Development of high-performance thin layer chromatography method for identification of phenolic compounds and quantification of rosmarinic acid content in some species of the Lamiaceae family

Abstract

A BSTRACT Aim: Representatives of Nepetoideae Burnett subfamily are promising species of the Lamiaceae Martinov family because of accumulating such valuable groups of secondary metabolites as terpenoids and polyphenols. A high-performance thin layer chromatography (HPTLC) fingerprint method for the qualitative determination of phenolic compounds and for the quantification of rosmarinic acid (RA) content in methanol extracts of five species of this subfamily was developed for the first time. Materials and Methods: Dried aerial parts of Dracocephalum moldavica L., Ocimum americanum L., Lophanthus anisatus (Nutt.) Benth., Monarda fistulosa L., and Satureja hortensis L. collected in flowering period were macerated with methanol. The HPTLC analysis was conducted using the CAMAG analytical system (Muttenz, Switzerland). The comparative analysis of RA contents was performed by HPTLC densitometric detection at λ = 366nm. Results: Identification of polyphenols in the investigated herbs was performed by comparison of a color and R f of the chromatographic zones with six reference standards: rutin, apigenin, luteolin, caffeic acid, chlorogenic acid, and RA. HPTLC method was also validated for the quantification of RA in the extracts of investigated herbs. RA contents decreased in such a sequence: D. moldavica (24.83 ± 0.78mg/g) > M. fistulosa (20.32 ± 0.64mg/g) > O. americanum (19.59 ± 0.61mg/g) > S. hortensis (18.77 ± 0.52mg/g) > L. anisatus (12.61 ± 0.43mg/g). Conclusion: Obtained data can facilitate the differentiation of investigated species using the chromatographic fingerprints of their phenolic compounds. Developed and validated HPTLC method provides an approach to estimate RA content as a common marker of investigated herbs.

Polyphenols and Pharmacological Screening of a Monarda fistulosa L. dry Extract Based on a Hydrodistilled Residue By-Product

“…Furthermore, several unidentified fluorescent zones in different shades of blue and yellow colors were visible in the chromatograms of the test solution, especially after the derivatization. These spots could be considered as hydroxycinnamic acids or flavonoids, respectively ( Stanek et al, 2019 ; Shanaida et al, 2020 ). Similar results were found for the methanol extract of the M. fistulosa herb ( Shanaida et al, 2020 ).…”

“…The HPTLC analysis of phenolic compounds was carried out using a CAMAG analytical system (Switzerland) ( Stanek et al, 2019 ; Shanaida et al, 2020 ). The MFDE was dissolved in methanol (1.0 mg/mL) and filtered through a 0.45 μm Millipore filter to obtain a test solution.…”

“…The other groups of biologically active compounds of Monarda genus representatives such as flavonoids or hydroxycinnamic acids were studied less. Some of the scientific studies about the polyphenols composition of these species were published not only in the peer-reviewed journals ( Banach and Olechnowicz-Stepien, 1986 ; Davies and Mazza, 1992 ; Savickiene et al, 2002 ; Krasyuk and Pupyrkina, 2016 ; Shanaida et al, 2018a ; Shanaida et al, 2020 ; Vysochina, 2020 ). Flavonoids such as rutin, hyperoside, quercetin, quercitrin, and luteolin were found in the leaves and flowers of M. didyma grown in Lithuania by the HPLC method ( Savickiene et al, 2002 ).…”

“…( Krasyuk and Pupyrkina, 2016 ). Rosmarinic acid was found to be the main component of aqueous and methanol extracts obtained from the M. fistulosa herb grown in Ukraine ( Shanaida et al, 2018a ; Shanaida et al, 2020 ). The preventive and healing properties of polyphenols are mostly related to their antioxidant effects which could be useful in the treatment of inflammation, pain, cardiovascular diseases, neurodegenerative disorders, and cancer ( Pudziuvelyte et al, 2020 ).…”

Essential Oils from Monarda fistulosa: Chemical Composition and Activation of Transient Receptor Potential A1 (TRPA1) Channels

“…A number of phytochemical studies on Monarda species resulted in the identification of a wide spectrum of phytoconstituents, including phenolics [4,5], triterpenoids [6], monoterpene glycosides [7], carbohydrate, carotenoids, and vitamin C [8], with the phenolic complex containing flavonoids [5], anthocyanins, and phenolic acids. One compound specific to the Monarda genus is monardaein [9].…”