Development of granulocytes in haematopoietic tissues of Rhizoprionodon lalandii

Pacheco, Fabio J.; Pacheco, Sos; Segreto, Hrc; Segreto, Roberto Araújo; Silva, Mrr; Egami, Mizue Imoto

doi:10.1111/j.1095-8649.2002.tb01850.x

Cited by 3 publications

(5 citation statements)

References 27 publications

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“…The highly granular appearance reported here for the epigonal organ leukocytes of Prionace in many ways resembles the observations made in the lymphomyeloid tissues of other similarly caught elasmobranchs (Hine & Wain, ,b; Pacheco et al. ), but differs from the rather non‐granular nature of the epigonal organs of the six sexually immature nurse sharks kept for several weeks in holding tanks (Fange & Mattison, ). It should also be noted that the parameters of stress physiology and blood properties measured in long‐line‐captured P. glauca compare favorably with the baseline values of the unstressed sandbar and spiny dogfish sharks (Marshall et al.…”

Section: Discussionsupporting

confidence: 83%

“…Using the immunolocalization of the conserved nuclear factor, PCNA (its immunohistochemistry was previously optimized for shark tissues; Pacheco et al. ; McClusky, , ), it was shown that granule‐lacking leukocytes exclusively infiltrated those cysts that were partially filled with spermatozoa and present in the testis after the wave of wide‐spread MNC apoptotic death in summer‐breeding males (McClusky, ). Blue sharks caught 2 months later possessed, however, fully recovered testes containing numerous intact and spermiating spermatozoal cysts but with no trace of leukocyte infiltration.…”

Section: Discussionmentioning

confidence: 99%

“…) and other elasmobranch species (Pacheco et al. ). The most commonly immunostained nuclei in Prionace epigonal organ were those of blast cells and leukocytes with kidney‐shaped [most likely monocytes (Parish et al.…”

Section: Discussionmentioning

confidence: 99%

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The epigonal organ and mature pole of the testis in the recreationally fished blue shark (Prionace glauca): histochemico‐functional correlates

McClusky

Sulikowski

2014

Journal of Anatomy

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The exact role of the immune system in normal spermatogenesis is poorly understood. The attachment, however, of the lymphomyeloid epigonal organ specifically to the testis's mature pole in many shark species is a curious finding. Unlike the histology of the lymphomyeloid tissues of many other elasmobranchs, the epigonal organ leukocytes of wild-caught blue shark (Prionace glauca), besides exhibiting extensive nuclear heterogeneity, contain some of the largest known granules ever seen in vertebrate white blood cells. It was previously shown that the blue shark epigonal organ remains unremarkable and functionally unchanged despite cestode parasites embedded into its surface, suggesting that it might have other functions in addition to microbial defense. We show here that Prionace epigonal leukocytes shed their granule-laden cytoplasm into the cyst resorption zone (RZ) of the testis, i.e. the region separating the spermatogenic tissue from the epigonal organ, as they begin to migrate into the RZ. Using the immunoreactivity of the conserved transcription factor (proliferating cell nuclear antigen) as marker, it is shown that the granule-lacking leukocytes exclusively infiltrated spermatozoal cysts leftover after the wave of wide-spread multinuclear cell death in summer-breeding males in a seasonally dependent manner. By contrast, Prionace caught 2 months later showed fully recovered testes containing numerous completely intact spermatozoal cysts. Conversely, degenerating immature spermatids were gradually phagocytized by their accompanying Sertoli cells, and leukocytes did not infiltrate such cysts. The autoimmune response described here resembles in every aspect the testicular autoimmune response induced experimentally in a teleost fish. These observations suggest functional adaptation of shark leukocytes in response to specific changes in the testicular microenvironment.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

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The epigonal organ and mature pole of the testis in the recreationally fished blue shark (Prionace glauca): histochemico‐functional correlates

