Abstract:We presented a flow cytometry protocol to isolate and measure the cellular component of tumour immune microenvironment (TIME) of mice bearing breast cancer models. The immune cells infiltrating the tumour microenvironment (TME) have been getting more attention as the determination of their pro- or/and anti-tumorigenic activity contributes to cancer treatment success. In addition, our group has a strong interest to investigate the infiltration of eosinophils within the TME as their tumorigenic role is still unc… Show more
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