Development of highly glyphosate-tolerant tobacco by coexpression of glyphosate acetyltransferase gat and EPSPS G2-aroA genes

Dun, Baoqing; Wang, Xujing; Lu, Wei; Chen, Ming; Zhang, Wei; Ping, Shuzhen; Wang, Zhixing; Zhang, Baoming; Lin, Min

doi:10.1016/j.cj.2014.03.003

Cited by 27 publications

(17 citation statements)

References 12 publications

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“…G2-EPSPS was identified from Pseudomonas fluorescens G2 isolated from a storage area with a history of glyphosate pollution and showed high glyphosate tolerance in transgenic tobacco and maize ( Zhu et al, 2003 ; Dun et al, 2007 ; Liu et al, 2015 ). Dun et al (2014) reported that the transgenic tobacco contained G2-EPSPS/GAT showed higher tolerance to glyphosate than tobacco contained only G2-EPSPS or GAT alone, and G2-EPSPS carrying tobaccos were significantly more susceptible to glyphosate than tobacco contained GAT only. The combination of the metabolic detoxification and target enzyme of glyphosate may result in high tolerance in transgenic crops, providing a new strategy for developing glyphosate tolerant crops.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, overexpression of the GOX or GAT gene can also result in a relatively high level of glyphosate tolerance in different crops ( Green et al, 2008 ; Hadi et al, 2012 ). Dun et al (2014) compared the glyphosate tolerance of transgenic tobacco which expressed GAT and EPSPS alone or combination and the results suggested that co-expression of them showed the highest tolerance to glyphosate, providing a new method to develop high glyphosate tolerant crops by combination of different strategies.…”

Section: Introductionmentioning

confidence: 99%

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Co-expression of G2-EPSPS and glyphosate acetyltransferase GAT genes conferring high tolerance to glyphosate in soybean

Guo

Hong

et al. 2015

Front. Plant Sci.

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Glyphosate is a widely used non-selective herbicide with broad spectrum of weed control around the world. At present, most of the commercial glyphosate tolerant soybeans utilize glyphosate tolerant gene CP4-EPSPS or glyphosate acetyltransferase gene GAT separately. In this study, both glyphosate tolerant gene G2-EPSPS and glyphosate degraded gene GAT were co-transferred into soybean and transgenic plants showed high tolerance to glyphosate. Molecular analysis including PCR, Sothern blot, qRT-PCR, and Western blot revealed that target genes have been integrated into genome and expressed effectively at both mRNA and protein levels. Furthermore, the glyphosate tolerance analysis showed that no typical symptom was observed when compared with a glyphosate tolerant line HJ06-698 derived from GR1 transgenic soybean even at fourfold labeled rate of Roundup. Chlorophyll and shikimic acid content analysis of transgenic plant also revealed that these two indexes were not significantly altered after glyphosate application. These results indicated that co-expression of G2-EPSPS and GAT conferred high tolerance to the herbicide glyphosate in soybean. Therefore, combination of tolerant and degraded genes provides a new strategy for developing glyphosate tolerant transgenic crops.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Co-expression of G2-EPSPS and glyphosate acetyltransferase GAT genes conferring high tolerance to glyphosate in soybean

Guo

Hong

et al. 2015

Front. Plant Sci.

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“…The lines with lower gene expression and HR showed characteristic chlorosis in all plant tissues and some of these lines recovered from glyphosate stress within few weeks. Lower EPSPS gene expression has been previously reported by Dun et al (2014) in transgenic crops including soybean, maize, potato, mustard, sugar beet, and tomato which displayed such chlorotic symptoms. The enzyme activity exhibited direct correlation with the amount of mRNA detected in the qRT-PCR.…”

Section: Discussionmentioning

confidence: 99%

Genetically transformed tobacco plants expressing synthetic EPSPS gene confer tolerance against glyphosate herbicide

