Development of formulations of<i>Trichoderma harzianum</i>strain M1 for control of damping-off of tomato caused by<i>Pythium aphanidermatum</i>

Jayaraj, J.; Radhakrishnan, N.; Velazhahan, R.

doi:10.1080/03235400500094720

Cited by 67 publications

(37 citation statements)

References 20 publications

(18 reference statements)

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“…In India, many biological control agents are regulated by the Provincial Government and are available as subsidized inputs, which are delivered to farmers by Government owned outlets early in the cropping season. Furthermore, farmers are always advised to use fresh formulations (Jeyarajan et al, 1993;Jayaraj et al, 2005). Consequently, this system should overcome any problems related to shelf-life of the formulations.…”

Section: Formulations Ingredients/methodsmentioning

confidence: 98%

Characterization of a Pseudomonas fluorescens strain from tomato rhizosphere and its use for integrated management of tomato damping-off

2007

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A highly antagonistic Pseudomonas fluorescens strain was isolated from tomato rhizosphere and characterized for its in vitro and in vivo biocontrol potential against Pythium aphanidermatum. The identified Pseudomonas fluorescens strain (PfT-8) was capable of producing high levels of chitinase, b-1,3-glucanase, cellulase, fungitoxic metabolites and siderophores. Seven different carrier formulations including a talcbased powder, lignite-based powder, peat-based powder, lignite + fly ash-based powder, wettable powder, bentonite-paste and polyethylene glycol (PEG) paste were prepared utilizing PfT-8. Shelf life was evaluated for up to 6 months of storage at ambient room temperature (28°C). Biocontrol efficacy of formulations was studied under greenhouse and field conditions. The formulations were stable up to at least 2 months of storage at ambient room temperature. Among the formulations, peat, lignite, lignite+fly-ash and bentonite paste based formulations maintained higher propagule number than others and also showed greater biocontrol potential. However, propagule number gradually decreased with time. Several organic amendments including farm yard manure (FYM), leaf-compost, poultry manure, press mud, vermi-compost and neem cake were incorporated into soil to study their influence on P. fluorescens colonization in the rhizosphere and on potential disease control. Soil incorporation of organic amendments and specifically poultry manure and FYM, significantly reduced damping-off incidence and also augmented the rhizosphere population of the marked P. fluorescens strain that was resistant to streptomycin and rifampicin. An integrated approach of damping-off management employing dual inoculation of PfT-8 in seed and soil coupled with soil application of organic amendments including poultry manure or FYM was evaluated under field conditions. Under these conditions, damping-off incidence substantially reduced by up to 90% and further the healthy plant BioControl (2007) 52:683-702 Ó IOBC 2007 stand, plant biomass and plant rhizosphere population of P. fluorescens increased significantly.

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Section: Formulations Ingredients/methodsmentioning

confidence: 98%

Characterization of a Pseudomonas fluorescens strain from tomato rhizosphere and its use for integrated management of tomato damping-off

2007

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“…Growth inhibition of Pythium species by the Trichoderma metabolites has been well researched (15,18). The successful application of Trichoderma species for the management of damping-off caused by Pythium species in chilli and tomato has been previously reported (14,19). The objectives of the present study were (1) Isolation and identification of pathogen, (2) Isolation of Trichoderma species from chilli rhizosphere and tested for its efficacy against P. aphanidermatum (3) To study the morphological characteristics of Trichoderma species (4) Efficacy of seed treatment with Trichoderma species on chilli seedling growth.…”

Section: Introductionmentioning

confidence: 99%

Exploitation of trichoderma species on the growth of Pythium Aphanidermatum in Chilli

Muthukumar

Eswaran

Sanjeevkumas

2011

Braz. J. Microbiol.

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Damping-off of chilli caused by Pythium aphanidermatum is a major nursery disease in vegetables. In vitro experiments evaluated the effect of eight isolates of Trichoderma species (from chilli rhizosphere) were tested against P. aphanidermatum. All the Trichoderma species had varied antagonistic effects against the pathogen. Among them, TVC 3 recorded maximum growth inhibition of P. aphanidermatum and produced more amounts of volatile and non-volatile metabolites. The culture filtrate of the Trichoderma isolate TVC 3 recorded complete inhibition on the mycelial growth of pathogen at 15% concentration. Moreover, chilli seeds treated with culture filtrate of the isolate TVC 3 recorded maximum germination percentage, shoot length, root length and vigour index of chilli. The study identified the Trichoderma isolate (TVC 3 ) performed well in inhibiting the mycelial growth of pathogen as well as increased the plant growth in chilli.

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“…Up to 74 % reduction in disease incidence was recorded when Trichoderma formulation was applied as seed treatment. Additionally, enhanced plant biomass under field and greenhouse conditions was also recorded (Jayaraj et al 2006). Pelletized formulations of kaolin clay and wheat bran in an alginate gel containing conidia, fermentor biomass, or chlamydospores of G. virens and Trichoderma spp.…”

Section: Granular Formulationsmentioning

confidence: 99%

Formulation Technology of Biocontrol Agents: Present Status and Future Prospects

Keswani

Bisen

Singh

et al. 2016

Bioformulations: For Sustainable Agriculture

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Development of formulations ofTrichoderma harzianumstrain M1 for control of damping-off of tomato caused byPythium aphanidermatum

Cited by 67 publications

References 20 publications

Characterization of a Pseudomonas fluorescens strain from tomato rhizosphere and its use for integrated management of tomato damping-off

Characterization of a Pseudomonas fluorescens strain from tomato rhizosphere and its use for integrated management of tomato damping-off

Exploitation of trichoderma species on the growth of Pythium Aphanidermatum in Chilli

Formulation Technology of Biocontrol Agents: Present Status and Future Prospects

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