Development of glyphosate-tolerant transgenic cotton plants harboring the G2-aroA gene

Zhang, Xiaobing; Tang, Qiaoling; Wang, Xu‐jing; Wang, Zhixing

doi:10.1016/s2095-3119(16)61458-2

Cited by 8 publications

(10 citation statements)

References 23 publications

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“…Three transgenic events were very efficient in regulating shikimate accumulation even at higher concentrations of 4,000 mg/L as depicted by chlorophyll retention. This study is the first of its kind that has shown regulated accumulation of shikimate even at 4,000 mg/L in transgenic events (Rajasekaran et al, 1996a,b;Awan et al, 2015;Liang et al, 2017;Zhang et al, 2017).…”

Section: Discussionmentioning

confidence: 85%

“…Resistance to herbicides was among the first traits for which biotechnological approaches were applied (Wilkins et al, 2000). Biotechnological intervention in cotton improvement programs has been long standing with continued inputs toward many traits, including herbicide tolerance using varied genes (Rajasekaran et al, 1996b;Awan et al, 2015;Liang et al, 2017;Zhang et al, 2017). Nevertheless, this study is the first ever comprehensive report demonstrating development, analysis and efficacy of transgenic cotton with a modified CP4 EPSPS gene.…”

Section: Discussionmentioning

confidence: 90%

“…Crop improvement through genetic engineering has given the feasibility to engineer plants to combat herbicides being sprayed and thus help in the safety of the crops without major yield losses (Benbrook, 2016;Bohn and Lovei, 2017). Various genes that confer herbicide tolerance have been identified, validated, and introgressed into a multitude of crops including cotton (Awan et al, 2015;Liang et al, 2017;Zhang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Transgenic Cotton (Gossypium hirsutum L.) to Combat Weed Vagaries: Utility of an Apical Meristem-Targeted in planta Transformation Strategy to Introgress a Modified CP4-EPSPS Gene for Glyphosate Tolerance

et al. 2020

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Weeds burden plant growth as they compete for space, sunlight, and soil nutrients leading to 25-80% yield losses. Glyphosate [N-(phosphonomethyl)glycine] is a widely used broad spectrum non-selective herbicide that controls weeds by inhibiting 5enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme and interfering with the shikimate biosynthesis pathway. Cotton (Gossypium hirsutum L.) is one of the most important commercial crops grown worldwide for its fiber. We have developed herbicide tolerant transgenic cotton (cv. P8-6) by introgression of a codon-optimized and modified EPSPS gene (CP4-EPSPS) possessing an N-terminal chloroplast targeting peptide from Petunia hybrida. Because of the recalcitrant nature of cotton, a genotype-independent non-tissue culture-based apical meristem-targeted in planta transformation approach was used to develop transformants. Although in planta transformation methodologies are advantageous in developing a large number of transgenic plants, effective screening strategies are essential for initial identification of transformants. In the present study, the use of a two-level rigorous screening strategy identified 2.27% of T1 generation plants as tolerant to 800 and 1,500 mg/L of commercially available glyphosate (Roundup). Precise molecular characterization revealed stable integration, expression, and inheritance of CP4-EPSPS in advanced generations of the promising transgenic events. Further, superiority of selected transgenic plants in tolerating increasing levels of glyphosate (500-4,000 mg/L) was ascertained through reduced accumulation of shikimate. This report is the first of its kind where cotton transformants tolerating high levels of glyphosate (up to 4,000 mg/L) and accumulating low levels of shikimate have been identified. This study not only reiterated the genotype-independent nature of the transformation strategy but also reiterated the translational utility of the CP4-EPSPS gene in management of weeds.

