Development of high throughput microfluidic cell culture chip for perfusion 3-dimensional cell culture-based chemosensitivity assay

Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors

“…Therefore, 3D models can better replicate intrinsic physiological conditions and in vivo cellular responses to external stimuli compared to the 2D monolayer. 77,78,[80][81][82] As such, 3D-cultured cells can be the ideal sensing element in cell-based sensors to provide the most biologically relevant information and predictive data for in vivo tests. Cells grown in 3D culture can be incorporated into a biosensor either by direct attachment onto a biotic or abiotic substrate surface or by indirect attachment via entrapment in a biocompatible biopolymer.…”

Microbioreactors for Cartilage Tissue Engineering

“…1a -(III)), the cylindrical chamber, the smaller microchannel for medium fl ow, the microbioreactor chamber, and the waste medium reservoir are in layer B. Layer C consists of multiple through holes (D: 1.5 mm), allowing the insertion of their corresponding cylinders (D: 1.5 mm, H: 1.0 mm) on layer D. In this work, there is a shallow cylindrical cavity with specifi c dimensions (e.g., D: 1 mm, H: 250 μm) on the top surface of each cylinder (Fig. 1b ), which is not only used to accommodate a cell/scaffold for 3D cell culture but also to quantitatively defi ne the volume of such sample loading [ 9 ].…”

“…1 , and the associated devices: Fig. 2 ) as previously published [ 9 ] will be used as an example for description purposes. Fig.…”

“…1 The ( a ) design and ( b ) structure of a micro-scale perfusion cell culture chip based on microfl uidic technology (reprinted with permission from ref. [ 9 ]. Copyright 2011 Elsevier)…”

Automatic 3D Cell Analysis in High-Throughput Microarray Using Micropillar and Microwell Chips

“…[7][8][9][10] Thus, the ability to predict the clinical response of new compounds in 2D cell-based HTS is limited. 2,7,11 This lack of predictability may arise because such systems do not mimic the response of cells in the 3D microenvironment of in vivo tissues or tumors.…”