Development of High Efficiency Micropropagation Protocol of an Adult Tree—Wrightia tomentosa

Purohit, S. D.; Joshi, P.; Tak, K.; Nagori, R.

doi:10.1007/1-4020-3213-7_15

Cited by 5 publications

(4 citation statements)

References 18 publications

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“…[21] improved multiplication in Prunus armeniaca L. by using 800 µM of phloroglucinol. [36] demonstratedthat 400 µM of phloroglucinol enhanced axillary shoot proliferation in Wrightia tomentosa Roem. et Schult.…”

Section: Discussionmentioning

confidence: 98%

Phloroglucinol is Effective for in vitro Growth and Multiplication of Musa accuminata Cv. Grand Naine Shoots and Roots

Londe

Vendrame

Oliveira

et al. 2017

JABB

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Despite being a major staple food in the world, banana production in the United States is still limited, with about 500 acres under cultivation. Micropropagation has been an effective method for the large-scale production of bananas to meet both domestic and international markets. However, the efficiency of micropropagation protocols depends on several factors, particularly on the types, combinations, and levels of plant growth regulators used in the culture media. Phloroglucinol is a growth regulator that acts synergistically with auxins and cytokinins. The use of phloroglucinol for Short Communication

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Section: Discussionmentioning

confidence: 98%

Phloroglucinol is Effective for in vitro Growth and Multiplication of Musa accuminata Cv. Grand Naine Shoots and Roots

Londe

Vendrame

Oliveira

et al. 2017

JABB

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“…The genetic variability was kept minimum by using a single field grown ortet, thus minimising statistical errors [ 16 ]. Nodal regions derived from the fresh flushes of growth from the ortet, two weeks after lopping one major branch served as the explants [ 17 ]. The nodal explants were conditioned over a period of 4 months (subculture/four weeks) on MS medium (1962) [ 18 ], pH 5.8 and 2 μM each of BAP and NAA for shoot multiplication.…”

Section: Methodsmentioning

confidence: 99%

Genetic programming based models in plant tissue culture: An addendum to traditional statistical approach

2018

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In this paper, we compared the efficacy of observation based modeling approach using a genetic algorithm with the regular statistical analysis as an alternative methodology in plant research. Preliminary experimental data on in vitro rooting was taken for this study with an aim to understand the effect of charcoal and naphthalene acetic acid (NAA) on successful rooting and also to optimize the two variables for maximum result. Observation-based modelling, as well as traditional approach, could identify NAA as a critical factor in rooting of the plantlets under the experimental conditions employed. Symbolic regression analysis using the software deployed here optimised the treatments studied and was successful in identifying the complex non-linear interaction among the variables, with minimalistic preliminary data. The presence of charcoal in the culture medium has a significant impact on root generation by reducing basal callus mass formation. Such an approach is advantageous for establishing in vitro culture protocols as these models will have significant potential for saving time and expenditure in plant tissue culture laboratories, and it further reduces the need for specialised background.

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“…Saha [14] also observed that aged explants leached out more phenolics in the medium. Purohit et al [15] had suggested trimming as a method of rejuvenation/ reinvigoration of mature adult woody plants. Vibha et al [16] obtained multiple shoots from nodal shoot segments of lopped tree of Dalbergia sissoo.…”

Section: Release Of Bud Growth Inhibitionmentioning

confidence: 99%

Problems Encountered during In vitro Culture Establishment in Terminalia arjuna

Choudhary

Arya

2020

ARRB

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The main aim of present study was to overcome the problems associated with the in vitro culture initiation in Terminalia arjuna. The micropropagation of tree species is not easy as shrubs and herbs. Many problems encountered from explant collection to in vitro culture establishment. The problems that have been occurred during T. arjuna micropropagation were culture contamination, phenolic exudation, bud growth inhibition, shoots yellowing and leaf fall. All these problems have been solved by applying certain treatments prior to explant collection and inoculation. The mother tree was lopped in November months (six months prior to explant collection) to remove any inhibitory substance and release bud growth. Different sterilizing agents were used to minimize the bacterial and fungal contamination. Some modification in culture media (use of different concentration of NH4NO3 and KNO3 salts and adenine sulphate) was done. Surface sterilization of nodal explants collected from lopped branches with 0.1% HgCl2 for 8 min., treatment with chilled antioxidant solution (Ascorbic acid, Citric acid and PVP) and half strength of NH4NO3 and KNO3 salts of MS medium supported 100% bud break response with proliferation of green and healthy in vitro shoots. Removing these hurdles already in the initial stage of micropropagation is very important and maximize mass in vitro propagation of this medicinally important Arjun tree.

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Development of High Efficiency Micropropagation Protocol of an Adult Tree—Wrightia tomentosa

Cited by 5 publications

References 18 publications

Phloroglucinol is Effective for in vitro Growth and Multiplication of Musa accuminata Cv. Grand Naine Shoots and Roots

Phloroglucinol is Effective for in vitro Growth and Multiplication of Musa accuminata Cv. Grand Naine Shoots and Roots

Genetic programming based models in plant tissue culture: An addendum to traditional statistical approach

Problems Encountered during In vitro Culture Establishment in Terminalia arjuna

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