Development of high performance liquid chromatography method for miconazole analysis in powder sample

Aim: The present study aimed to describe the use of miconazole in the outpatient setting of a tertiary hospital in Riyadh Region. Methodology: This was a retrospective study that included reviewing the electronic prescriptions that contained miconazole among patients in a public hospital in Riyadh Region. Results: Most of the patients who received miconazole were females (89.29%) and the age of 51.79% of them was between 30 and 39 years. Most of the prescriptions were written by residents (96.43%) and most of the prescriptions were prescribed by emergency department (66.07%). Most of the patients received miconazole as vaginal suppository (50.00%) or as cream (39.29%). Conclusion: The present study showed that miconazole was uncommonly prescribed in in Riyadh Region. Further studies are needed to explore the frequency and pattern of miconazole use as well as to explore the frequency of prescribing other antifungal medications in different settings.

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“…Hermawan et al reported that miconazole is commonly used to the skin or to mucous membrane to cure fungal infections [15].…”

Section: Resultsmentioning

confidence: 99%

The Outpatient Prescribing of Miconazole in a Tertiary Hospital in Riyadh Region

Ahmed

Yusufoğlu

2021

JPRI

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“…The proposed method is much easier than HPLC methods stipulated in Pharmacopeias [ 1 , 15 ] and other existing analytical methods [ 3 – 11 ]. The reagents employed in the developed technique are cheaper and readily available, and the procedures do not involve any critical reaction conditions or tedious sample preparation.…”

Section: Resultsmentioning

confidence: 99%

“…Several analytical methods have been reported for the determination of miconazole in biological samples, pure and pharmaceutical dosage forms using various chromatographic methods such as high-performance liquid chromatography (HPLC) [ 3 – 7 ], gas chromatography (GC) [ 6 , 8 , 9 ], high-performance thin-layer chromatography (HPTLC) [ 10 ], hyphenated technique (gas chromatography-mass spectroscopy, GC-MS) [ 11 ], and spectrophotometry [ 12 – 14 ]. The official pharmacopoeial methods such as the United States Pharmacopeia (USP) and British Pharmacopoeia (BP) have recommended HPLC for miconazole nitrate assay in pharmaceutical preparations [ 1 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of an Extractive Spectrophotometric Method for Miconazole Nitrate Assay in Pharmaceutical Formulations

Eticha

Kahsay

Hailu

et al. 2018

Journal of Analytical Methods in Chemistry

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A simple extractive spectrophotometric technique has been developed and validated for the determination of miconazole nitrate in pure and pharmaceutical formulations. The method is based on the formation of a chloroform-soluble ion-pair complex between the drug and bromocresol green (BCG) dye in an acidic medium. The complex showed absorption maxima at 422 nm, and the system obeys Beer's law in the concentration range of 1–30 µg/mL with molar absorptivity of 2.285 × 104 L/mol/cm. The composition of the complex was studied by Job's method of continuous variation, and the results revealed that the mole ratio of drug : BCG is 1 : 1. Full factorial design was used to optimize the effect of variable factors, and the method was validated based on the ICH guidelines. The method was applied for the determination of miconazole nitrate in real samples.

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“…7 ml phosphate buffer was used as a diffusion medium and maintained at a pH of 6.8, containing 0.1% polysorbate 80 to overcome sink limitation and a temperature of 32 ± 2 °C. The magnetic stirrer was fixed at 400–420 rpm, and at the fixed interval of time, samples were removed and analyzed by high-performance liquid chromatography (HPLC) (Hermawan et al., 2017 ). For this purpose, C 8 column was used, and the mobile phase contained a mixture of methanol and water (85:15).…”

Section: Methodsmentioning

confidence: 99%

Development, optimization, and evaluation of a nanostructured lipid carrier of sesame oil loaded with miconazole for the treatment of oral candidiasis

et al. 2022

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Candida albicans is the fungus responsible for oral candidiasis, a prevalent disease. The development of antifungal-based delivery systems has always been a major challenge for researchers. This study was designed to develop a nanostructured lipid carrier (NLC) of sesame oil (SO) loaded with miconazole (MZ) that could overcome the solubility problems of MZ and enhance its antifungal activity against oral candidiasis. In the formulation of this study, SO was used as a component of a liquid lipid that showed an improved antifungal effect of MZ. An optimized MZ-loaded NLC of SO (MZ-SO NLC) was used, based on a central composite design-based experimental design; the particle size, dissolution efficiency, and inhibition zone against oral candidiasis were chosen as dependent variables. A software analysis provided an optimized MZ-SO NLC with a particle size of 92 nm, dissolution efficiency of 88%, and inhibition zone of 29 mm. Concurrently, the ex vivo permeation rate of the sheep buccal mucosa was shown to be significantly ( p < .05) higher for MZ-SO NLC (1472 µg/cm 2 ) as compared with a marketed MZ formulation (1215 µg/cm 2 ) and an aqueous MZ suspension (470 µg/cm 2 ). Additionally, an in vivo efficacy study in terms of the ulcer index against C. albicans found a superior result for the optimized MZ-SO NLC (0.5 ± 0.50) in a treated group of animals. Hence, it can be concluded that MZ, through an optimized NLC of SO, can treat candidiasis effectively by inhibiting the growth of C. albicans .

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Development of high performance liquid chromatography method for miconazole analysis in powder sample

Cited by 9 publications

References 11 publications

The Outpatient Prescribing of Miconazole in a Tertiary Hospital in Riyadh Region

The Outpatient Prescribing of Miconazole in a Tertiary Hospital in Riyadh Region

Development and Validation of an Extractive Spectrophotometric Method for Miconazole Nitrate Assay in Pharmaceutical Formulations

Development, optimization, and evaluation of a nanostructured lipid carrier of sesame oil loaded with miconazole for the treatment of oral candidiasis

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