Development of GO/Co/Chitosan-Based Nano-Biosensor for Real-Time Detection of D-Glucose

Abstract

Electrochemical nano-biosensor systems are popular in the industrial field, along with evaluations of medical, agricultural, environmental and sports analysis, because they can simultaneously perform qualitative and quantitative analyses with high sensitivity. However, real-time detection using an electrochemical nano-biosensor is greatly affected by the surrounding environment with the performance of the electron transport materials. Therefore, many researchers are trying to find good factors for real-time detection. In this work, it was found that a composite composed of graphite oxide/cobalt/chitosan had strong stability and electron transfer capability and was applied to a bioelectrochemical nano-biosensor with high sensitivity and stability. As a mediator-modified electrode, the GO/Co/chitosan composite was electrically deposited onto an Au film electrode by covalent boding, while glucose oxidase as a receptor was immobilized on the end of the GO/Co/chitosan composite. It was confirmed that the electron transfer ability of the GO/Co/chitosan composite was excellent, as shown with power density analysis. In addition, the real-time detection of D-glucose could be successfully performed by the developed nano-biosensor with a high range of detected concentrations from 1.0 to 15.0 mM. Furthermore, the slope value composed of the current, per the concentration of D-glucose as a detection response, was significantly maintained even after 14 days.

Chitosan–Fe3O4 Nanoparticles Cryogel for Glucose Biosensor Development

“…The low applied potential could avoid the common interference of ascorbic acid and uric acid. This low applied potential was better than previously reported glucose biosensor using chitosan as supporting material such as graphene oxide-cobalt-Chitosan at 0.6V (Kim et al, 2022) , chitosan-Au-Ti nanoparticles at 0.8V (Lipińska et al, 2021) , chitosan-gold nanoparticle at 0.7V (Luo et al, 2004) and chitosan-functionalized graphene at 0.3V (Zou et al, 2019) . Furthermore, both the oxidation and reduction peaks showed linear responses with regression equation of y = 4.5872x + 133.37 for oxidation peaks (Figure 8b) and y = -6.804x -104.32 for reduction peaks (Figure 8c).…”

Optically Active Nanomaterials and Its Biosensing Applications—A Review

“…GO is capable of delivering particular responses to the target molecule and engaging dynamically with the probe [26,27]. Fluorescence, Raman scattering, and electrochemical reaction enable the transduction process.…”

Colorimetric Glucose Biosensor Based on Chitosan Films and Its Application for Glucose Detection in Beverages Using a Smartphone Application

“…Chitosan is a biopolymer widely used for this purpose owing to its biocompatibility, biodegradability, non-toxicity, functional groups for enzyme immobilization, and its ability to create films [ 19 ]. Regarding GOx biosensors and their application in food samples, the biocatalyst has been immobilized on modified electrode surfaces in the presence of chitosan either covalently or through entrapment of the enzyme within a chitosan hydrogel [ 20 , 21 , 22 ]. However, to the best of our knowledge, there is a lack of research on chitosan-based GOx biosensors other than electrochemical ones for glucose determination in food products.…”