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Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

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Transgenic Cotton (Gossypium hirsutum L.) to Combat Weed Vagaries: Utility of an Apical Meristem-Targeted in planta Transformation Strategy to Introgress a Modified CP4-EPSPS Gene for Glyphosate Tolerance

et al. 2020

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“…In the meantime, several glyphosateinsensitive EPSP synthase mutant variants have been generated by either random or directed mutagenesis or they have been isolated from glyphosate-resistant organisms (Stalker et al, 1985;Eschenburg et al, 2002;Kahrizi et al, 2007;Pollegioni et al, 2011;Chekan et al, 2016). EPSP synthases that are insensitive to glyphosate have been used to genetically engineer glyphosate-tolerant crops (Della-Cioppa et al, 1987;Padgette et al, 1995;Barry et al, 1997;Kahrizi et al, 2007;Cao et al, 2012;Guo et al, 2015;Zhang et al, 2017;Fartyal et al, 2018). strain CP4 and Escherichia coli revealed that glyphosate targets the PEP binding site and acts as a competitive inhibitor of the enzyme (Schönbrunn et al, 2001;Eschenburg et al, 2002;Funke et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…strain CP4 and Escherichia coli revealed that glyphosate targets the PEP binding site and acts as a competitive inhibitor of the enzyme (Schönbrunn et al, 2001;Eschenburg et al, 2002;Funke et al, 2006). EPSP synthases that are insensitive to glyphosate have been used to genetically engineer glyphosate-tolerant crops (Della-Cioppa et al, 1987;Padgette et al, 1995;Barry et al, 1997;Kahrizi et al, 2007;Cao et al, 2012;Guo et al, 2015;Zhang et al, 2017;Fartyal et al, 2018). As stated above, the use of glyphosate-resistant crops in agriculture has substantially contributed to the success and the global market size of the herbicide (Duke and Powles, 2008;Benbrook, 2016).…”

Section: Introductionmentioning

confidence: 99%

Identification of the first glyphosate transporter by genomic adaptation

Wicke

Schulz

Lentes

et al. 2019

Environmental Microbiology

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Summary The soil bacterium Bacillus subtilis can get into contact with growth‐inhibiting substances, which may be of anthropogenic origin. Glyphosate is such a substance serving as a nonselective herbicide. Glyphosate specifically inhibits the 5‐enolpyruvyl‐shikimate‐3‐phosphate (EPSP) synthase, which generates an essential precursor for de novo synthesis of aromatic amino acids in plants, fungi, bacteria and archaea. Inhibition of the EPSP synthase by glyphosate results in depletion of the cellular levels of aromatic amino acids unless the environment provides them. Here, we have assessed the potential of B. subtilis to adapt to glyphosate at the genome level. In contrast to Escherichia coli, which evolves glyphosate resistance by elevating the production and decreasing the glyphosate sensitivity of the EPSP synthase, B. subtilis primarily inactivates the gltT gene encoding the high‐affinity glutamate/aspartate symporter GltT. Further adaptation of the gltT mutants to glyphosate led to the inactivation of the gltP gene encoding the glutamate transporter GltP. Metabolome analyses confirmed that GltT is the major entryway of glyphosate into B. subtilis. GltP, the GltT homologue of E. coli also transports glyphosate into B. subtilis. Finally, we found that GltT is involved in uptake of the herbicide glufosinate, which inhibits the glutamine synthetase.

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Development of Transgenic Cotton for Combating Biotic and Abiotic Stresses

Hussain

Mahmood

2020

Cotton Production and Uses

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Development of glyphosate-tolerant transgenic cotton plants harboring the G2-aroA gene

Cited by 8 publications

References 23 publications

Transgenic Cotton (Gossypium hirsutum L.) to Combat Weed Vagaries: Utility of an Apical Meristem-Targeted in planta Transformation Strategy to Introgress a Modified CP4-EPSPS Gene for Glyphosate Tolerance

Transgenic Cotton (Gossypium hirsutum L.) to Combat Weed Vagaries: Utility of an Apical Meristem-Targeted in planta Transformation Strategy to Introgress a Modified CP4-EPSPS Gene for Glyphosate Tolerance

Identification of the first glyphosate transporter by genomic adaptation

Development of Transgenic Cotton for Combating Biotic and Abiotic Stresses

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