Development of High Frequency Microfluidic Biosensors for Intracellular Analysis

Dalmay, Claire; Leroy, Jonathan; Pothier, A.; Blondy, Pierre

doi:10.1016/j.proeng.2014.11.264

Cited by 10 publications

(6 citation statements)

References 8 publications

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“…Split ring resonators (SRRs) at 2-3 GHz have been used to detect [31] and even measure the dielectric properties of single, flowing polystyrene spheres [18]. Moreover, at frequencies above 2 GHz, single stationary or flowing polystyrene microspheres can be detected [18], [32]. Coupled dielectric-planar SRR resonator devices have been shown to increase the Q factor of the SRR sensing element by ~40 fold [33].…”

Section: Take-home Messagesmentioning

confidence: 99%

Microwave Dielectric Sensing of Free-Flowing, Single, Living Cells in Aqueous Suspension

Watts

Hanham

Armstrong

et al. 2020

IEEE J. Electromagn. RF Microw. Med. Biol.

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Where a licence is displayed above, please note the terms and conditions of the licence govern your use of this document.When citing, please reference the published version. Take down policyWhile the University of Birmingham exercises care and attention in making items available there are rare occasions when an item has been uploaded in error or has been deemed to be commercially or otherwise sensitive.

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Section: Take-home Messagesmentioning

confidence: 99%

Microwave Dielectric Sensing of Free-Flowing, Single, Living Cells in Aqueous Suspension

Watts

Hanham

Armstrong

et al. 2020

IEEE J. Electromagn. RF Microw. Med. Biol.

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“…Nevertheless, the efficacy of the technique of electrical impedance spectroscopy is limited at the cell membrane level. Whereas by applying high frequency electromagnetic waves (beyond hundreds of MHz), the waves can overcome the cell membrane and interact directly with the intracellular compartments [15], [16]. In addition, the demonstration of microwave sensing at the single cell level has already been performed [17]- [21].…”

Section: Introductionmentioning

confidence: 99%

Microwave Monitoring of Single Cell Monocytes Subjected to Electroporation

Tamra

Dubuc

Rols

et al. 2017

IEEE Trans. Microwave Theory Techn.

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This paper presents the monitoring of single cells subjected to electroporation using microwave dielectric spectroscopy. The experimental results showed first a clear distinction between two cell states: viable cells and affected ones by a chemical treatment (Saponin). It also pointed out a high correlation (R 2 > 0.94) with biological standard techniques in detecting the two types of electroporation: the reversible and irreversible ones. The developed microfluidic and microwavebased sensor exposes a decrease in the capacitive and conductive contrasts of the investigated single cells treated by irreversible electroporation indicating damages at the cellular level, while cells under reversible electroporation present a similar dielectric response to that of the nontreated cells. This result corresponds to results frequently employed in biological studies. More interestingly, a study of the kinetics of the cell's damage induction over time, by electroporation, has been experimentally done, which makes microwave dielectric spectroscopy an attractive technique for cell's electroporation researches.

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“…When using sub-GHz Electrical Impedance Spectroscopy (EIS), the membrane state (permeabilization) is indeed revealed [7]. Operating at microwaves (beyond few GHz) becomes then particularly interesting, because (1) the cell's membrane becomes transparent [8], and consequently (2) the electromagnetic fields penetrate deeply into the cell and interact directly with the intracellular content.…”

Section: Introductionmentioning

confidence: 99%

Microwave dielectric spectroscopy for single cell irreversible electroporation monitoring

Tamra

Deburghgraeve

Dubuc

et al. 2016

2016 IEEE MTT-S International Microwave Symposium (IMS)

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This paper demonstrates the efficient use of microwave dielectric spectroscopy for single cell irreversible electroporation monitoring. The experimental results point out a high correlation (R 2 higher than 0.94) with biological gold standard technique based on flow cytometry, whereas microwave approach features several key advantages: marker-less, contactless and in-liquid cell(s) monitoring. The developed microwave and microfluidic-based biosensor reveals an increase in the capacitance and the conductance of single cells under investigation subjected to irreversible electroporation reflecting an increase in cell damages, which is in great correlation with results routinely obtained in biology. More interestingly, the post electroporation damages of cell have been experimentally quantified over time, which makes the microwave dielectric spectroscopy a technique of interest for cell's electroporation researches.

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Development of High Frequency Microfluidic Biosensors for Intracellular Analysis

Cited by 10 publications

References 8 publications

Microwave Dielectric Sensing of Free-Flowing, Single, Living Cells in Aqueous Suspension

Microwave Dielectric Sensing of Free-Flowing, Single, Living Cells in Aqueous Suspension

Microwave Monitoring of Single Cell Monocytes Subjected to Electroporation

Microwave dielectric spectroscopy for single cell irreversible electroporation monitoring

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