1979
DOI: 10.1016/0009-8981(79)90379-6
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Development of gel filtration and specific analyses of urinary carbohydrate and protein material

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Cited by 14 publications
(4 citation statements)
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“…11) which shows a similar pattern of accumulation and decline is hydrolyzed to G6 and G2. This evidence is supported by work on the susceptibility of individual oligosaccharides to a-amylase attack [3]. An essential difference between the hydrolysates of the same D.E.…”
Section: Resultsmentioning
confidence: 79%
See 1 more Smart Citation
“…11) which shows a similar pattern of accumulation and decline is hydrolyzed to G6 and G2. This evidence is supported by work on the susceptibility of individual oligosaccharides to a-amylase attack [3]. An essential difference between the hydrolysates of the same D.E.…”
Section: Resultsmentioning
confidence: 79%
“…11) than in combination with pullulanase (b). It is likely that this distinguishing characteristic derives from a restricted access to the branched polysaccharide which a-amylase experiences in regime (a) and which results in a larger rate of hydrolysis of the oligosaccharides such as G7 and G8, which are amenable to aamylase [3], than when pullulanase is present (b) to provide a more accessible polysaccharide substrate, by the specific hydrolysis of the a-(1+ 6) linkages. If this is the case then these differences between the product spectra of regimes (a) and (b) should be considerably enlarged by the use of a more highly branched substrate.…”
Section: Resultsmentioning
confidence: 99%
“…A water jacketted column (140 x 2.8 cm id) maintained at 60°C, to eliminate hydration effects relating to the gel, [lo, 211, using a thermostatted circulatory bath (Grant FH15) was packed with Bio-Gel P2 (-400 mesh). The packed column was continuously pumped using a piston pump (Metering Pumps Ltd, Series 11) at constant flow rate (0.86ml min-') with sodium chloride solution (0.1 M) to prevent the occurrence of any nonspecific adsorption phenomenon [13,22]. Column eluents were continuously monitored using an automated L-cysteine/sulphuric acid assay [23,241.…”
Section: Analysis Using Gel Permeation Chromatographymentioning
confidence: 99%
“…Polyacrylamide gel media, first introduced by Hjerten and Mosbach [7] has the distinct advantages over cross-linked dextran gels [8] for such separations in that adsorption of sample to dextran is common [9], and polyacrylamide is less biodegradable. The most common technique for the separation of oligosaccharides by gel permeation chromatography was first introduced by John et al [lo], and has found widespread applications including the analysis of corn syrups [ll], malto-oligosaccharides in beer [12], and urine for carbohydrate and protein as a diagnostic method for the determination of some genetic diseases [13] to name but a few applications. There have however been sporadic citings in the literature that shoulders on some peaks are observed in starch hydrolysates [14,15] and this has been explained in terms of branched oligosaccharides in which there are both (1 + 4)-a-D and (1 + 6)-a-D glycosidic linkages present.…”
Section: Introductionmentioning
confidence: 99%