Development of gold nanoparticle-based visual assay for rapid detection of Escherichia coli specific DNA in milk of cows affected with mastitis

Deb, Rajib; Pal, Prasanna; Chaudhary, Parul; Bhadsavle, Sanat; Behera, Manisha; Parmanand,; Gautam, Devika; Roshan, Mayank; Vats, Ashutosh; Ludri, Ashutosh; Gupta, Vivek Kumar; De, Sachinandan

doi:10.1016/j.lwt.2021.112901

Cited by 9 publications

(6 citation statements)

References 55 publications

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“…On this platform, magnetoresistive sensors were employed to dynamically detect magnetic beads of 50 nm in diameter connected to Streptococcus agalactiae . Deb et al (2022) [ 194 ] developed an amplification-free visual assay for rapid and sensitive detection of E coli . based on numerous gold nanoparticles (AuNPs) trapped on a magnetic microbead surface.…”

Section: On-farm and Lab Methods For Monitoring Udder Health And Milk...mentioning

confidence: 99%

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Udder Health Monitoring for Prevention of Bovine Mastitis and Improvement of Milk Quality

Neculai-Văleanu¹,

Ariton²

2022

Bioengineering

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To maximize milk production, efficiency, and profits, modern dairy cows are genetically selected and bred to produce more and more milk and are fed copious quantities of high-energy feed to support ever-increasing milk volumes. As demands for increased milk yield and milking efficiency continue to rise to provide for the growing world population, more significant stress is placed on the dairy cow’s productive capacity. In this climate, which is becoming increasingly hotter, millions of people depend on the capacity of cattle to respond to new environments and to cope with temperature shocks as well as additional stress factors such as solar radiation, animal crowding, insect pests, and poor ventilation, which are often associated with an increased risk of mastitis, resulting in lower milk quality and reduced production. This article reviews the impact of heat stress on milk production and quality and emphasizes the importance of udder health monitoring, with a focus on the use of emergent methods for monitoring udder health, such as infrared thermography, biosensors, and lab-on-chip devices, which may promote animal health and welfare, as well as the quality and safety of dairy products, without hindering the technological flow, while providing significant benefits to farmers, manufacturers, and consumers.

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Section: On-farm and Lab Methods For Monitoring Udder Health And Milk...mentioning

confidence: 99%

“…based on numerous gold nanoparticles (AuNPs) trapped on a magnetic microbead surface. The assay was performed without expensive equipment and could detect bacterial DNA as small as 10 2 CFU/μL [ 194 ].…”

Section: On-farm and Lab Methods For Monitoring Udder Health And Milk...mentioning

confidence: 99%

Udder Health Monitoring for Prevention of Bovine Mastitis and Improvement of Milk Quality

Neculai-Văleanu¹,

Ariton²

2022

Bioengineering

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“…The solution was heated for another 10 min until it turned dark red, at which point it was stopped, agitated for another 15 min, cooled, and then stored at 2-8°C. UV/Vis spectroscopy was used to detect the emergence of colloidal gold at a wavelength between 530 and 535 nm [13].…”

Section: Synthesis and Characterization Of Colloidal Gold Nanoparticlesmentioning

confidence: 99%

“…The test membrane was dried for the entire night at 37°C and kept at 25°C. In addition, several doses of 1-2.5 mg/mL of the COVID-19 Nucleocapsid Recombinant Monoclonal Antibody and goat anti-mouse IgG antibody were optimized [13,14].…”

Section: Optimization and Formation Of Antibody-colloidal Gold Conjug...mentioning

confidence: 99%

Development of a colloidal Gold nanoparticle-based lateral-flow assay for rapid detection of SARS-CoV-2 showing enhanced sensitivity and specificity

