Development of high penicillin producing strains for solid state fermentation

Solid-State Fermentation of Agricultural Residues for the Production of Antibiotics

“…the sugarcane bagasse of particle size 14 mm increased the penicillin production by 37 %. However, this effect was due to higher sugar concentration in bagasse fraction (Barrios-Gonzalez et al 1993 ).…”

“…Clones of four industrial strains of P. chrysogenum producing higher penicillin were subjected to assess its capacity to produce high quantity of antibiotic in SSF (Barrios-Gonzalez et al 1993 ). S. rimosus TM-55 is treated with 3 % EMS, and 29 auxotrophic mutants (AM-1 to AM-29) are isolated from 5,457 colonies.…”

Improvement of penicillin yields in solid-state and submerged fermentation of Penicillium chrysogenum by amplification of the penicillin biosynthetic gene cluster

“…P. chrysogenum P2-32 is a high-producing strain; a reisolate of P2 showing good performance in SSF, while keeping its production level in submerged fermentation (SmF) (Barrios-González et al 1993a) and contains the penicillin gene cluster amplified several times in tandem repeats. P. chrysogenum NRRL 1951 (wild type) was used as source of DNA for the genomic library.…”

“…The cultures were made essentially as previously described (Barrios-González et al 1993a). Sugar-cane bagasse, grounded and sieved through a 0.3-0.6 mm mesh, was used as inert support.…”

“…Spores from each strain (1 9 10 6 ) were used to inoculate 50 ml of complex growth medium (Somerson et al 1961) in 250 ml flasks, these were incubated at 25°C, 250 rpm, for 36 h. Five milliliters of these cultures were then inoculated into 250 ml flasks containing 45 ml of complex production medium (Somerson et al 1961), in duplicate for each time point, they were incubated for 144 h at 25°C and 250 rpm (Barrios-González et al 1993a). Antibiotic production was quantified by bioassay against B. subtilis.…”