Imran

Asad

Barboza

et al. 2017

Physiol Mol Biol Plants

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Glyphosate quashes the synthesis of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme which intercedes the functioning of shikimate pathway for the production of aromatic amino acids. Herbicide resistant crops are developed using glyphosate insensitive EPSPS gene isolated from Agrobacterium sp. strain CP4, which give farmers a sustainable weed control option. Intentions behind this study were to design and characterize the synthetic herbicide resistant CP4-EPSPS gene in a model plant system and check the effectiveness of transformed tobacco against application of glyphosate. Putative transgenic plants were obtained from independent transformation events, and stable plant transformation, transgene expression and integration were demonstrated respectively by PCR, qRT-PCR and Southern hybridization. Gene transcript level and gene copy number (1-4) varied among the tested transgenic tobacco lines. Herbicide assays showed that transgenic plants were resistant to glyphosate after 12 days of spraying with glyphosate, and EPSPS activity remained at sufficient level to withstand the spray at 1000 ppm of the chemical. T 1 plants analyzed through immunoblot strips and PCR showed that the gene was being translated into protein and transmitted to the next generation successfully. This codon optimized synthetic CP4-EPSPS gene is functionally equivalent to the gene for glyphosate resistance available in the commercial crops and hence we recommend this gene for transformation into commercial crops.

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“…GM crops resistant to the broad-spectrum herbicides glyphosate and glufosinate have first been cultivated commercially in the 1990s [5], and GM crops with resistance to other herbicides are under development [6], or already on the market, with various HR traits increasingly combined in one crop [7]. Another, more recent strategy is the development of plants that are resistant to high concentrations of glyphosate without exhibiting a yield drag [8, 9]. …”

Section: Reviewmentioning

confidence: 99%

Herbicide resistance and biodiversity: agronomic and environmental aspects of genetically modified herbicide-resistant plants

et al. 2017

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Farmland biodiversity is an important characteristic when assessing sustainability of agricultural practices and is of major international concern. Scientific data indicate that agricultural intensification and pesticide use are among the main drivers of biodiversity loss. The analysed data and experiences do not support statements that herbicide-resistant crops provide consistently better yields than conventional crops or reduce herbicide amounts. They rather show that the adoption of herbicide-resistant crops impacts agronomy, agricultural practice, and weed management and contributes to biodiversity loss in several ways: (i) many studies show that glyphosate-based herbicides, which were commonly regarded as less harmful, are toxic to a range of aquatic organisms and adversely affect the soil and intestinal microflora and plant disease resistance; the increased use of 2,4-D or dicamba, linked to new herbicide-resistant crops, causes special concerns. (ii) The adoption of herbicide-resistant crops has reduced crop rotation and favoured weed management that is solely based on the use of herbicides. (iii) Continuous herbicide resistance cropping and the intensive use of glyphosate over the last 20 years have led to the appearance of at least 34 glyphosate-resistant weed species worldwide. Although recommended for many years, farmers did not counter resistance development in weeds by integrated weed management, but continued to rely on herbicides as sole measure. Despite occurrence of widespread resistance in weeds to other herbicides, industry rather develops transgenic crops with additional herbicide resistance genes. (iv) Agricultural management based on broad-spectrum herbicides as in herbicide-resistant crops further decreases diversity and abundance of wild plants and impacts arthropod fauna and other farmland animals. Taken together, adverse impacts of herbicide-resistant crops on biodiversity, when widely adopted, should be expected and are indeed very hard to avoid. For that reason, and in order to comply with international agreements to protect and enhance biodiversity, agriculture needs to focus on practices that are more environmentally friendly, including an overall reduction in pesticide use. (Pesticides are used for agricultural as well non-agricultural purposes. Most commonly they are used as plant protection products and regarded as a synonym for it and so also in this text.)Electronic supplementary materialThe online version of this article (doi:10.1186/s12302-016-0100-y) contains supplementary material, which is available to authorized users.

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Development of highly glyphosate-tolerant tobacco by coexpression of glyphosate acetyltransferase gat and EPSPS G2-aroA genes

Cited by 27 publications

References 12 publications

Co-expression of G2-EPSPS and glyphosate acetyltransferase GAT genes conferring high tolerance to glyphosate in soybean

Co-expression of G2-EPSPS and glyphosate acetyltransferase GAT genes conferring high tolerance to glyphosate in soybean

Genetically transformed tobacco plants expressing synthetic EPSPS gene confer tolerance against glyphosate herbicide

Herbicide resistance and biodiversity: agronomic and environmental aspects of genetically modified herbicide-resistant plants

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