Development of High-Performance and Rapid Immunoassay for Model Food Allergen Lysozyme Using Antibody-Conjugated Bacterial Magnetic Particles and Fully Automated System

Abstract

A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contains a reaction station, tip rack, and an eight-tip pipettor that is able to attach and detach a strong magnet to the tip surface. The immunoreaction time was shortened to 5 min, and the assay was completed within 20 min. The lower detection limit for lysozyme was 10 ng/mL. This system can be used to perform 24 samples in 60 min within 10% coefficient of variation.

Segmentation of hip cartilage in compositional magnetic resonance imaging: A fast, accurate, reproducible, and clinically viable semi‐automated methodology

“…Moreover, Siversson et al required the use of 20 Intel Xeon cores to achieve their fully automated approach. It is also worth noting that other authors who have published automated and semi‐automated methodology for segmentation of the hip such as Nishii et al, Li et al, Sato et al, and Xia et al have made no mention of the processing time or processing power required for their methodology. While each of these segmentations was carried out on a different dataset and using different computing power, it nevertheless makes sense to compare the order of magnitude of the time taken and the (extensive or otherwise) prerequisites required by each method.…”

Association of salivary lysozyme and C‐reactive protein with metabolic syndrome

“…SLZ was quantified at the Kuopio University research laboratory by the modified lysoplate method utilizing Micrococcus lysodeikticus (Sigma Chemical Co., St. Louis, MO, USA) and human milk lysozyme (Sigma Chemical Co.) and bovine serum albumin (Sigma Chemical Co.) as standards according to the methods used previously (Rudney & Smith 1985). The reported coefficient of variation (CV) of lysozyme was between 4.2% and10% (Sato et al 2001, Desai et al 2006).…”

Current research activity in biosensors

“…The assay using the complexes was fully automated [129]. Other applications of modified BMPs include discrimination of tuna [130], magnetic separation of DNA [131], immunoassay for a food allergen [132], capillary immunoassay of human chorionic gonadotrophin [133], and detection of HbA1c [134]. Modified BMP technology will be expanded to the development of biochips and other biosensing devices.…”