Development of genome‐wide microsatellite resources in a commercially important mussel species (<i>Mytilus chilensis</i>)

Araneda, Cristián; Noriega, Ximena; Jilberto, Felipe; Ruíz, Jorge; Jara, Calvin; Lafarga, Fabiola; Larraín, María Angélica

doi:10.1111/age.12398

Cited by 5 publications

(4 citation statements)

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“…Extracted DNA was quantified using the Quant-iT dsDNA pico-green assay kit (Thermo Fisher Scientific Inc, Waltham, MA) in 96-well plates and a Perkin Elmer Victor 5 plate reader. Since some of the collected individuals were too small (15-25 mm) to identify phenotypic species, these samples were tested with diag-nostic species markers as described by Larra ın et al Araneda et al (2015).…”

Section: Dna Extraction and Species Identificationmentioning

confidence: 99%

Adaptive genetic variation distinguishes Chilean blue mussels (Mytilus chilensis) from different marine environments

Araneda

Larraín

Hecht

et al. 2016

Ecology and Evolution

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Chilean mussel populations have been thought to be panmictic with limited genetic structure. Genotyping‐by‐sequencing approaches have enabled investigation of genomewide variation that may better distinguish populations that have evolved in different environments. We investigated neutral and adaptive genetic variation in Mytilus from six locations in southern Chile with 1240 SNPs obtained with RAD‐seq. Differentiation among locations with 891 neutral SNPs was low (FST = 0.005). Higher differentiation was obtained with a panel of 58 putative outlier SNPs (FST = 0.114) indicating the potential for local adaptation. This panel identified clusters of genetically related individuals and demonstrated that much of the differentiation (~92%) could be attributed to the three major regions and environments: extreme conditions in Patagonia, inner bay influenced by aquaculture (Reloncaví), and outer bay (Chiloé Island). Patagonia samples were most distinct, but additional analysis carried out excluding this collection also revealed adaptive divergence between inner and outer bay samples. The four locations within Reloncaví area were most similar with all panels of markers, likely due to similar environments, high gene flow by aquaculture practices, and low geographical distance. Our results and the SNP markers developed will be a powerful tool supporting management and programs of this harvested species.

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Section: Dna Extraction and Species Identificationmentioning

confidence: 99%

Adaptive genetic variation distinguishes Chilean blue mussels (Mytilus chilensis) from different marine environments

Araneda

Larraín

Hecht

et al. 2016

Ecology and Evolution

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“…In many parts of the world mussels are an important source of protein, both in terms of wild harvest and aquaculture production [22–24]. Over the last 50 years or so, a better global understanding of the phylogeography, taxonomy and systematics of smooth-shelled blue mussels of the genus Mytilus has been achieved with a range of genetic marker types, from allozymes (protein variation) [25–30], through mitochondrial DNA (mtDNA) sequencing and restriction fragment length polymorphisms (RFLPs) [31–42], to nuclear DNA markers [43–48], such as microsatellites [49–55] and, most recently, single nucleotide polymorphisms (SNPs) [56–61]. The development and application of each new generation of molecular marker type has provided new insight into the phylogeography and the biosecurity threat of blue mussels in a global perspective.…”

Section: Introductionmentioning

confidence: 99%

Cryptic diversity in smooth-shelled mussels on Southern Ocean islands: connectivity, hybridisation and a marine invasion