McClusky

Sulikowski

2014

Journal of Anatomy

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“…These selective methods for collagen also demonstrate that the stroma of this organ is made up a fine web of reticular fibres where a large number of cells of the parenchyma are supported, forming a true haematopoietic tissue. This fibrillary web appears to be common in the HK of all teleosts and is also present in other types of organs with haematopoietic function found in Chondrichthyes (Sailendri & Muthukkaruppan, 1975; Sorensen et al , 1997; Pacheco et al , 2002).…”

Section: Discussionmentioning

confidence: 75%

“…For the detection of the expression of the surface antigens DRO45 for T cells, CD68 for macrophages, monocytes and their precursors, and lysozyme for granulocytes, an indirect immunoenzymatic method was used in three steps, utilizing the biotin–streptavidin– peroxidase complex. Sections (3 µm) were placed on silanized slides and dried at 37° C. The slides were then deparaffinized, hydrated, treated in 0·3 M citrate buffer, pH 6·0, placed in a microwave at maximum power for 30 min (UNIFESP/EPM protocol; http://www.unifesp.br) and incubated in an aqueous solution of H 2 O 2 at 50% (Merck) for 15 s to block endogenous peroxidase activity (Pacheco et al , 2002). After washing in distilled H 2 O, the sections were treated in 0·1 M PBS, pH 7·4, for 10 min and incubated with primary antibody diluted 1:80 for CD45RO, 1:100 for CD68, and 1:100 and 1:2000 for lysozyme in bovine serum albumin (BSA) at 1% in PBS overnight, at 3° C in a humidified chamber.…”

Section: Methodsmentioning

confidence: 99%

Morphocytochemical, immunohistochemical and ultrastructural characterization of the head kidney of fat snook Centropomus parallelus

Santos

Gutierre

Antoniazzi

et al. 2011

Journal of Fish Biology

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This study characterized the structure and the morphocytochemical, immunohistochemical and ultrastructural aspects of the head kidney (HK) of the fat snook Centropomus parallelus. The HK is enclosed by a thin capsule of connective tissue, from which fine trabeculae originate and branch into the interior of organ. In the parenchyma, there are aggregates of lymphoid cells containing populations of lymphocytes T immunopositive for CDRO45, in a nodular arrangement, around blood vessels and melano-macrophage centres. Among the cells that constituted these aggregates and surrounded them, were macrophages and monocytes, and their precursors, with strong immunopositivity for CD68, along with cells of the granulocytic lineage in various phases of maturation positive for lysozyme and PAS. Macrophages and chromaffin and interrenal cells are also present. Ultrastructurally, the HK comprises a reticulum-endothelial stroma consisting of endothelial cells, reticulocytes of the fibroblast type and macrophage type and a parenchyma with increased cellularity, principally blood cells of the erythrocytic, granulocytic, lymphocytic, monocytic and thrombocytic series.

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Galeus Melastomus (Chondrichthyes): Reproductive Organs

Porcu¹,

Marongiu²,

Follesa³

2021

Microscopic Anatomy of Animals

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Galeus melastomus females, following the typical elasmobranch pattern, have a reproductive system composed of ovary, anterior and posterior oviducts, oviducal glands, and uteri. However, this species has only a functional right ovary associated with the epigonal organ, a lymphomyeloid tissue, producing different types of blood cells. Information about the microscopic anatomy of epigonal organ, oviducal glands, anterior and posterior oviducts, and uteri are reported. For G. melastomus males, externally characterized by two intromittent organs known as claspers and internally by two equally elongate testes, the histology of the epigonal organ, epididymis, paired deferens ducts, Leydig gland, and seminal vesicle is described.

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Development of granulocytes in haematopoietic tissues of Rhizoprionodon lalandii

Cited by 3 publications

References 27 publications

The epigonal organ and mature pole of the testis in the recreationally fished blue shark (Prionace glauca): histochemico‐functional correlates

The epigonal organ and mature pole of the testis in the recreationally fished blue shark (Prionace glauca): histochemico‐functional correlates

Morphocytochemical, immunohistochemical and ultrastructural characterization of the head kidney of fat snook Centropomus parallelus

Galeus Melastomus (Chondrichthyes): Reproductive Organs

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