Development of fast photoacoustic microscopy system for small animal brain imaging

Zafar, Mohsin; Matchynski, James I.; Manwar, Rayyan; Ranjbaran, Seyed Mohsen; Conti, Alana C.; Perrine, Shane A.; Avanaki, Kamran

doi:10.1117/12.2581244

Cited by 7 publications

(7 citation statements)

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“…The signals were generated in LabVIEW and fed to the galvanometer using an output card (BNC 2110, National Instruments, TX). The details of the spiral scanning mechanism can be found in our prior works [3,20,21]. A frame trigger signal (5 V peak-peak square wave, 0.15625 Hz frequency, and 50% duty cycle) was also generated from LabVIEW to trigger data acquisition upon rising edge.…”

Section: Sls-pam Experimental Setupmentioning

confidence: 99%

Ultra‐widefield and high‐speed spiral laser scanning OR‐PAM: System development and characterization

Zafar

Manwar

McGuire

et al. 2023

Journal of Biophotonics

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Photoacoustic microscopy (PAM) is a high-resolution imaging modality that has been mainly implemented with small field of view applications. Here, we developed a fast PAM system that utilizes a unique spiral laser scanning mechanism and a wide acoustic detection unit. The developed system can image an area of 12.5 cm 2 in 6.4 s. The system has been characterized using highly detailed phantoms. Finally, the imaging capabilities of the system were further demonstrated by imaging a sheep brain ex vivo and a rat brain in vivo.

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Section: Sls-pam Experimental Setupmentioning

confidence: 99%

Ultra‐widefield and high‐speed spiral laser scanning OR‐PAM: System development and characterization

Zafar

Manwar

McGuire

et al. 2023

Journal of Biophotonics

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“…The NI system is controlled with LabVIEW and equipped with two 14-bit, eight-channel digitizer cards (NI PXIe-5170R, National Instruments). Experimental setup is further explained in our other works [8]. The sampling rate of the DAQ is set to 41.7 MS/s.…”

Section: Pam System Setupmentioning

confidence: 99%

High‐fidelity compression for high‐throughput photoacoustic microscopy systems

Zafar

Manwar

Avanaki

2022

Journal of Biophotonics

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Photoacoustic microscopic images can assist specialists in disease diagnosis by providing vascular information. However, the size of such data is usually extremely large (ie, gigabytes), and thus, a real-time, efficient compression method can facilitate easy storage and transportation of these images. We have implemented multiple data compression methods in LabVIEW with a high compression ratio and execution times below the repetition rate of the pulsed laser. The qualitative and quantitative results of ex vivo and in vivo imaging with compression showed near-identical images to uncompressed images, with significantly smaller size.

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“…This reduces our capability to correct aberrations and efficiently use non-invasive imaging method like TCUS. Some other imaging modalities such photoacoustic imaging (both in tomography and microscopy) [12,[42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57] also suffers from such limitations.…”

Section: Introductionmentioning

confidence: 99%

Relationship between skull phantoms physical characteristics and acoustic wave signal features

Saint-Martin¹,

Aggrawal²,

Manwar³

et al. 2023

Photons Plus Ultrasound: Imaging and Sensing 2023

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During medical investigations of the head, ultrasound measurements can offer information with simple, non-invasive, and real-time procedure. However, for human adult applications, the clinical treatment of transcranial acoustic imaging remains a challenge by the presence of the skull, results in acoustic aberrations caused by two main phenomena, i.e., attenuation and distortion. These aberrations may affect the signal understanding because of the induced artifacts and the inaccuracy of the imaging target structural information. Variations of the physical properties of the skull, its thickness and porosity, will strongly affect the mechanical properties of the medium and thus the acoustic response. We propose a method to understand the influence of these characteristics on the signal degradation. In order to mimic the human adult skull, a large quantity of epoxy resin-based phantoms is created to explore all the possible physical characteristic variation in the bone. Additional components, titanium dioxide and seeds, will be added to the samples to recreate the acoustic scattering effects of a skull bone. Signal features from pulse-echo mode ultrasound, such as signal attenuation or broadening, will be extracted and studied in the time and frequency domain. In this paper, we are looking for relationship between these physical parameters and the signal features, with the objective to determine bone characteristics without any direct access in later experiments; and going a step further into aberration correction during transcranial imaging procedure.

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Development of fast photoacoustic microscopy system for small animal brain imaging

Cited by 7 publications

References 0 publications

Ultra‐widefield and high‐speed spiral laser scanning OR‐PAM: System development and characterization

Ultra‐widefield and high‐speed spiral laser scanning OR‐PAM: System development and characterization

High‐fidelity compression for high‐throughput photoacoustic microscopy systems

Relationship between skull phantoms physical characteristics and acoustic wave signal features

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