2019

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Background Large numbers of endemic species inhabit subantarctic continental coasts and islands that are characterised by highly variable environmental conditions. Southern hemisphere populations of taxa that are morphologically similar to northern counterparts have traditionally been considered to be extensions of such Northern hemisphere taxa, and may not exhibit differentiation amongst geographically isolated populations in the Southern Ocean. Smooth-shelled blue mussels of the genus Mytilus that exhibit an anti-tropical distribution are a model group to study phylogeography, speciation and hybridisation in the sea, and contribute to the theory and practice of marine biosecurity. Methods We used a single nucleotide polymorphism (SNPs) panel that has the ability to accurately identify reference Northern and Southern hemisphere Mytilus taxa to test for evolutionary differentiation amongst native Southern Ocean island populations. Results Native mussels from the Falkland Islands and the Kerguelen Islands exhibited greatest affinity to native M. platensis d’Orbigny 1846 from the Atlantic coast of South America. The major Southern Ocean current flow from west to east is likely to explain the spreading of M. platensis to remote offshore islands, as adults via the process of rafting or perhaps directly as larvae. SNPs variation revealed that mussels from Tasmania were native and clearly differentiated from all other blue mussel groups in the Southern and Northern hemispheres. The native mussels M. planulatus from Tasmania and from mainland New Zealand (NZ), and tentatively M. aoteanus from the two NZ Southern Ocean offshore island groups (the Auckland Islands and Campbell Island), formed a distinct M. galloprovincialis –like Southern hemisphere group with closest affinity to Northern hemisphere M. galloprovincialis from the Mediterranean Sea. In all cases, the SNPs revealed evidence of hybridisation between two or more distinct taxa. The invasive Northern hemisphere M. galloprovincialis was identified only in Tasmania, amongst native mussels of a distinct Australian M. planulatus lineage. Conclusion Overall, our results reveal that Southern hemisphere island mussels have mixed genome ancestry and are native, not introduced by human activities. The preservation of distinct evolutionary lineages of Southern hemisphere species needs to be an ongoing focus of conservation efforts, given that population sizes on some of the remote offshore oceanic islands will be small and may be more easily adversely affected by invasion and subsequent hybridisation and introgression than larger populations elsewhere. Electronic supplementary material ...

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“…A limitation of this study is the small number of microsatellite loci available for use. Surprisingly for such an ecologically and economically important species group, there has been only a limited number of microsatellite loci developed for use with the Mytilus species complex (Araneda et al, 2016; Diz & Presa, 2008, 2009; Giantsis et al, 2014; Larraín et al, 2015), and a limited number of studies investigating population genetic structure of M. galloprovincialis using microsatellites. Interestingly, markers have often not performed well when used on mussels from different geographic regions, with, for example, only a subset of markers developed for western Mediterranean mussels (Diz & Presa, 2008), performing satisfactorily for eastern Mediterranean mussels (Giantsis et al, 2014; Štambuk et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Limited population genetic variation but pronounced seascape genetic structuring in populations of the Mediterranean mussel (Mytilus galloprovincialis) from the eastern Adriatic Sea

Hamilton

Piria

Gavrilović

et al. 2023

Ecology and Evolution

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Population genetic analysis of variation at five neutral microsatellite loci for Mediterranean mussels (Mytilus galloprovincialis) from 18 sites along the eastern Adriatic Sea revealed little or no spatial variation. In contrast, seascape genetics analysis revealed a pronounced locus-specific gradient in allelic and genotypic frequencies across the study region. At a sixth locus, MGE7, the frequencies of two alleles, MGE7 243 and MGE7 249 , were strongly associated, negatively and positively, respectively, with a single environmental variable -minimum salinity (minSAL). The frequency of the MGE7 243/243 homozygous genotype was strongly negatively associated with minSAL, whereas the frequencies of the MGE7 246/249 and the MGE7 249/249 genotypes were strongly positively correlated with minSAL. Interpretation of these pronounced gradients is confounded by the fact that minSAL and another environmental variable, maximum sea surface temperature (maxSST), are highly correlated (R = −.911) and are therefore not necessarily acting independently. BLAST searches of the MGE7 locus against M. galloprovincialis whole genome shotgun sequence returned an alignment with contig mg10_S01094 (accession UYJE01010330.1) and 7 predicted M. galloprovincialis proteins VDI82194.1 -VDI82200.1. Conserved domain searches revealed a similar structure to the transcriptional regulator Msx2-interacting protein.The BLASTp search also returned significant alignments to Msx2-interacting proteins in Mytilus coruscus, Crassostrea virginica, and Haliotis rubra. The existence of the MGE7 gradient highlights the role that environmental variation may play in retarding gene flow among wild M. galloprovincialis populations, and also how the success of collection of young mussels (spat) from one site and their transfer to another site (the farm) may be influenced by a single factor such as minSAL or maxSST on a localized scale.

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Development of genome‐wide microsatellite resources in a commercially important mussel species (Mytilus chilensis)

Abstract: Conicyt-Fondecyt 113030

Cited by 5 publications

References 5 publications

Adaptive genetic variation distinguishes Chilean blue mussels (Mytilus chilensis) from different marine environments

Adaptive genetic variation distinguishes Chilean blue mussels (Mytilus chilensis) from different marine environments

Cryptic diversity in smooth-shelled mussels on Southern Ocean islands: connectivity, hybridisation and a marine invasion

Limited population genetic variation but pronounced seascape genetic structuring in populations of the Mediterranean mussel (Mytilus galloprovincialis) from the eastern Adriatic Sea

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