Suresh¹,

Singha²,

Maheshwari³

et al. 2022

J App Biol Biotech

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To stop the spread of COVID-19 in this outbreak, diagnostic testing is essential. Quick diagnostic tests must be employed in this pandemic, which is brought on by the severe acute respiratory syndrome (SARS)-CoV-2 virus, to successfully treat and manage COVID-19. There are several problems with the present RT-PCR system that the lateral flow assay (LFA), a kind of clinically sensitive diagnostic test, may be able to fix, especially in low-and middleincome nations. Gold nanoparticle-(AuNP-LFA) is a practical method for detecting COVID-19 in basic hospitals and laboratories, particularly in emergency situations where many samples must be quickly examined. Safe, accurate, and non-toxic diagnostic tests must be employed during the pandemic, to successfully treat and manage COVID-19. Recombinant SARS-CoV-2 nucleocapsid monoclonal antibody was employed to detect COVID-19 antigens in the presence of patients to establish a fast LFA for COVID-19. Synthesis of colloidal gold particles and antibody colloidal gold conjugates was evaluated by using UV/Vis spectroscopy. A capture line made of anti-SARS-CoV-2 antibody was coated on nitrocellulose membrane. To create the control line, goat anti-mouse IgG monoclonal antibody was coated. On a polystyrene backing board, the immunochromatographic strip was constructed in the ideal order. Using ELISA as the standard procedure, the strips' sensitivity and specificity were assessed. The results' stability and repeatability were evaluated over a 9-month period. Colloidal gold nanoparticle-based LFAs created in this study can be employed for quicker and more accurate detection of SARS-CoV-2.

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“…Up to date, gold nanoparticles (Au NPs) have been in use for a wide range of biomedical applications like drug delivery, disease treatment, optical imaging and catalysis owing to their physical and chemical properties. [1][2][3][4][5][6][7][8][9][10][11][12] In addition to that, Au NPs have been heavily incorporated to various sensing platform to colorimetrically detect a wide variety of targets including metal/nonmetals ions, [13][14][15][16] organic or biomolecules [17][18][19] and even virus, bacteria and also cancer cells [20][21][22][23][24][25][26] due its unique surface plasmon resonance (SPR) property. For instance, while the dispersed spherical Au NPs in solution gives wine-red color, when they are in close proximity each other, they are in tendency to aggregation and induce blue-purple color.…”

Section: Introductionmentioning

confidence: 99%

Development of a Colorimetric Urease Test Based on Au NPS Capped with Anthocyanin for the Rapid Detection of Helicobacter pylori through Multiple Readouts

Sezgin,

Tekin,

Calim

et al. 2023

ChemistrySelect

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In this work, we have reported use of anthocyanin capped gold nanoparticles (Ant@Au NPs)‐incorporated colorimetric urease test for rapid, sensitive and economic detection of Helicobacter pylori (H. pylori). This test containing Ant@Au NPs, urea, sodium phosphate buffer (PBS) and sodium azide (NaN3) was prepared at pH 5 with red solution owing to dispersity of Ant@Au NPs, urease enzyme secreted from H. pylori cause production of ammonia (NH3) via hydrolysis of urea, then which makes reaction environment alkaline (pH 8.2). The limit of detection for this Ant@Au NPs based urease test was determined to be to 102 CFU/mL with proportional to increasing incubation time. However, distinct color change was observed with 104 CFU/mL H. pylori suspensions in 15 min. The anthocyanin molecules existing on surface of Ant@Au NPs were easily deprotonated from hydroxyl groups in the alkaline condition, which caused various changes on Ant@Au NPs including quite much negative charges on the surface of Ant@Au NPs analyzed by Zeta potential, aggregation of Ant@Au NPs demonstrated by STEM, DLS and spectrophotometer, and turning the color of test solution to purple evaluated by a naked eye and digital process imaging system.

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Development of gold nanoparticle-based visual assay for rapid detection of Escherichia coli specific DNA in milk of cows affected with mastitis

Cited by 9 publications

References 55 publications

Udder Health Monitoring for Prevention of Bovine Mastitis and Improvement of Milk Quality

Udder Health Monitoring for Prevention of Bovine Mastitis and Improvement of Milk Quality

Development of a colloidal Gold nanoparticle-based lateral-flow assay for rapid detection of SARS-CoV-2 showing enhanced sensitivity and specificity

Development of a Colorimetric Urease Test Based on Au NPS Capped with Anthocyanin for the Rapid Detection of Helicobacter pylori through Multiple Readouts

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