Development of green, high throughput, and sensitive universal microwell spectrofluorimetric method for one-step determination of sartans in their dosage forms and plasma

Darwish, Ibrahim A.; Al-Ghamdi, Abdulmajeed; Al-Hossaini, Abdullah M.

doi:10.1016/j.scp.2023.101325

Cited by 1 publication

(2 citation statements)

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“…Moreover, these manual methods have limited throughputs and fail to adhere the principles of green analytical chemistry (GAC) [24], while also falling short in meeting the demands of high‐throughput analysis required in pharmaceutical and biomedical applications [25]. In contrast, MW‐SFMs performed using fluorescence microplate readers utilize minimal sample and solvent volumes and provide enhanced throughput for the analysis [9, 10]. Therefore, this study aims to develop a MW‐SFM for PGT.…”

Section: Resultsmentioning

confidence: 99%

“…In recent years, spectrofluorimetric measurements employing microwell plates and fluorescence microplate readers have gained prominence. These methods combine the inherent benefits of spectrofluorimetry with the advantages of microwell-based analysis, such as high analytical throughputs, minimum samples/reagents consumption, and environmentally friendly procedures [9][10][11][12]. The favorable attributes of these techniques have motivated us to develop this methodology for PGT.…”

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confidence: 99%

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A green and highly sensitive microwell spectrofluorimetric method with high throughput for the determination of pemigatinib based on dual fluorescence enhancement by photoinduced electron transfer blocking and micellization: Application to the analysis of tablets, content uniformity testing, and human plasma

Darwish,

Alzoman,

Alsalhi

2024

Luminescence

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Pemigatinib (PGT) is a recently FDA‐approved small molecule kinase inhibitor used for the treatment of relapsed or refractory myeloid/lymphoid neoplasms in adults. This study introduces the development of a first microwell spectrofluorimetric method (MW‐SFM) for quantifying PGT in FDA‐approved tablets and plasma samples. The method utilized the enhancement of PGT's weak native fluorescence by blocking photoinduced electron transfer (PET) and micellization with sodium lauryl sulfate (SLS). The MW‐SFM was performed in 96‐microwell plates, and fluorescence signals were measured using a fluorescence microplate reader with excitation at 290 nm and emission at 350 nm. The method exhibited a linear range of 2–250 ng mL−1, and a limit of quantitation was 6.5 ng mL−1. The accuracy and precision of the method were confirmed with recovery rates ranging from 96.5% to 102.8% and relative standard deviations of 1.52% to 3.51%. The MW‐SFM successfully analyzed Pemazyre® tablets, assessed content uniformity, and analyzed PGT‐spiked human plasma samples. The greenness of the MW‐SFM was verified using three different metric tools. In conclusion, the proposed MW‐SFM is a valuable tool in supporting quality assessment of dosage forms, conducting pharmacokinetic studies, and monitoring therapeutic outcomes.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%