Development of GCxGC/TOF-MS metabolomics for use in ecotoxicological studies with invertebrates

Ralston-Hooper, Kimberly; Hopf, Amber; Oh, Cheolhwan; Zhang, Xiang; Adamec, Jiřı́; Sepúlveda, Marı́a S.

doi:10.1016/j.aquatox.2008.03.002

Cited by 79 publications

(53 citation statements)

References 23 publications

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“…Each 20l sample was derivatized by adding 20l of N-tertbutyldimethylsilyl-N-methyltrifluoroacetamide (TBDMS, SigmaAldrich, St Louis, MO, USA), followed by heating for 1h at 60°C, to generate a dimethyl tertbutylsilane (DMTBS) analog. Analysis was performed using a Pegasus 4D GCϫGC/TOF-MS (LECO Corporation, St Joseph, MI, USA), utilizing conditions similar to those reported elsewhere (Ralston-Hooper et al, 2008). Briefly, the first dimension (1D) column was a non-polar DB-5 capillary column (J&W Scientific, 30mϫ0.25mmϫ0.25m), coupled to a second dimension (2D) medium DB-17 capillary column (J&W Scientific, 1mϫ0.10mmϫ0.10m) by a glass union.…”

Section: Metabolite Extraction and Derivatizationmentioning

confidence: 99%

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Evaluation of a tandem gas chromatography/time-of-flight mass spectrometry metabolomics platform as a single method to investigate the effect of starvation on whole-animal metabolism in rainbow trout (Oncorhynchus mykiss)

Baumgarner

Cooper

2012

Journal of Experimental Biology

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SUMMARY This study was conducted to evaluate the use of a two-dimensional gas chromatography/time-of-flight mass spectrometry (GC×GC/TOF-MS) metabolomic platform to comprehensively analyze the effect of starvation on whole-animal metabolism in rainbow trout (Oncorhynchus mykiss). Trout were either fed a commercial diet at 2% body mass twice daily or starved for 4 weeks. Metabolomic analysis was conducted on serum, liver and muscle tissue from each fish. Database searching and statistical analysis revealed that concentrations of more than 50 positively identified molecules changed significantly (P<0.05) as a result of starvation. Our results indicate that starving rainbow trout for 4 weeks promotes increased utilization of select tissue fatty acids in liver and muscle. However, starvation did not significantly affect protein catabolism in peripheral tissues, as indicated by reductions in the level of serum amino acids in starved fish. In contrast, starvation appears to promote protein catabolism in liver as the level of methionine, proline and lysine metabolite 2-piperidine carboxylic acid increased significantly. Also, starvation resulted in significant changes in the level of numerous xenobiotics that could indicate the origin of particular feed ingredients and selective retention of these molecules in tissues. We suggest that metabolomic analysis using GC×GC/TOF-MS is an effective tool in studying whole-animal metabolism and the fate of important xenobiotic compounds in rainbow trout as numerous polar and non-polar metabolites were rapidly and accurately profiled using a single method.

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Section: Metabolite Extraction and Derivatizationmentioning

confidence: 99%

“…Normalization was executed using R (v. 2.6.0, www.r-project.org) statistical software. Normalized peaks were then subjected to statistical analysis using software (MSort ® ) to align, match and compare metabolite peaks from various samples and groups (Ralston-Hooper et al, 2008).…”

Section: Metabolite Identification and Peak Normalizationmentioning

confidence: 99%

Evaluation of a tandem gas chromatography/time-of-flight mass spectrometry metabolomics platform as a single method to investigate the effect of starvation on whole-animal metabolism in rainbow trout (Oncorhynchus mykiss)

Baumgarner

Cooper

2012

Journal of Experimental Biology

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“…The sample was transferred to an auto sampler vial and immediately analyzed. The sample was analyzed under the instrumental conditions outlined by [13].…”

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confidence: 99%

Phytochemical Screening, GCxGC TOF-MS Analysis and Antibacterial Properties of Crude Rhoicissus Ttomentosa Rhizome Extract

2016

International Conference on Advances in Science, Engineering, Technology and Natural Resources (ICASETNR-16) Nov. 24-25, 2016 P

