Development of Four Uv-Spectrometric Techniques for Concurrent Estimation of Aspirin and Sildenafil Citrate in Their Binary Mixture and Pharmaceutical Formulations

Abstract

The combination of aspirin (ASP) and sildenafil citrate (SIL) into single dosage form has been of interest for a considerable period; because it has a synergistic effect for the handling of many diseases. Therefore, UV-spectrometric techniques were desperately required for the concurrent estimation of ASP and SIL in their binary mixture and pharmaceutical formulations. In this paper, four simple, low-cost and precise UV-spectrometric techniques were evolved for the concurrent estimation of ASP and SIL. The advanced methods include treatment of the zeroorder spectrum by ratio subtraction (RS) along with spectrum subtraction method (SS), absorption correction method (ACM), area under the curve correction method (AUCCM) and induced dual wavelength method (IDWM). Moreover, UV-spectrometric purity analysis via spectral ratio factor (SRF) was developed; which considered the first UV-spectrometric purity testing to verify the purity of ASP in pharmaceuticals formulations. All approaches were linear; in the range of 25.0-100.0 μg/mL for ASP, and 10.0-50.0 μg/mL for SIL. The mean percentage recoveries for ASP were 100.92, 100.33, 100.79 and 100.18% respectively, and for SIL were 99.27, 99.56, 99.83 and 99.44% respectively. The SRF values were approximating unity and showed high purity of the resolved peaks. The results were statistically compared with the HPLC reported method and no significant variance was discerned.

Spider diagram and sustainability evaluation of UV-methods strategy for quantification of aspirin and sildenafil citrate in the presence of salicylic acid in their bulk and formulation

“… Drug analyzed Method studied Publication year Reference Aspirin + Methocarbamol in the apparition of guaifenesin and salicylic acid. HPLC-DAD method 2019 [ 9 ] Aspirin + Omeprazole in the apparition of Salicylic Acid Spectrophotometric methods 2022 [ 10 ] Aspirin + Omeprazole in the presence of salicylic acid UHPLC and TLC 2021 [ 11 ] Aspirin + Salicylic acid + Salvianolic acid B UPLC-MS/MS 2021 [ 12 ] Sildenafil citrate + Dapoxetine + Vardenafil + Tadalafil Spectrofluorimetric and Spectrophotometric methods 2020 [ 15 ] Dapoxetine + Sildenafil Spectrophotometric methods 2022 [ 16 ] Sildenafil citrate Infrared Spectroscopic method 2021 [ 17 ] Sildenafil HPLC 2020 [ 18 ] Aspirin + Sildenafil citrate Spectrophotometric method 2015 [ 19 ] Aspirin + Sildenafil citrate Spectrophotometric methods 2022 [ 20 ] Aspirin + Sildenafil citrate + Salicylic acid HPLC 2014 [ ...…”

“…With respect to the quantification of ASA and SIC, a review of the literature found two spectrophotometric approaches for their synchronous quantification [ 19 , 20 ], as shown in Table 1 . However, none of these preceding spectrophotometric studies analyzed these drugs in the presence of salicylic acid (SA) Fig.…”

Method Development for Simultaneously Determining Indomethacin and Nicotinamide in New Combination in Oral Dosage Formulations and Co-Amorphous Systems Using Three UV Spectrophotometric Techniques

“…In total, three UV spectrophotometric methods were developed. Tese methods have been proven to be accurate, precise, fast, simple, and eco-friendly for many combinations and multicomponent dosage formulations without using any hazardous solvents or invasive materials [30][31][32][33][34][35]. Additionally, more advanced UV spectrophotometric methods have been developed initially from these reported ones deploying further mathematical processing [36][37][38].…”