This study was conducted to evaluate the alcohol-producing abilities and
functional characteristics of five strains of non-Saccharomyces
cerevisiae (NSC), namely, Kluyveromyces marxianus
KJ-L, Wickerhamomyces anomalus KJ-1, W.
anomalus CP-2, Pichia anomalus HAN-1, and
Meyerozyma guilliermondii SU-L, which were isolated from
Korean traditional nuruk. The selected yeast strains produced
large amounts of alcohol using a carbon source of 20% glucose and were
identified as NSC based on their 18S rDNA sequences. The optimal growth
temperature and pH of the NSC except for M. guilliermondii SU-L
were 25°C and 4.0, respectively. Two NSC strains, KJ-L and CP-2, produced
5–10% more alcohol compared to that produced by S. cerevisiae
LP (SC LP) control. Although the α-amylase and
ß-glucosidase activities were slightly lower in the NSC strains than in
the SC LP, glucoamylase activity was 1.4-fold higher in the NSC strains than in
the SC LP. The α-glucosidase inhibition activity
evaluated using 1% acarbose was 29-fold higher for K. marxianus
KJ-L and P. anomalus HAN-1 than that of SC LP. The five strains
of NSC exhibited higher angiotensin-converting enzyme inhibitory activity with
inhibition rates of 51.7–61.7% compared to that of SC LP (37.3%). The tyrosinase
inhibition activities of NSC (86.4-91.5%) and SC LP (94.9%) were significantly
higher than that of the positive control, kojic acid. These NSC yeast show high
potential for industrial application as domestic starters for manufacturing
fermented foods and cosmetics based on their physiological activities and
functional characteristics.