Development of Genetically Encoded Fluorescent KSR1-Based Probes to Track Ceramides during Phagocytosis
Vladimir Girik,
Larissa van Ek,
Isabelle Dentand Quadri
et al.
Abstract:Ceramides regulate phagocytosis; however, their exact function remains poorly understood. Here, we sought (1) to develop genetically encoded fluorescent tools for imaging ceramides, and (2) to use them to examine ceramide dynamics during phagocytosis. Fourteen enhanced green fluorescent protein (EGFP) fusion constructs based on four known ceramide-binding domains were generated and screened. While most constructs localized to the nucleus or cytosol, three based on the CA3 ceramide-binding domain of kinase supp… Show more
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