolepis trees (including an A. umbellata tree) along the altitudinal gradient in two study sites using 13 nuclear microsatellites and a species‐specific chloroplast DNA (cpDNA) marker and examined morphologies of shoots and cone scales. Finally, we tested the two hypotheses. The genetic analyses revealed that shoot morphologies of A. homolepis and A. firma correspond mostly to the genetic identity of the species, whereas nine trees with intermediate shoot morphologies and two trees (including the A. umbellata tree) with A. homolepis shoots were genetically intermediate, indicating natural hybrids of A. firma and A. homolepis. These 11 hybrids occur in the narrow altitude ranges of their contact zones. The genetic analyses also suggested that hybrid backcrosses with parental species occur. These results support the hybrid hypothesis and suggest that the natural hybrids should taxonomically be classified as A. × umbellata.

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Differences in climate and drought response of the exotic plantation species Abies firma, Cryptomeria japonica, and Chamaecyparis obtusa in southern Korea

Jung

Kim

Jung

et al. 2020

Journal of Forest Research

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Development of highly polymorphic genomic microsatellite markers and their application to gene flow in a natural population of Abies firma

Cited by 6 publications

References 23 publications

Selection of Abies nephrolepis Materials for Restoration of Genetic Diversity in Mt. Gariwangsan Degraded Area

Selection of Abies nephrolepis Materials for Restoration of Genetic Diversity in Mt. Gariwangsan Degraded Area

Natural hybridization and introgression of Abies firma and Abies homolepis along the altitudinal gradient and genetic insights into the origin of Abies umbellata

Differences in climate and drought response of the exotic plantation species Abies firma, Cryptomeria japonica, and Chamaecyparis obtusa in southern Korea

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