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Abstract-Rhoicissus tomentosa is a plant used in South Africantraditional medicine to treat ailments mainly related to fertility and reproduction. Phytochemical screening was carried out and the antimicrobial activity of the rhizomes of R. tomentosa was investigated for the first time to ascertain their possible pharmaceutical potential. The results showed that the rhizomes of the plant contain alkaloids, flavonoids, saponins, steroids, reducing sugars and tannins. GCxGC-TOF-MS analysis of the extracts displayed the presence of over 100 known bioactive compounds, many reported here and detected in Rhoicissus tomentosa for the first time. Methanol: chloroform (50/50, v/v) and ethyl acetate (100%) extracts of the rhizomes were tested against 14 bacterial strains using the disc diffusion and microdilution assay methods. Bacteria most susceptible to rhizome extracts were Staphylococcus aureus (0.063 mg/mL) and Bacillus subtilis (0.125 mg/mL). The results obtained show that the rhizomes of R. tomentosa have antimicrobial activity against a number of pathogenic microorganisms.

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“…It is an emerging technology that provides considerably more chemical selectivity than GC-MS. It uses two capillary GC columns connected via a thermal modulator to achieve a high degree of separation of metabolites [136][137][138]. Compared to the 15-60 m length of the first dimension column, the length of the second dimension column is very short, in general only 0.5-2 m. These two GC columns usually have different stationary phases to maximize the orthogonality of the two-dimensional separation system to achieve high peak capacity.…”

Section: Introductionmentioning

confidence: 99%

“…Comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC×GC-TOF MS) is a powerful analytical platform in metabolomics. It uses two distinctly different capillary GC columns of different polarities connected via a thermal modulator [137,138,175]. The analytes co-eluted from the first column are further separated in the second column because of the difference of column temperature and the chromatographic polarities.…”

Section: Introductionmentioning

confidence: 99%

Biomarker discovery of liver diseases using mass spectrometry-based metabolomics.

Shi¹

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Metabolomics has emerged as one of the latest of the "-omics" disciplines that can detect metabolite biomarkers in biological samples. The diverse characteristics of metabolites make the analytical platform challenging in metabolomics. Two bioanalytical platforms were developed in this study to investigate metabolite abundance changes under different biological conditions. We first developed a bioanalytical platform that coupled linear trap quadruple -Fourier transform ion cyclotron mass spectrometer (LTQ-FTICR MS) with direct infusion chip-based nano-electrospray ionization (DI-nESI) and applied it to study polychlorinated biphenyls (PCB) effects on non-alcoholic fatty liver disease (NAFLD).We also employed this platform in conjunction with in vivo metabolite deuterium labeling to study whether chronic alcohol exposure disturbs lipid homeostasis by analyzing triacylglycerol regulation alteration in adipose tissue and liver tissue. We further developed a comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC×GC-TOF MS) platform for metabolomic profiling and applied it to study the effects of arsenic exposure in a mouse model of diet-induced fatty liver disease, as well as the effects of Lactobacillus rhamnosus GG on alcoholic fatty liver vii disease (AFLD) in mice. The results of our metabolomics analyses agreed with the results of histological studies and provided molecular level information for further understanding the mechanisms of liver diseases.viii

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Development of GCxGC/TOF-MS metabolomics for use in ecotoxicological studies with invertebrates

Cited by 79 publications

References 23 publications

Evaluation of a tandem gas chromatography/time-of-flight mass spectrometry metabolomics platform as a single method to investigate the effect of starvation on whole-animal metabolism in rainbow trout (Oncorhynchus mykiss)

Evaluation of a tandem gas chromatography/time-of-flight mass spectrometry metabolomics platform as a single method to investigate the effect of starvation on whole-animal metabolism in rainbow trout (Oncorhynchus mykiss)

Phytochemical Screening, GCxGC TOF-MS Analysis and Antibacterial Properties of Crude Rhoicissus Ttomentosa Rhizome Extract

Biomarker discovery of liver diseases using mass spectrometry-based metabolomics.

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