Development of Gut-Mucus Chip for Intestinal Absorption Study

Advances in the Evaluation of Gastrointestinal Absorption Considering the Mucus Layer

“…Culture environments that are difficult to replicate with conventional cell culture systems, such as mechanical stimulation, 3D extracellular matrix environments, and cell–cell interactions, can be constructed using these microfluidic devices [ 93 , 94 , 95 , 96 ]. Caco-2 cells have been cultured on microfluidic devices and used to assess drug cell membrane permeability under conditions that mimic the intestinal environment [ 97 , 98 , 99 ]. Although MUC2 was not expressed in Caco-2 cells cultured in normal transwell inserts, it was expressed in Caco-2 cells cultured on microfluidic devices [ 100 , 101 ].…”

Intestinal retentive systems – recent advances and emerging approaches

“…Additionally, several in vitro models have been specifically designed to replicate the presence of mucus in the gut, offering accurate and controlled testing environments for mucoadhesive devices. [126][127][128] By incorporating computational analysis, guton-a-chip systems, and specialized in vitro models, researchers can advance the field of IRDs by improving testing methodologies, optimizing device designs, and ultimately facilitating the development of more effective and patient-specific IRDs.…”

Establishment and evaluation of on-chip intestinal barrier biosystems based on microfluidic techniques

“…The mucosal layer serves as a significant barrier during drug delivery. For instance, increasing the intestinal mucosal adhesion of oral drugs can increase drug absorption and permeability and thus boost therapeutic effects [ 192 ]. As shown in Fig.…”